Recent advancements in mass spectrometry for higher order structure characterization of protein therapeutics.
FPOP
HDX
Higher order structure
Mass spectrometry
Native MS
Protein therapeutics
Journal
Drug discovery today
ISSN: 1878-5832
Titre abrégé: Drug Discov Today
Pays: England
ID NLM: 9604391
Informations de publication
Date de publication:
01 2022
01 2022
Historique:
received:
02
04
2021
revised:
30
07
2021
accepted:
20
09
2021
pubmed:
28
9
2021
medline:
16
3
2022
entrez:
27
9
2021
Statut:
ppublish
Résumé
Molecular characterization of higher order structure (HOS) in protein therapeutics is crucial to the selection of candidate molecules, understanding of structure-function relationships, formulation development, stability assessment, and comparability studies. Recent advances in mass spectrometry (MS), including native MS, hydrogen/deuterium exchange (HDX)-MS, and fast photochemical oxidation of proteins (FPOP) coupled with MS, have provided orthogonal ways to characterize HOS of protein therapeutics. In this review, we present the utility of native MS, HDX-MS and FPOP-MS in protein therapeutics discovery and development, with a focus on epitope mapping, aggregation assessment, and comparability studies. We also discuss future trends in the application of these MS methods to HOS characterization.
Identifiants
pubmed: 34571276
pii: S1359-6446(21)00404-9
doi: 10.1016/j.drudis.2021.09.010
pii:
doi:
Substances chimiques
Proteins
0
Types de publication
Journal Article
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
196-206Informations de copyright
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