Lung Extracellular Matrix as a Platform for Lung Organ Bioengineering: Design and Development of Tissue Engineered Lung.

Decellularization Lung matrices Lung stem cells Recellularization Scaffold

Journal

Advances in experimental medicine and biology
ISSN: 0065-2598
Titre abrégé: Adv Exp Med Biol
Pays: United States
ID NLM: 0121103

Informations de publication

Date de publication:
2021
Historique:
entrez: 28 9 2021
pubmed: 29 9 2021
medline: 1 10 2021
Statut: ppublish

Résumé

Since lung tissue is not able to be reconstructed after substantial injury, lung transplantation often is the only alternative for treatment. Antibiotic-resistant organisms that remain in donor lungs causing infection in the immunosuppressed recipient are among the complications following transplantation. Development of strategies for whole lung regeneration is a pleasing choice particularly in patients with end-stage lung diseases. Reconstruction of lung tissue in vitro for transplantation received increased attention which could deal with the shortage of donor organs. Recent advancements in the field of tissue engineering and regenerative medicine have paved the road for beneficial alternative therapies. Our group has extensive experience with regard to the structure of the lung tissue, which makes us to our decision to continue with the preparation of lung, with the aim of developing a new ECM scaffold. Herein, we aim to review the state-of-art and the tissue engineering and regenerative medicine technology highlighting the major achievements toward the production of a bioengineered lung obtained decellularization and recellularization techniques. We have strong hopes that recent developments in the engineering of lung will lead to similar breakthroughs in the engineering of distal lung components in the future.

Identifiants

pubmed: 34582012
doi: 10.1007/978-3-030-82735-9_4
doi:

Types de publication

Journal Article Review

Langues

eng

Sous-ensembles de citation

IM

Pagination

35-46

Informations de copyright

© 2021. Springer Nature Switzerland AG.

