Severe Traumatic Injury Induces Phenotypic and Functional Changes of Neutrophils and Monocytes.
CD14
CD16
CD62L
PMA
fMLP
phagocytosis
reactive oxygen species
traumatic injury
Journal
Journal of clinical medicine
ISSN: 2077-0383
Titre abrégé: J Clin Med
Pays: Switzerland
ID NLM: 101606588
Informations de publication
Date de publication:
14 Sep 2021
14 Sep 2021
Historique:
received:
31
08
2021
revised:
09
09
2021
accepted:
11
09
2021
entrez:
28
9
2021
pubmed:
29
9
2021
medline:
29
9
2021
Statut:
epublish
Résumé
Severe traumatic injury has been associated with high susceptibility for the development of secondary complications caused by dysbalanced immune response. As the first line of the cellular immune response, neutrophils and monocytes recruited to the site of tissue damage and/or infection, are divided into three different subsets according to their CD16/CD62L and CD16/CD14 expression, respectively. Their differential functions have not yet been clearly understood. Thus, we evaluated the phenotypic changes of neutrophil and monocyte subsets among their functionality regarding oxidative burst and the phagocytic capacity in severely traumatized patients. Peripheral blood was withdrawn from severely injured trauma patients (TP; In TP, the counts of immature neutrophils were significantly increased vs. HV. The numbers of mature and CD62L Neutrophils and monocytes display a phenotypic shift following severe injury. The increased functional abnormalities of certain subsets may contribute to the dysbalanced immune response and attenuate the antimicrobial function and thus, may represent a potential therapeutic target. Further studies on isolated subsets are necessary for evaluation of their physiological role after severe traumatic injury.
Sections du résumé
BACKGROUND
BACKGROUND
Severe traumatic injury has been associated with high susceptibility for the development of secondary complications caused by dysbalanced immune response. As the first line of the cellular immune response, neutrophils and monocytes recruited to the site of tissue damage and/or infection, are divided into three different subsets according to their CD16/CD62L and CD16/CD14 expression, respectively. Their differential functions have not yet been clearly understood. Thus, we evaluated the phenotypic changes of neutrophil and monocyte subsets among their functionality regarding oxidative burst and the phagocytic capacity in severely traumatized patients.
METHODS
METHODS
Peripheral blood was withdrawn from severely injured trauma patients (TP;
RESULTS
RESULTS
In TP, the counts of immature neutrophils were significantly increased vs. HV. The numbers of mature and CD62L
CONCLUSIONS
CONCLUSIONS
Neutrophils and monocytes display a phenotypic shift following severe injury. The increased functional abnormalities of certain subsets may contribute to the dysbalanced immune response and attenuate the antimicrobial function and thus, may represent a potential therapeutic target. Further studies on isolated subsets are necessary for evaluation of their physiological role after severe traumatic injury.
Identifiants
pubmed: 34575249
pii: jcm10184139
doi: 10.3390/jcm10184139
pmc: PMC8467869
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : Deutsche Forschungsgemeinschaft
ID : RE 3304/8-1
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