Spatiotemporal Correlation Spectroscopy Reveals a Protective Effect of Peptide-Based GLP-1 Receptor Agonism against Lipotoxicity on Insulin Granule Dynamics in Primary Human β-Cells.
GLP-1 receptor agonism
fluorescence
iMSD
insulin secretory granule dynamics
pancreatic islets
syncollin
β-cells
Journal
Pharmaceutics
ISSN: 1999-4923
Titre abrégé: Pharmaceutics
Pays: Switzerland
ID NLM: 101534003
Informations de publication
Date de publication:
03 Sep 2021
03 Sep 2021
Historique:
received:
16
07
2021
revised:
18
08
2021
accepted:
02
09
2021
entrez:
28
9
2021
pubmed:
29
9
2021
medline:
29
9
2021
Statut:
epublish
Résumé
Glucagon-like peptide-1 receptor (GLP-1R) agonists are being used for the treatment of type 2 diabetes (T2D) and may have beneficial effects on the pancreatic β-cells. Here, we evaluated the effects of GLP-1R agonism on insulin secretory granule (ISG) dynamics in primary β-cells isolated from human islets exposed to palmitate-induced lipotoxic stress. Islets cells were exposed for 48 h to 0.5 mM palmitate (hereafter, 'Palm') with or without the addition of a GLP-1 agonist, namely 10 nM exendin-4 (hereafter, 'Ex-4'). Dissociated cells were first transfected with syncollin-EGFP in order to fluorescently mark the ISGs. Then, by applying a recently established spatiotemporal correlation spectroscopy technique, the average structural (i.e., size) and dynamic (i.e., the local diffusivity and mode of motion) properties of ISGs are extracted from a calculated imaging-derived Mean Square Displacement (
Identifiants
pubmed: 34575477
pii: pharmaceutics13091403
doi: 10.3390/pharmaceutics13091403
pmc: PMC8464798
pii:
doi:
Types de publication
Journal Article
Langues
eng
Subventions
Organisme : H2020 European Research Council
ID : 866127
Références
Proc Natl Acad Sci U S A. 2004 Jun 22;101(25):9266-71
pubmed: 15197259
PLoS One. 2014 Oct 13;9(10):e109082
pubmed: 25310693
Diabetes Spectr. 2017 Aug;30(3):202-210
pubmed: 28848315
J Clin Endocrinol Metab. 2001 Sep;86(9):4047-58
pubmed: 11549624
Biochem J. 2004 Jul 1;381(Pt 1):13-8
pubmed: 15128287
Int J Mol Sci. 2017 Dec 22;19(1):
pubmed: 29271910
Diabetologia. 2003 Jan;46(1):3-19
pubmed: 12637977
Biomolecules. 2021 Feb 19;11(2):
pubmed: 33669901
Diabetes. 2017 May;66(5):1272-1285
pubmed: 28232493
Exp Mol Med. 2017 Feb 3;49(2):e291
pubmed: 28154371
Curr Diab Rep. 2014 Jun;14(6):492
pubmed: 24740729
Diabetologia. 2012 Apr;55(4):1013-23
pubmed: 22252472
Diabetologia. 2014 Feb;57(2):362-5
pubmed: 24233056
Endocr Rev. 2007 Apr;28(2):187-218
pubmed: 17353295
Sci Rep. 2017 Nov 1;7(1):14836
pubmed: 29093485
Sci Data. 2018 Sep 18;5:180191
pubmed: 30226484
Biochim Biophys Acta. 2016 Sep;1861(9 Pt A):1151-1160
pubmed: 27344025
J Clin Invest. 2011 Jun;121(6):2118-25
pubmed: 21633180
Acta Biomater. 2016 Sep 15;42:189-198
pubmed: 27449340
Nature. 2018 Aug;560(7716):107-111
pubmed: 30022165
Metabolism. 2020 Sep;110:154304
pubmed: 32599081
Traffic. 2012 Nov;13(11):1466-80
pubmed: 22889194
J Mol Biol. 2020 Mar 6;432(5):1347-1366
pubmed: 31446075
Diabetes. 2001 Aug;50(8):1771-7
pubmed: 11473037
Front Endocrinol (Lausanne). 2018 Jul 16;9:384
pubmed: 30061862
Diabetes. 2009 Dec;58(12):2851-62
pubmed: 19720788
Diabetologia. 2013 Mar;56(3):497-507
pubmed: 23242133
J Mol Biol. 2020 Mar 6;432(5):1514-1534
pubmed: 31628942
Cell Rep. 2020 Dec 1;33(9):108466
pubmed: 33264613
Diabetes. 2020 Mar;69(3):279-290
pubmed: 32079704
Proc Natl Acad Sci U S A. 2013 Jul 23;110(30):12307-12
pubmed: 23836651
Proc Natl Acad Sci U S A. 2020 Feb 4;117(5):2484-2495
pubmed: 31964806
Mol Metab. 2021 Apr;46:101090
pubmed: 32987188
Diabetes. 2012 Jul;61(7):1726-33
pubmed: 22492527
Nat Rev Cancer. 2008 Nov;8(11):835-50
pubmed: 18948996
Cell Rep. 2020 Jul 14;32(2):107904
pubmed: 32668245
Biophys J. 2016 Aug 23;111(4):679-685
pubmed: 27558712
Trends Endocrinol Metab. 2010 Oct;21(10):599-609
pubmed: 20609596
Mediators Inflamm. 2016;2016:3094642
pubmed: 27110066
BMC Genomics. 2020 Aug 26;21(1):590
pubmed: 32847508
Sci Rep. 2019 Feb 27;9(1):2890
pubmed: 30814595
Proc Natl Acad Sci U S A. 2013 Mar 26;110(13):4911-6
pubmed: 23479621
Proc Natl Acad Sci U S A. 2015 Feb 17;112(7):E667-76
pubmed: 25646459
Biochem Biophys Res Commun. 2018 Sep 18;503(4):2704-2709
pubmed: 30100062
Int J Mol Sci. 2020 May 11;21(9):
pubmed: 32403391
Front Endocrinol (Lausanne). 2021 Mar 09;12:613964
pubmed: 33767668