Références

Ali NN, Edgar AJ, Samadikuchaksaraei A, Timson CM, Romanska HM, Polak JM et al (2002) Derivation of type II alveolar epithelial cells from murine embryonic stem cells. Tissue Eng 8(4):541–550
pubmed: 12201994 doi: 10.1089/107632702760240463 pmcid: 12201994
Azargoon A, Negahdari B (2018) Lung regeneration using amniotic fluid mesenchymal stem cells. Artif Cells, Nanomed, Biotechnol 46(3):447–451
doi: 10.1080/21691401.2017.1337023
Badylak SF, Gilbert TW (eds) (2008) Immune response to biologic scaffold materials. Seminars in immunology. Elsevier
Badylak SF, Taylor D, Uygun K (2011) Whole-organ tissue engineering: decellularization and recellularization of three-dimensional matrix scaffolds. Annu Rev Biomed Eng 13:27–53
pubmed: 21417722 doi: 10.1146/annurev-bioeng-071910-124743
Barkauskas CE, Cronce MJ, Rackley CR, Bowie EJ, Keene DR, Stripp BR et al (2013) Type 2 alveolar cells are stem cells in adult lung. J Clin Investig 123(7):3025–3036
pubmed: 23921127 pmcid: 3696553 doi: 10.1172/JCI68782
Booth AJ, Hadley R, Cornett AM, Dreffs AA, Matthes SA, Tsui JL et al (2012) Acellular normal and fibrotic human lung matrices as a culture system for in vitro investigation. Am J Respir Crit Care Med 186(9):866–876
pubmed: 22936357 pmcid: 3530219 doi: 10.1164/rccm.201204-0754OC
Brown BN, Valentin JE, Stewart-Akers AM, McCabe GP, Badylak SF (2009) Macrophage phenotype and remodeling outcomes in response to biologic scaffolds with and without a cellular component. Biomaterials 30(8):1482–1491
pubmed: 19121538 pmcid: 2805023 doi: 10.1016/j.biomaterials.2008.11.040
Burgstaller G, Oehrle B, Gerckens M, White ES, Schiller HB, Eickelberg O (2017) The instructive extracellular matrix of the lung: basic composition and alterations in chronic lung disease. Eur Respir J 50(1):1601805
pubmed: 28679607 doi: 10.1183/13993003.01805-2016 pmcid: 28679607
Calle EA, Leiby KL, Raredon MB, Niklason LE (2017) Lung regeneration: steps toward clinical implementation and use. Curr Opin Anaesthesiol 30(1):23
pubmed: 27922848 pmcid: 5283945 doi: 10.1097/ACO.0000000000000425
Chang YS, Oh W, Choi SJ, Sung DK, Kim SY, Choi EY et al (2009) Human umbilical cord blood-derived mesenchymal stem cells attenuate hyperoxia-induced lung injury in neonatal rats. Cell Transplant 18(8):869–886
pubmed: 19500472 doi: 10.3727/096368909X471189 pmcid: 19500472
Coraux C, Nawrocki-Raby B, Hinnrasky J, Kileztky C, Gaillard D, Dani C et al (2005) Embryonic stem cells generate airway epithelial tissue. Am J Respir Cell Mol Biol 32(2):87–92
pubmed: 15576671 doi: 10.1165/rcmb.2004-0079RC pmcid: 15576671
Cortiella J, Nichols JE, Kojima K, Bonassar LJ, Dargon P, Roy AK et al (2006) Tissue-engineered lung: an in vivo and in vitro comparison of polyglycolic acid and pluronic F-127 hydrogel/somatic lung progenitor cell constructs to support tissue growth. Tissue Eng 12(5):1213–1225
pubmed: 16771635 doi: 10.1089/ten.2006.12.1213 pmcid: 16771635
Crapo PM, Gilbert TW, Badylak SF (2011) An overview of tissue and whole organ decellularization processes. Biomaterials 32(12):3233–3243
pubmed: 21296410 pmcid: 3084613 doi: 10.1016/j.biomaterials.2011.01.057
Daly AB, Wallis JM, Borg ZD, Bonvillain RW, Deng B, Ballif BA et al (2012) Initial binding and recellularization of decellularized mouse lung scaffolds with bone marrow-derived mesenchymal stromal cells. Tissue Eng Part A 18(1–2):1–16
pubmed: 21756220 doi: 10.1089/ten.tea.2011.0301 pmcid: 21756220
De Santis MM, Bölükbas DA, Lindstedt S, Wagner DE (2018) How to build a lung: latest advances and emerging themes in lung bioengineering. Eur Respir J 52(1)
Doryab A, Heydarian M, Amoabediny G, Sadroddiny E, Mahfouzi S (2017) Recellularization on acellular lung tissue scaffold using perfusion-based bioreactor: an online monitoring strategy. J Med Biol Eng 37(1):53–62
