Early Embryo Exposure to Assisted Reproductive Manipulation Induced Subtle Changes in Liver Epigenetics with No Apparent Negative Health Consequences in Rabbit.
Animals
DNA Methylation
Embryo Transfer
Embryo, Mammalian
/ metabolism
Epigenesis, Genetic
Epigenomics
Female
Genome
Humans
Lipid Metabolism
Liver
/ embryology
Male
Metabolome
Pregnancy
Pregnancy, Animal
Proteome
Proteomics
/ methods
Rabbits
Reproduction
Reproductive Techniques, Assisted
Steroids
/ biosynthesis
Vitrification
DNA methylation
assisted reproductive technologies
metabolome
proteome
steroid biosynthesis
stress
vitrification
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
08 Sep 2021
08 Sep 2021
Historique:
received:
14
06
2021
revised:
30
08
2021
accepted:
06
09
2021
entrez:
28
9
2021
pubmed:
29
9
2021
medline:
16
11
2021
Statut:
epublish
Résumé
Embryo manipulation is a requisite step in assisted reproductive technology (ART). Therefore, it is of great necessity to appraise the safety of ART and investigate the long-term effect, including lipid metabolism, on ART-conceived offspring. Augmenting our ART rabbit model to investigate lipid metabolic outcomes in offspring longitudinally, we detected variations in hepatic DNA methylation ART offspring in the F3 generation for embryonic exposure (multiple ovulation, vitrification and embryo transfer). Through adult liver metabolomics and proteomics, we identified changes mainly related to lipid metabolism (e.g., polyunsaturated fatty acids, steroids, steroid hormone). We also found that DNA methylation analysis was linked to changes in lipid metabolism and apoptosis genes. Nevertheless, these differences did not apparently alter the general health status. Thus, our findings suggest that ART is likely to be a player in embryo epigenetic events related to hepatic homeostasis alteration in adulthood.
Identifiants
pubmed: 34575877
pii: ijms22189716
doi: 10.3390/ijms22189716
pmc: PMC8467347
pii:
doi:
Substances chimiques
Proteome
0
Steroids
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Spanish National Plan for Scientific and Technical Research and Innovation
ID : AGL2014-53405-C2-1-P
Organisme : Conselleria d'Educació, Investigació, Cultura i Esport
ID : Prometeo II 2014/036
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