Extraction and LC-MS/MS Analysis of Ciguatoxins: A Semi-Targeted Approach Designed for Fish of Unknown Origin.
N2a
chromatography
ciguatoxins
food contaminants
food safety
mass spectrometry
validation
Journal
Toxins
ISSN: 2072-6651
Titre abrégé: Toxins (Basel)
Pays: Switzerland
ID NLM: 101530765
Informations de publication
Date de publication:
08 09 2021
08 09 2021
Historique:
received:
05
08
2021
revised:
31
08
2021
accepted:
07
09
2021
entrez:
26
9
2021
pubmed:
27
9
2021
medline:
8
2
2022
Statut:
epublish
Résumé
Ciguatoxins (CTXs) are polyether marine biotoxins that can cause ciguatera poisoning (CP) after the consumption of fish or invertebrates containing sub ppb levels; concentrations that present a challenge for current extraction and analysis methods. Here, a newly developed and (partly) validated single-day extraction protocol is presented. First, the fish sample is broken-down by enzymatic digestion, followed by extraction and extract clean-up by defatting and two solid-phase extractions. Final extracts were investigated using two different CTX-analysis methods; an in vitro cytotoxicity assay (N2a-assay) and by LC-MS/MS. Validation was performed for both fillet and freeze-dried samples of snapper, parrotfish, and grouper spiked with CTX1B, 52
Identifiants
pubmed: 34564634
pii: toxins13090630
doi: 10.3390/toxins13090630
pmc: PMC8473320
pii:
doi:
Substances chimiques
Ciguatoxins
11050-21-8
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
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