doi: 10.1007/s40846-016-0205-1
Douglas WH, Moorman GW, Teel RW (1976) The formation of histotypic structures from monodisperse fetal rat lung cells cultured on a three-dimensional substrate. Vitro 12(5):373–381
doi: 10.1007/BF02796315
Fischer SN, Johnson JK, Baran CP, Newland CA, Marsh CB, Lannutti JJ (2011) Organ-derived coatings on electrospun nanofibers as ex vivo microenvironments. Biomaterials 32(2):538–546
pubmed: 20875916 doi: 10.1016/j.biomaterials.2010.08.104 pmcid: 20875916
Garcia O, Carraro G, Navarro S, Bertoncello I, McQualter J, Driscoll B et al (2012) Cell-based therapies for lung disease. Br Med Bull 101(1):147–161
pubmed: 22279079 pmcid: 3695661 doi: 10.1093/bmb/ldr051
Ghaedi M, Calle EA, Mendez JJ, Gard AL, Balestrini J, Booth A et al (2013) Human iPS cell–derived alveolar epithelium repopulates lung extracellular matrix. J Clin Investig 123(11):4950–4962
pubmed: 24135142 pmcid: 3809786 doi: 10.1172/JCI68793
Ghaedi M, Le AV, Hatachi G, Beloiartsev A, Rocco K, Sivarapatna A et al (2018) Bioengineered lungs generated from human iPSCs-derived epithelial cells on native extracellular matrix. J Tissue Eng Regen Med 12(3):e1623–e1635
pubmed: 29024475 doi: 10.1002/term.2589 pmcid: 29024475
Gilpin SE, Ren X, Okamoto T, Guyette JP, Mou H, Rajagopal J et al (2014) Enhanced lung epithelial specification of human induced pluripotent stem cells on decellularized lung matrix. Ann Thorac Surg 98(5):1721–1729
pubmed: 25149047 pmcid: 4252658 doi: 10.1016/j.athoracsur.2014.05.080
Gilpin SE, Charest JM, Ren X, Ott HC (2016) Bioengineering lungs for transplantation. Thorac Cardiovasc Surg 26(2):163–171
Goolaerts A, Pellan-Randrianarison N, Larghero J, Vanneaux V, Uzunhan Y, Gille T et al (2014) Conditioned media from mesenchymal stromal cells restore sodium transport and preserve epithelial permeability in an in vitro model of acute alveolar injury. Am J Physiol-Lung Cell Mol Physiol 306(11):L975–L985
pubmed: 24682451 pmcid: 4042188 doi: 10.1152/ajplung.00242.2013
Gotts JE, Matthay MA (2012) Mesenchymal stem cells and the stem cell niche: a new chapter. Am J Physiol-Lung Cell Mol Physiol 302(11):L1147–L1149
pubmed: 22505672 doi: 10.1152/ajplung.00122.2012 pmcid: 22505672
Hoganson DM, Pryor HI, Vacanti JP (2008) Tissue engineering and organ structure: a vascularized approach to liver and lung. Pediatr Res 63(5):520–526
pubmed: 18427297 doi: 10.1203/01.pdr.0000305879.38476.0c pmcid: 18427297
Hoganson DM, Pryor HI II, Bassett EK, Spool ID, Vacanti JP (2011) Lung assist device technology with physiologic blood flow developed on a tissue engineered scaffold platform. Lab Chip 11(4):700–707
pubmed: 21152606 doi: 10.1039/C0LC00158A pmcid: 21152606
Huang SX, Islam MN, O’neill J, Hu Z, Yang Y-G, Chen Y-W et al (2014) Efficient generation of lung and airway epithelial cells from human pluripotent stem cells. Nat Biotechnol. 32(1):84
Jiang Y, Jahagirdar BN, Reinhardt RL, Schwartz RE, Keene CD, Ortiz-Gonzalez XR et al (2002) Pluripotency of mesenchymal stem cells derived from adult marrow. Nature 418(6893):41–49
pubmed: 12077603 doi: 10.1038/nature00870 pmcid: 12077603
Kajbafzadeh A-M, Payabvash S, Salmasi AH, Monajemzadeh M, Tavangar SM (2006) Smooth muscle cell apoptosis and defective neural development in congenital ureteropelvic junction obstruction. J Urol 176(2):718–723
pubmed: 16813927 doi: 10.1016/j.juro.2006.03.041 pmcid: 16813927
Kajbafzadeh A, Sabetkish N, Sabetkish S, Tavangar S, Beigi RH, Talebi M et al (2015a) Lung tissue engineering and preservation of alveolar microstructure using a novel casting method. Biotech Histochem 90(2):111–123
