Sensitivity of Rapid Antigen Testing and RT-PCR Performed on Nasopharyngeal Swabs versus Saliva Samples in COVID-19 Hospitalized Patients: Results of a Prospective Comparative Trial (RESTART).

RT-PCR SARS-CoV-2 diagnosis rapid antigen testing saliva viral transport medium

Journal

Microorganisms
ISSN: 2076-2607
Titre abrégé: Microorganisms
Pays: Switzerland
ID NLM: 101625893

Informations de publication

Date de publication:
09 Sep 2021
Historique:
received: 13 08 2021
revised: 31 08 2021
accepted: 07 09 2021
entrez: 28 9 2021
pubmed: 29 9 2021
medline: 29 9 2021
Statut: epublish

Résumé

Saliva sampling could serve as an alternative non-invasive sample for SARS-CoV-2 diagnosis while rapid antigen tests (RATs) might help to mitigate the shortage of reagents sporadically encountered with RT-PCR. Thus, in the RESTART study we compared antigen and RT-PCR testing methods on nasopharyngeal (NP) swabs and salivary samples. We conducted a prospective observational study among COVID-19 hospitalized patients between 10 December 2020 and 1 February 2021. Paired saliva and NP samples were investigated by RT-PCR (Cobas 6800, Roche-Switzerland, Basel, Switzerland) and by two rapid antigen tests: One Step Immunoassay Exdia

Identifiants

pubmed: 34576805
pii: microorganisms9091910
doi: 10.3390/microorganisms9091910
pmc: PMC8464722
pii:
doi:

Types de publication

Journal Article

Langues

eng

Références

J Clin Microbiol. 2008 Jul;46(7):2374-6
pubmed: 18463214
Travel Med Infect Dis. 2021 Jan-Feb;39:101942
pubmed: 33278609
Clin Microbiol Infect. 2020 Dec;26(12):1711-1713
pubmed: 32688069
Pathog Dis. 2020 Nov 11;78(8):
pubmed: 33030200
Clin Microbiol Infect. 2020 Sep;26(9):1178-1182
pubmed: 32593741
BMC Microbiol. 2021 Jan 22;21(1):31
pubmed: 33482729
Microbes Infect. 2020 Nov - Dec;22(10):617-621
pubmed: 32911086
Clin Infect Dis. 2021 Mar 15;72(6):1064-1066
pubmed: 32584972
Clin Microbiol Infect. 2021 Apr;27(4):656-657
pubmed: 33160035
Microorganisms. 2021 Apr 10;9(4):
pubmed: 33920307
N Engl J Med. 2020 May 28;382(22):e76
pubmed: 32302471
N Engl J Med. 2020 Sep 24;383(13):1283-1286
pubmed: 32857487
Sci Rep. 2021 Feb 4;11(1):3134
pubmed: 33542443
Sci Transl Med. 2021 Jan 20;13(577):
pubmed: 33288662
PLoS One. 2021 Mar 31;16(3):e0248921
pubmed: 33788882
Clin Microbiol Infect. 2021 Feb;27(2):285.e1-285.e4
pubmed: 32422408
Clin Infect Dis. 2021 Jan 22;:
pubmed: 33480973
Cochrane Database Syst Rev. 2011 Dec 07;(12):CD008934
pubmed: 22161442
J Clin Microbiol. 2020 Jul 23;58(8):
pubmed: 32341141
Eur J Clin Microbiol Infect Dis. 2021 Jan;40(1):193-195
pubmed: 32666481
J Infect. 2020 Jul;81(1):e45-e50
pubmed: 32298676
JAMA Intern Med. 2021 Mar 1;181(3):353-360
pubmed: 33449069
Pathology. 2020 Dec;52(7):811-814
pubmed: 33250079

Auteurs

Antonios Kritikos (A)

Institute of Microbiology, Lausanne University Hospital and University of Lausanne, 1011 Lausanne, Switzerland.

Giorgia Caruana (G)

Institute of Microbiology, Lausanne University Hospital and University of Lausanne, 1011 Lausanne, Switzerland.

René Brouillet (R)

Institute of Microbiology, Lausanne University Hospital and University of Lausanne, 1011 Lausanne, Switzerland.

John-Paul Miroz (JP)

Intensive Care Unit, Lausanne University Hospital and University of Lausanne, 1011 Lausanne, Switzerland.

Samia Abed-Maillard (S)

Intensive Care Unit, Lausanne University Hospital and University of Lausanne, 1011 Lausanne, Switzerland.

Geraldine Stieger (G)

Intensive Care Unit, Lausanne University Hospital and University of Lausanne, 1011 Lausanne, Switzerland.

Onya Opota (O)

Institute of Microbiology, Lausanne University Hospital and University of Lausanne, 1011 Lausanne, Switzerland.

Antony Croxatto (A)

Institute of Microbiology, Lausanne University Hospital and University of Lausanne, 1011 Lausanne, Switzerland.

Peter Vollenweider (P)

Service of Internal Medicine, Lausanne University Hospital and University of Lausanne, 1011 Lausanne, Switzerland.

Pierre-Alexandre Bart (PA)

Service of Internal Medicine, Lausanne University Hospital and University of Lausanne, 1011 Lausanne, Switzerland.

Jean-Daniel Chiche (JD)

Intensive Care Unit, Lausanne University Hospital and University of Lausanne, 1011 Lausanne, Switzerland.

Gilbert Greub (G)

Institute of Microbiology, Lausanne University Hospital and University of Lausanne, 1011 Lausanne, Switzerland.

Classifications MeSH