Bifidobacteria Strain Typing by Fourier Transform Infrared Spectroscopy.
Bifidobacterium
FTIR spectroscopy
MLST
PFGE
live biotherapeutic products
probiotics
strain typing
Journal
Frontiers in microbiology
ISSN: 1664-302X
Titre abrégé: Front Microbiol
Pays: Switzerland
ID NLM: 101548977
Informations de publication
Date de publication:
2021
2021
Historique:
received:
09
04
2021
accepted:
09
08
2021
entrez:
30
9
2021
pubmed:
1
10
2021
medline:
1
10
2021
Statut:
epublish
Résumé
Fourier transform infrared (FTIR) spectroscopy, a technology traditionally used in chemistry to determine the molecular composition of a wide range of sample types, has gained growing interest in microbial typing. It is based on the different vibrational modes of the covalent bonds between atoms of a given sample, as bacterial cells, induced by the absorption of infrared radiation. This technique has been largely used for the study of pathogenic species, especially in the clinical field, and has been proposed also for the typing at different subspecies levels. The high throughput, speed, low cost, and simplicity make FTIR spectroscopy an attractive technique also for industrial applications, in particular, for probiotics. The aim of this study was to compare FTIR spectroscopy with established genotyping methods, pulsed-field gel electrophoresis (PFGE), whole-genome sequencing (WGS), and multilocus sequence typing (MLST), in order to highlight the FTIR spectroscopy potential discriminatory power at strain level. Our study focused on bifidobacteria, an important group of intestinal commensals generally recognized as probiotics. For their properties in promoting and maintaining health, bifidobacteria are largely marketed by the pharmaceutical, food, and dairy industries. Strains belonging to
Identifiants
pubmed: 34589064
doi: 10.3389/fmicb.2021.692975
pmc: PMC8473902
doi:
Types de publication
Journal Article
Langues
eng
Pagination
692975Informations de copyright
Copyright © 2021 Deidda, Bozzi Cionci, Cordovana, Campedelli, Fracchetti, Di Gioia, Ambretti and Pane.
Déclaration de conflit d'intérêts
MP and FD are employees of Probiotical Research S.r.L., Novara, Italy. MC is employee of Bruker Daltonik, Bremen, Germany. FF and IC are employees of Microbion S.r.L., San Giovanni Lupatoto, Verona, Italy. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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