pubmed: 25268847 doi: 10.3109/10520295.2014.957724
Kajbafzadeh AM, Khorramirouz R, Akbarzadeh A, Sabetkish S, Sabetkish N, Saadat P et al (2015b) A novel technique for simultaneous whole-body and multi-organ decellularization: umbilical artery catheterization as a perfusion-based method in a sheep foetus model. Int J Exp Pathol 96(2):116–132
pubmed: 26031202 pmcid: 4459805 doi: 10.1111/iep.12124
Khorramirouz R, Sabetkish S, Akbarzadeh A, Muhammadnejad A, Heidari R, Kajbafzadeh A-M (2014) Effect of three decellularisation protocols on the mechanical behaviour and structural properties of sheep aortic valve conduits. Adv Med Sci 59(2):299–307
pubmed: 25260036 doi: 10.1016/j.advms.2014.08.004 pmcid: 25260036
Kreuter M, Witt S, Polke M, Waelscher J, Schwarzkopf L (2018) Financial burden of interstitial lung disease-a claims data based study. C41. Health services research in pulmonary disease. American Thoracic Society. p A4927-A
Kuttan R, Spall R, Duhamel R, Sipes I, Meezan E, Brendel K (1981) Preparation and composition of alveolar extracellular matrix and incorporated basement membrane. Lung 159(1):333–345
pubmed: 7300440 doi: 10.1007/BF02713933 pmcid: 7300440
Macchiarini P, Jungebluth P, Go T, Asnaghi MA, Rees LE, Cogan TA et al (2008) Clinical transplantation of a tissue-engineered airway. Lancet 372(9655):2023–2030
pubmed: 19022496 doi: 10.1016/S0140-6736(08)61598-6 pmcid: 19022496
Majka SM, Beutz MA, Hagen M, Izzo AA, Voelkel N, Helm KM (2005) Identification of novel resident pulmonary stem cells: form and function of the lung side population. Stem Cells 23(8):1073–1081
pubmed: 15987674 doi: 10.1634/stemcells.2005-0039 pmcid: 15987674
Mandrycky C, Phong K, Zheng Y (2017) Tissue engineering toward organ-specific regeneration and disease modeling. MRS Commun 7(3):332–347
pubmed: 29750131 pmcid: 5939579 doi: 10.1557/mrc.2017.58
McAuley DF, Curley GF, Hamid UI, Laffey JG, Abbott J, McKenna DH et al (2014) Clinical grade allogeneic human mesenchymal stem cells restore alveolar fluid clearance in human lungs rejected for transplantation. Am J Physiol-Lung Cell Mol Physiol 306(9):L809–L815
pubmed: 24532289 pmcid: 4010648 doi: 10.1152/ajplung.00358.2013
Mendez JJ, Ghaedi M, Steinbacher D, Niklason LE (2014) Epithelial cell differentiation of human mesenchymal stromal cells in decellularized lung scaffolds. Tissue Eng Part A 20(11–12):1735–1746
pubmed: 24393055 pmcid: 4029048 doi: 10.1089/ten.tea.2013.0647
Mondrinos MJ, Koutzaki S, Jiwanmall E, Li M, Dechadarevian J-P, Lelkes PI et al (2006) Engineering three-dimensional pulmonary tissue constructs. Tissue Eng 12(4):717–728
pubmed: 16674286 doi: 10.1089/ten.2006.12.717 pmcid: 16674286
Neuman RE, Logan MA (1950) The determination of hydroxyproline. J Biol Chem 184(1):299–306
pubmed: 15421999 doi: 10.1016/S0021-9258(19)51149-8 pmcid: 15421999
Nichols JE, Niles J, Riddle M, Vargas G, Schilagard T, Ma L et al (2013) Production and assessment of decellularized pig and human lung scaffolds. Tissue Eng Part A 19(17–18):2045–2062
pubmed: 23638920 pmcid: 3725800 doi: 10.1089/ten.tea.2012.0250
O’Neill JD, Anfang R, Anandappa A, Costa J, Javidfar J, Wobma HM et al (2013) Decellularization of human and porcine lung tissues for pulmonary tissue engineering. Ann Thorac Surg 96(3):1046–1056
pubmed: 23870827 pmcid: 4033908 doi: 10.1016/j.athoracsur.2013.04.022
Orlando G, Farney AC, Iskandar SS, Mirmalek-Sani S-H, Sullivan DC, Moran E et al (2012) Production and implantation of renal extracellular matrix scaffolds from porcine kidneys as a platform for renal bioengineering investigations. Ann Surg 256(2):363–370
pubmed: 22691371 doi: 10.1097/SLA.0b013e31825a02ab pmcid: 22691371
