LGR5, HES1 and ATOH1 in Young Rectal Cancer Patients in Egyptian.
Gene expression
Young colon cancer
cance stem cells chemotherapy prognosis
Journal
Asian Pacific journal of cancer prevention : APJCP
ISSN: 2476-762X
Titre abrégé: Asian Pac J Cancer Prev
Pays: Thailand
ID NLM: 101130625
Informations de publication
Date de publication:
01 Sep 2021
01 Sep 2021
Historique:
received:
08
05
2021
entrez:
28
9
2021
pubmed:
29
9
2021
medline:
24
12
2021
Statut:
epublish
Résumé
The study aimed to delineate the gene expression profile of LGR5, HES1 and ATOH1 in young Egyptian rectal cancer (RC) patients and investigate the correlation between expression profiles and clinical outcome. The study was conducted on 30 young Egyptian RC patients. Expression study of LGR5, HES1 and ATOH1 were performed by quantitative PCR (QPCR) based on comparative Cq method after normalization to adjacent non tumor tissues and ACTB as a reference gene. Patients were followed up for assessment of response to neoadjuvant chemoradiotherapy (CRT) based on revised RECIST1.1. The study detected overexpression of LGR5 and HES1 and down-regulation of ATOH1 in human RC tissues compared to non- tumor tissues. High expression of LGR5 was correlated with more depth of tumor invasion, lymph node (LN) metastasis, advanced cTNM stage and mesorectal fascia (MRF) involvement. More prominently, high LGR5 expression level was associated with poor response to CRT. LGR5 was suggested as unfavorable prognostic biomarker for RC. Conversely, HES1 and ATOH1 expression did not show significant association with most of the studied clinical criteria nor response to CRT. Still, HES1 and ATOH1 were significantly and inversely associated with presence of mucinous component. High LGR5 expression is indicative of poor prognosis among young Egyptian RC patients and is proposed as a predictive marker of resistance to neoadjuvant CRT. However, HES1 and ATOH1 expressions were not prognostic nor predictive of response to CRT. Overall, LGR5, HES1 and ATOH1 gene expression patterns among young onset RC patients, are in line with patterns encountered in older age groups.
Identifiants
pubmed: 34582650
doi: 10.31557/APJCP.2021.22.9.2819
pmc: PMC8850894
pii:
doi:
Substances chimiques
ATOH1 protein, human
0
Basic Helix-Loop-Helix Transcription Factors
0
LGR5 protein, human
0
Receptors, G-Protein-Coupled
0
Transcription Factor HES-1
0
HES1 protein, human
149348-15-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
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