A Universal Approach to Analyzing Transmission Electron Microscopy with ImageJ.
ImageJ
Mitochondria Endoplasmic Reticulum Contacts (MERCs)
TEM analysis
TEM quantification
cristae
image analysis
image processing
mitochondria
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
24 08 2021
24 08 2021
Historique:
received:
17
06
2021
revised:
18
08
2021
accepted:
19
08
2021
entrez:
28
9
2021
pubmed:
29
9
2021
medline:
20
11
2021
Statut:
epublish
Résumé
Transmission electron microscopy (TEM) is widely used as an imaging modality to provide high-resolution details of subcellular components within cells and tissues. Mitochondria and endoplasmic reticulum (ER) are organelles of particular interest to those investigating metabolic disorders. A straightforward method for quantifying and characterizing particular aspects of these organelles would be a useful tool. In this protocol, we outline how to accurately assess the morphology of these important subcellular structures using open source software
Identifiants
pubmed: 34571826
pii: cells10092177
doi: 10.3390/cells10092177
pmc: PMC8465115
pii:
doi:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NHLBI NIH HHS
ID : T32 HL007121
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL108379
Pays : United States
Organisme : NIDDK NIH HHS
ID : R01 DK092065
Pays : United States
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