Ott HC, Clippinger B, Conrad C, Schuetz C, Pomerantseva I, Ikonomou L et al (2010) Regeneration and orthotopic transplantation of a bioartificial lung. Nat Med 16(8):927
pubmed: 20628374 doi: 10.1038/nm.2193 pmcid: 20628374
Panoskaltsis-Mortari A (2015) Bioreactor development for lung tissue engineering. Current Transplant Rep 2(1):90–97
doi: 10.1007/s40472-014-0048-z
Petersen TH, Calle EA, Zhao L, Lee EJ, Gui L, Raredon MB et al (2010) Tissue-engineered lungs for in vivo implantation. Science 329(5991):538–541
pubmed: 20576850 pmcid: 3640463 doi: 10.1126/science.1189345
Raredon MSB, Ghaedi M, Calle EA, Niklason LE (2015) A rotating bioreactor for scalable culture and differentiation of respiratory epithelium. Cell Med 7(3):109–121
pubmed: 26858899 doi: 10.3727/215517914X681794 pmcid: 26858899
Ren X, Moser PT, Gilpin SE, Okamoto T, Wu T, Tapias LF et al (2015) Engineering pulmonary vasculature in decellularized rat and human lungs. Nat Biotechnol 33(10):1097
pubmed: 26368048 doi: 10.1038/nbt.3354 pmcid: 26368048
Rippon HJ, Polak JM, Qin M, Bishop AE (2006) Derivation of distal lung epithelial progenitors from murine embryonic stem cells using a novel three-step differentiation protocol. Stem Cells 24(5):1389–1398
pubmed: 16456134 doi: 10.1634/stemcells.2005-0465 pmcid: 16456134
Rosen C, Shezen E, Aronovich A, Klionsky YZ, Yaakov Y, Assayag M et al (2015) Preconditioning allows engraftment of mouse and human embryonic lung cells, enabling lung repair in mice. Nat Med 21(8):869
pubmed: 26168294 doi: 10.1038/nm.3889 pmcid: 26168294
Samadikuchaksaraei A, Cohen S, Isaac K, Rippon HJ, Polak JM, Bielby RC et al (2006) Derivation of distal airway epithelium from human embryonic stem cells. Tissue Eng 12(4):867–875
pubmed: 16674299 doi: 10.1089/ten.2006.12.867 pmcid: 16674299
Sato M, Okada Y, Oto T, Minami M, Shiraishi T, Nagayasu T et al (2014) Registry of the Japanese society of lung and heart-lung transplantation: official Japanese lung transplantation report, 2014. Gen Thorac Cardiovasc Surg 62(10):594–601
pubmed: 24842125 doi: 10.1007/s11748-014-0418-6 pmcid: 24842125
Scarritt ME, Pashos NC, Bunnell BA (2015) A review of cellularization strategies for tissue engineering of whole organs. Front Bioeng Biotechnol 3:43
pubmed: 25870857 pmcid: 4378188 doi: 10.3389/fbioe.2015.00043
Shannon JM, Hyatt BA (2004) Epithelial-mesenchymal interactions in the developing lung. Annu Rev Physiol 66:625–645
pubmed: 14977416 doi: 10.1146/annurev.physiol.66.032102.135749 pmcid: 14977416
Shojaie S, Ermini L, Ackerley C, Wang J, Chin S, Yeganeh B et al (2015) Acellular lung scaffolds direct differentiation of endoderm to functional airway epithelial cells: requirement of matrix-bound HS proteoglycans. Stem Cell Rep 4(3):419–430
doi: 10.1016/j.stemcr.2015.01.004
Sokocevic D, Bonenfant NR, Wagner DE, Borg ZD, Lathrop MJ, Lam YW et al (2013) The effect of age and emphysematous and fibrotic injury on the re-cellularization of de-cellularized lungs. Biomaterials 34(13):3256–3269
pubmed: 23384794 pmcid: 4215729 doi: 10.1016/j.biomaterials.2013.01.028
Sueblinvong V, Loi R, Eisenhauer PL, Bernstein IM, Suratt BT, Spees JL et al (2008) Derivation of lung epithelium from human cord blood–derived mesenchymal stem cells. Am J Respir Crit Care Med 177(7):701–711
pubmed: 18063840 doi: 10.1164/rccm.200706-859OC pmcid: 18063840
Sullivan DC, Mirmalek-Sani S-H, Deegan DB, Baptista PM, Aboushwareb T, Atala A et al (2012) Decellularization methods of porcine kidneys for whole organ engineering using a high-throughput system. Biomaterials 33(31):7756–7764
pubmed: 22841923 doi: 10.1016/j.biomaterials.2012.07.023 pmcid: 22841923
Takahashi K, Chen F, Ikeda T, Doi H, Nakamura H, Yanagi S et al (2013) Single-lobe lung transplantation for rapidly deteriorating pulmonary venoocclusive disease. Ann Thorac Surg 95(2):689–691
