In Situ Quantification of Polyhydroxybutyrate in Photobioreactor Cultivations of
PAT
PHB
Synechocystis sp. PCC 6714
polyhydroxyalkanoates
process monitoring
ultrasound particle manipulation
Journal
Bioengineering (Basel, Switzerland)
ISSN: 2306-5354
Titre abrégé: Bioengineering (Basel)
Pays: Switzerland
ID NLM: 101676056
Informations de publication
Date de publication:
21 Sep 2021
21 Sep 2021
Historique:
received:
20
07
2021
revised:
10
09
2021
accepted:
17
09
2021
entrez:
25
9
2021
pubmed:
26
9
2021
medline:
26
9
2021
Statut:
epublish
Résumé
Polyhydroxybutyrate (PHB) is a very promising alternative to most petroleum-based plastics with the huge advantage of biodegradability. Biotechnological production processes utilizing cyanobacteria as sustainable source of PHB require fast in situ process analytical technology (PAT) tools for sophisticated process monitoring. Spectroscopic probes supported by ultrasound particle traps provide a powerful technology for in-line, nondestructive, and real-time process analytics in photobioreactors. This work shows the great potential of using ultrasound particle manipulation to improve spectroscopic attenuated total reflection Fourier-transformed mid-infrared (ATR-FTIR) spectra as a monitoring tool for PHB production processes in photobioreactors.
Identifiants
pubmed: 34562950
pii: bioengineering8090129
doi: 10.3390/bioengineering8090129
pmc: PMC8469707
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Interreg
ID : Plastocyan ATCZ260
Organisme : TU Wien
ID : bioactive
Références
Life (Basel). 2021 Jan 30;11(2):
pubmed: 33573287
Life (Basel). 2020 Apr 22;10(4):
pubmed: 32331427
Appl Environ Microbiol. 2000 Aug;66(8):3415-20
pubmed: 10919800
Bioresour Technol. 2019 Nov;292:121947
pubmed: 31466821
AMB Express. 2017 Dec;7(1):143
pubmed: 28687036
Anal Chim Acta. 2014 Jan 7;807:103-10
pubmed: 24356226
Bioengineering (Basel). 2017 Mar 28;4(2):
pubmed: 28952505
J Biotechnol. 2005 Apr 20;117(1):119-29
pubmed: 15831253
Anal Bioanal Chem. 2012 Sep;404(4):1211-37
pubmed: 22644146
J Microbiol Methods. 2021 Jul;186:106256
pubmed: 34082050
Photosynth Res. 2017 Apr;132(1):69-82
pubmed: 28108865
PLoS One. 2016 Jun 30;11(6):e0158168
pubmed: 27359097
Anal Chem. 2015 Feb 17;87(4):2314-20
pubmed: 25582569
Appl Spectrosc. 2016 Dec;70(12):1965-1973
pubmed: 27864445
Ultrasonics. 2010 Feb;50(2):240-6
pubmed: 19883932
Anal Chem. 2004 Nov 1;76(21):6353-8
pubmed: 15516127
J Microbiol Methods. 2016 Dec;131:166-171
pubmed: 27720900
Science. 2019 Sep 20;365(6459):
pubmed: 31604209
Bacteriol Rev. 1971 Jun;35(2):171-205
pubmed: 4998365
FEMS Microbiol Rev. 1992 Dec;9(2-4):317-21
pubmed: 1476776
Biomacromolecules. 2012 Jul 9;13(7):2118-27
pubmed: 22686500
Ultrasound Med Biol. 2013 Jun;39(6):1094-101
pubmed: 23562020
Sci Total Environ. 2020 Feb 1;702:134455
pubmed: 31733547
Bioengineering (Basel). 2019 Sep 19;6(3):
pubmed: 31546779