Epigallocatechin gallate (EGCG) attenuates staphylococcal alpha-hemolysin (Hla)-induced NLRP3 inflammasome activation via ROS-MAPK pathways and EGCG-Hla interactions.
Animals
Anti-Inflammatory Agents
/ pharmacology
Antioxidants
/ pharmacology
Bacterial Proteins
/ genetics
Catechin
/ analogs & derivatives
Disease Models, Animal
Hemolysin Proteins
/ genetics
Hemolysis
/ drug effects
Humans
Inflammasomes
/ metabolism
Male
Mice, Inbred C57BL
Mitogen-Activated Protein Kinases
/ metabolism
NLR Family, Pyrin Domain-Containing 3 Protein
/ metabolism
Oxidative Stress
/ drug effects
Reactive Oxygen Species
/ metabolism
Signal Transduction
Staphylococcal Infections
/ drug therapy
Staphylococcus aureus
/ genetics
THP-1 Cells
Alpha-hemolysin
Binding interaction
Epigallocatechin gallate
MAPK
NLRP3 inflammasome
ROS
Journal
International immunopharmacology
ISSN: 1878-1705
Titre abrégé: Int Immunopharmacol
Pays: Netherlands
ID NLM: 100965259
Informations de publication
Date de publication:
Nov 2021
Nov 2021
Historique:
received:
21
06
2021
revised:
08
09
2021
accepted:
15
09
2021
pubmed:
26
9
2021
medline:
8
2
2022
entrez:
25
9
2021
Statut:
ppublish
Résumé
Alpha-hemolysin (Hla), the virulence factor secreted by Staphylococcus aureus (S. aureus), plays a critical role in infection and inflammation, which is a severe health burden worldwide. Therefore, it is necessary to develop a drug against Hla. Epigallocatechin gallate (EGCG), a polyphenol extracted from green tea, has excellent anti-inflammatory activity. In this study, we investigated the inhibitory effect of EGCG on Hla-induced NLRP3 inflammasome activation in vitro and in vivo and elucidated the potential molecular mechanism. We found that EGCG attenuated the hemolysis of Hla by inhibiting its secretion. Besides, EGCG significantly decreased overproduction of ROS and activation of MAPK signaling pathway induced by Hla, thereby markedly attenuating the expression of NLRP3 inflammasome-related proteins in THP-1 cells. Notably, EGCG could spontaneously bind to Hla with affinity constant of 1.71 × 10
Identifiants
pubmed: 34562843
pii: S1567-5769(21)00806-7
doi: 10.1016/j.intimp.2021.108170
pii:
doi:
Substances chimiques
Anti-Inflammatory Agents
0
Antioxidants
0
Bacterial Proteins
0
Hemolysin Proteins
0
Inflammasomes
0
NLR Family, Pyrin Domain-Containing 3 Protein
0
Nlrp3 protein, mouse
0
Reactive Oxygen Species
0
Catechin
8R1V1STN48
epigallocatechin gallate
BQM438CTEL
Mitogen-Activated Protein Kinases
EC 2.7.11.24
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
108170Informations de copyright
Copyright © 2021. Published by Elsevier B.V.