pubmed: 23336879 doi: 10.1016/j.athoracsur.2012.06.031 pmcid: 23336879
Tebyanian H, Karami A, Nourani MR, Motavallian E, Barkhordari A, Yazdanian M et al (2019) Lung tissue engineering: an update. J Cell Physiol 234(11):19256–19270
pubmed: 30972749 doi: 10.1002/jcp.28558 pmcid: 30972749
Tudorache I, Cebotari S, Sturz G, Kirsch L, Hurschler C, Hilfiker A et al. (2007) Tissue engineering of heart valves: biomechanical and morphological properties of decellularized heart valves. J Heart Valve Dis 16(5):567–73. discussion 74
Vranken BV, Romanska H, Polak J, Rippon H, Shannon J, Bishop A (2005) Coculture of embryonic stem cells with pulmonary mesenchyme: a microenvironment that promotes differentiation of pulmonary epithelium. Tissue Eng 11(7–8):1177–1187
pubmed: 16144454 doi: 10.1089/ten.2005.11.1177 pmcid: 16144454
Wagner DE, Bonvillain RW, Jensen T, Girard ED, Bunnell BA, Finck CM et al (2013) Can stem cells be used to generate new lungs? Ex vivo lung bioengineering with decellularized whole lung scaffolds. Respirology 18(6):895–911
pubmed: 23614471 doi: 10.1111/resp.12102 pmcid: 23614471
Wagner DE, Bonenfant NR, Parsons CS, Sokocevic D, Brooks EM, Borg ZD et al (2014a) Comparative decellularization and recellularization of normal versus emphysematous human lungs. Biomaterials 35(10):3281–3297
pubmed: 24461327 pmcid: 4215725 doi: 10.1016/j.biomaterials.2013.12.103
Wagner DE, Bonenfant NR, Sokocevic D, DeSarno MJ, Borg ZD, Parsons CS et al (2014b) Three-dimensional scaffolds of acellular human and porcine lungs for high throughput studies of lung disease and regeneration. Biomaterials 35(9):2664–2679
pubmed: 24411675 pmcid: 4215726 doi: 10.1016/j.biomaterials.2013.11.078
Wallis JM, Borg ZD, Daly AB, Deng B, Ballif BA, Allen GB et al (2012) Comparative assessment of detergent-based protocols for mouse lung de-cellularization and re-cellularization. Tissue Eng Part C Methods 18(6):420–432
pubmed: 22165818 pmcid: 3358122 doi: 10.1089/ten.tec.2011.0567
Weibel ER (2017) Lung morphometry: the link between structure and function. Cell Tissue Res 367(3):413–426
pubmed: 27981379 doi: 10.1007/s00441-016-2541-4 pmcid: 27981379
Wong AP, Keating A, Lu W-Y, Duchesneau P, Wang X, Sacher A et al (2009) Identification of a bone marrow–derived epithelial-like population capable of repopulating injured mouse airway epithelium. J Clin Investig 119(2):336–348
pubmed: 19164856 pmcid: 2631300
Wong AP, Bear CE, Chin S, Pasceri P, Thompson TO, Huan L-J et al (2012) Directed differentiation of human pluripotent stem cells into mature airway epithelia expressing functional CFTR protein. Nat Biotechnol 30(9):876
pubmed: 22922672 pmcid: 3994104 doi: 10.1038/nbt.2328
Zhou H, Kitano K, Ren X, Rajab TK, Wu M, Gilpin SE et al (2018) Bioengineering human lung grafts on porcine matrix. Ann Surg 267(3):590–598
pubmed: 28085694 doi: 10.1097/SLA.0000000000002129 pmcid: 28085694

Auteurs

Shabnam Sabetkish (S)

Pediatric Urology and Regenerative Medicine Research Center, Section of Tissue Engineering and Stem Cells Therapy, Children's Medical Center, Pediatric Center of Excellence, Tehran University of Medical Sciences, Tehran, Iran, No. 62, Dr. Gharib's Street, Keshavarz Boulevard, 1419433151, Tehran, Iran.

Abdol-Mohammad Kajbafzadeh (AM)

Pediatric Urology and Regenerative Medicine Research Center, Section of Tissue Engineering and Stem Cells Therapy, Children's Medical Center, Pediatric Center of Excellence, Tehran University of Medical Sciences, Tehran, Iran, No. 62, Dr. Gharib's Street, Keshavarz Boulevard, 1419433151, Tehran, Iran. kajbafzd@sina.tums.ac.ir.

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