PTX-3 Secreted by Intra-Articular-Injected SMUP-Cells Reduces Pain in an Osteoarthritis Rat Model.
Animals
C-Reactive Protein
/ metabolism
Cytokines
/ metabolism
Disease Models, Animal
Inflammation
/ etiology
Injections, Intra-Articular
Iodoacetic Acid
/ toxicity
Macrophage Activation
/ immunology
Macrophages
/ immunology
Male
Mesenchymal Stem Cell Transplantation
/ methods
Mesenchymal Stem Cells
/ cytology
Osteoarthritis
/ chemically induced
Osteocytes
/ cytology
Pain
/ etiology
Rats
Rats, Sprague-Dawley
Serum Amyloid P-Component
/ metabolism
PTX-3
SMUP-Cells
bioreactor
cell therapy
macrophage polarization
mesenchymal stem cells
osteoarthritis
pain relief
Journal
Cells
ISSN: 2073-4409
Titre abrégé: Cells
Pays: Switzerland
ID NLM: 101600052
Informations de publication
Date de publication:
14 09 2021
14 09 2021
Historique:
received:
08
08
2021
revised:
10
09
2021
accepted:
11
09
2021
entrez:
28
9
2021
pubmed:
29
9
2021
medline:
16
11
2021
Statut:
epublish
Résumé
Mesenchymal stem cells (MSCs) are accessible, abundantly available, and capable of regenerating; they have the potential to be developed as therapeutic agents for diseases. However, concerns remain in their further application. In this study, we developed a SMall cell+Ultra Potent+Scale UP cell (SMUP-Cell) platform to improve whole-cell processing, including manufacturing bioreactors and xeno-free solutions for commercialization. To confirm the superiority of SMUP-Cell improvements, we demonstrated that a molecule secreted by SMUP-Cells is capable of polarizing inflammatory macrophages (M1) into their anti-inflammatory phenotype (M2) at the site of injury in a pain-associated osteoarthritis (OA) model. Lipopolysaccharide-stimulated macrophages co-cultured with SMUP-Cells expressed low levels of M1-phenotype markers (CD11b, tumor necrosis factor-α, interleukin-1α, and interleukin-6), but high levels of M2 markers (CD163 and arginase-1). To identify the paracrine action underlying the anti-inflammatory effect of SMUP-Cells, we employed a cytokine array and detected increased levels of pentraxin-related protein-3 (PTX-3). Additionally,
Identifiants
pubmed: 34572070
pii: cells10092420
doi: 10.3390/cells10092420
pmc: PMC8466059
pii:
doi:
Substances chimiques
Cytokines
0
Serum Amyloid P-Component
0
PTX3 protein
148591-49-5
C-Reactive Protein
9007-41-4
Iodoacetic Acid
WF5188V710
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Références
Sci Rep. 2019 Mar 18;9(1):4696
pubmed: 30886197
Clin Transl Med. 2017 Oct 10;6(1):38
pubmed: 29019171
World J Stem Cells. 2020 Dec 26;12(12):1511-1528
pubmed: 33505598
Biochem Biophys Rep. 2016 Jan 14;5:290-295
pubmed: 28955836
J Headache Pain. 2017 Dec;18(1):34
pubmed: 28303458
Cytotherapy. 2006;8(4):315-7
pubmed: 16923606
Intensive Care Med Exp. 2017 Dec;5(1):13
pubmed: 28265979
Cell Stem Cell. 2010 Feb 5;6(2):103-15
pubmed: 20144784
Stem Cells Int. 2018 Sep 12;2018:9415367
pubmed: 30275839
Stem Cell Res Ther. 2019 Mar 18;10(1):99
pubmed: 30885254
Stem Cell Res Ther. 2016 Sep 09;7(1):129
pubmed: 27612636
Appl Microbiol Biotechnol. 2018 May;102(9):3981-3994
pubmed: 29564526
Biomed Res Int. 2013;2013:284873
pubmed: 24069595
Osteoarthritis Cartilage. 2013 Sep;21(9):1145-53
pubmed: 23973124
Pain. 2019 Apr;160(4):895-907
pubmed: 30585984
Leukemia. 2018 Dec;32(12):2672-2684
pubmed: 29789652
Microb Cell Fact. 2019 Sep 4;18(1):150
pubmed: 31484570
Stem Cells Int. 2020 Apr 28;2020:5924983
pubmed: 32399043
Cells. 2021 Jan 03;10(1):
pubmed: 33401590
Front Immunol. 2020 Feb 11;11:111
pubmed: 32117263
Bone Res. 2017 Jan 17;5:16044
pubmed: 28149655
Front Cell Dev Biol. 2020 Jun 03;8:364
pubmed: 32582691
Exp Anim. 2018 May 10;67(2):271-280
pubmed: 29311503
F1000Prime Rep. 2014 Mar 03;6:13
pubmed: 24669294
Front Cell Dev Biol. 2016 Jun 10;4:56
pubmed: 27376065
J Invest Dermatol. 2016 Jan;136(1):293-300
pubmed: 26763449
Ther Adv Musculoskelet Dis. 2019 Jul 23;11:1759720X19864492
pubmed: 31384314
Biomed Res Int. 2013;2013:762132
pubmed: 24000327
Clin Calcium. 2013 Dec;23(12):1705-13
pubmed: 24292524
Int J Mol Sci. 2018 Jan 09;19(1):
pubmed: 29315262
Stem Cells Transl Med. 2016 Apr;5(4):427-39
pubmed: 26941359
J Neurosci. 2014 Oct 15;34(42):14055-68
pubmed: 25319702
Sci Rep. 2016 Aug 02;6:30881
pubmed: 27481042
Exp Mol Med. 2019 Jul 9;51(7):1-14
pubmed: 31285429
J Neurosci Res. 2015 Dec;93(12):1814-25
pubmed: 26332684
Arch Orthop Trauma Surg. 2020 Apr;140(4):503-509
pubmed: 31980879
NPJ Regen Med. 2019 Dec 2;4:22
pubmed: 31815001
Transfusion. 2018 Oct;58(10):2374-2382
pubmed: 30203447
Nat Rev Rheumatol. 2016 Jul;12(7):412-20
pubmed: 27192932
Stem Cells Int. 2018 Jul 11;2018:6545071
pubmed: 30123291
J Biotechnol. 2017 Apr 20;248:87-98
pubmed: 28174039
F1000Res. 2020 May 4;9:
pubmed: 32419923
Stem Cells Int. 2020 Jan 23;2020:1802976
pubmed: 32399038
Tissue Eng Regen Med. 2019 Jan 23;16(4):357-363
pubmed: 31413940
Int J Mol Sci. 2013 Sep 03;14(9):17986-8001
pubmed: 24005862
Int J Mol Sci. 2020 Feb 25;21(5):
pubmed: 32106481
Cytotherapy. 2019 Mar;21(3):289-306
pubmed: 30528726
Sci Rep. 2018 Jan 10;8(1):354
pubmed: 29321508
PLoS One. 2013;8(3):e60086
pubmed: 23533666