Relative frequency of hepatitis B virus, human papilloma virus, Epstein-Barr virus, and herpes simplex viruses in the semen of fertile and infertile men in Shiraz, Iran: A cross-sectional study.
Epstein-Barr virus
Hepatitis B virus
Herpes simplex viruses.
Human papilloma virus
Male infertility
Journal
International journal of reproductive biomedicine
ISSN: 2476-4108
Titre abrégé: Int J Reprod Biomed
Pays: Iran
ID NLM: 101679102
Informations de publication
Date de publication:
Aug 2021
Aug 2021
Historique:
received:
25
03
2020
revised:
01
11
2020
accepted:
16
12
2020
entrez:
27
9
2021
pubmed:
28
9
2021
medline:
28
9
2021
Statut:
epublish
Résumé
About 8-12% of couples on reproductive age suffers from infertility worldwide. Since 1993, the role of genital tract infections by microbes, including viruses that can infect the sperm, in human infertility has been proposed. To investigate the frequency of hepatitis B virus (HBV), human papilloma virus (HPV), Epstein-Barr virus (EBV), and herpes simplex virus (HSV) infection in the semen of fertile and infertile men referred to the Mother and Child Hospital, Shiraz, Iran. In this cross-sectional study, 350 men including 200 infertile and 150 fertile men were included. All semen samples were allowed to liquefy, followed by the assessment of sperm parameters. DNA was extracted using a DNA extraction kit (CinaGene, Tehran, Iran) according to the manufacturer's instructions. Detection of HBV, HPV, EBV, and HSV1/2 was done by the PCR method. The mean age of the participants was 36 The results of this study indicated that HBV, HPV, EBV, and HSV might not be involved in men's infertility. Further studies are recommended for clarifying the role of these viruses in infertility.
Sections du résumé
BACKGROUND
BACKGROUND
About 8-12% of couples on reproductive age suffers from infertility worldwide. Since 1993, the role of genital tract infections by microbes, including viruses that can infect the sperm, in human infertility has been proposed.
OBJECTIVE
OBJECTIVE
To investigate the frequency of hepatitis B virus (HBV), human papilloma virus (HPV), Epstein-Barr virus (EBV), and herpes simplex virus (HSV) infection in the semen of fertile and infertile men referred to the Mother and Child Hospital, Shiraz, Iran.
MATERIALS AND METHODS
METHODS
In this cross-sectional study, 350 men including 200 infertile and 150 fertile men were included. All semen samples were allowed to liquefy, followed by the assessment of sperm parameters. DNA was extracted using a DNA extraction kit (CinaGene, Tehran, Iran) according to the manufacturer's instructions. Detection of HBV, HPV, EBV, and HSV1/2 was done by the PCR method.
RESULTS
RESULTS
The mean age of the participants was 36
CONCLUSION
CONCLUSIONS
The results of this study indicated that HBV, HPV, EBV, and HSV might not be involved in men's infertility. Further studies are recommended for clarifying the role of these viruses in infertility.
Identifiants
pubmed: 34568730
doi: 10.18502/ijrm.v19i8.9617
pmc: PMC8458917
doi:
Types de publication
Journal Article
Langues
eng
Pagination
699-706Informations de copyright
Copyright © 2021 Afrakhteh et al.
Déclaration de conflit d'intérêts
All authors declare no conflict of interest.
Références
Andrologia. 2008 Apr;40(2):72-5
pubmed: 18336453
Andrologia. 2018 Dec;50(11):e13140
pubmed: 30569651
Int J Fertil Steril. 2019 Jan;12(4):306-309
pubmed: 30291691
Int J Reprod Biomed. 2018 Sep;16(9):595-600
pubmed: 30643867
J Reprod Immunol. 2013 Nov;100(1):20-9
pubmed: 23668923
Zhonghua Nan Ke Xue. 2017 May;23(5):431-435
pubmed: 29717834
J Biomed Res. 2013 Jan;27(1):56-61
pubmed: 23554795
Hum Fertil (Camb). 2019 Jun;22(2):133-138
pubmed: 29688091
Urol Clin North Am. 2014 Feb;41(1):195-204
pubmed: 24286777
Fertil Steril. 2007 May;87(5):1087-97
pubmed: 17433312
Urologe A. 2016 Jul;55(7):883-9
pubmed: 27335094
Fertil Steril. 2014 Dec;102(6):1677-84
pubmed: 25439807
Clin Biochem. 2018 Dec;62:2-10
pubmed: 29555319
BMC Infect Dis. 2017 Nov 9;17(1):714
pubmed: 29121862
Fertil Steril. 2010 Oct;94(5):1723-7
pubmed: 20056213
Bull World Health Organ. 2010 Dec 1;88(12):881-2
pubmed: 21124709
Fertil Steril. 2009 Jun;91(6):2487-94
pubmed: 18565516
Andrology. 2015 Mar;3(2):163-73
pubmed: 25270519
Am J Reprod Immunol. 2016 Sep;76(3):183-5
pubmed: 27381166
Fertil Steril. 2003 Jun;79 Suppl 3:1566-70
pubmed: 12801561
Int J Reprod Biomed. 2019 Sep 03;17(8):551-556
pubmed: 31583372
Urology. 2018 Mar;113:52-58
pubmed: 29287977
Gastroenterol Hepatol Bed Bench. 2019 Winter;12(1):38-45
pubmed: 30949318
Reprod Biol Endocrinol. 2015 Apr 26;13:37
pubmed: 25928197
Int J STD AIDS. 2004 Nov;15(11):740-3
pubmed: 15537460
Infect Disord Drug Targets. 2020;20(2):198-202
pubmed: 30474539
Biomed Res Int. 2014;2014:230263
pubmed: 24783196
Iran J Immunol. 2015 Mar;12(1):27-34
pubmed: 25784095
Med J Islam Repub Iran. 2015 Oct 04;29:272
pubmed: 26793663
Pol J Microbiol. 2018 Mar 9;67(1):73-79
pubmed: 30015427