Preclinical study of peripheral nerve regeneration using nerve guidance conduits based on polyhydroxyalkanaotes.
nerve regeneration
nerve tissue engineering
peripheral nerve regeneration
Journal
Bioengineering & translational medicine
ISSN: 2380-6761
Titre abrégé: Bioeng Transl Med
Pays: United States
ID NLM: 101689146
Informations de publication
Date de publication:
Sep 2021
Sep 2021
Historique:
received:
02
11
2020
revised:
09
03
2021
accepted:
14
03
2021
entrez:
30
9
2021
pubmed:
1
10
2021
medline:
1
10
2021
Statut:
epublish
Résumé
Nerve guidance conduits (NGCs) are used as an alternative to the "gold standard" nerve autografting, preventing the need for surgical intervention required to harvest autologous nerves. However, the regeneration outcomes achieved with the current NGCs are only comparable with autografting when the gap is short (less than 10 mm). In the present study, we have developed NGCs made from a blend of polyhydroxyalkanoates, a family of natural resorbable polymers. Hollow NGCs made from a 75:25 poly(3-hydroxyoctanoate)/poly(3-hydroxybutyrate) blend (PHA-NGCs) were manufactured using dip-molding. These PHA-NGCs showed appropriate flexibility for peripheral nerve regeneration. In vitro cell studies performed using RT4-D6P2T rat Schwann cell line confirmed that the material is capable of sustaining cell proliferation and adhesion. PHA-NGCs were then implanted in vivo to repair 10 mm gaps of the median nerve of female Wistar rats for 12 weeks. Functional evaluation of the regenerated nerve using the grasping test showed that PHA-NGCs displayed similar motor recovery as the autograft, starting from week 7. Additionally, nerve cross-sectional area, density and number of myelinated cells, as well as axon diameter, fiber diameter, myelin thickness and g-ratio obtained using the PHA-NGCs were found comparable to an autograft. This preclinical data confirmed that the PHA-NGCs are indeed highly promising candidates for peripheral nerve regeneration.
Identifiants
pubmed: 34589600
doi: 10.1002/btm2.10223
pii: BTM210223
pmc: PMC8459605
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e10223Informations de copyright
© 2021 The Authors. Bioengineering & Translational Medicine published by Wiley Periodicals LLC on behalf of The American Institute of Chemical Engineers.
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
Références
Cell. 2015 Aug 27;162(5):1127-39
pubmed: 26279190
Scand J Trauma Resusc Emerg Med. 2018 May 15;26(1):40
pubmed: 29764455
Acta Neurochir (Wien). 2017 Feb;159(2):281-290
pubmed: 27999953
Curr Opin Neurobiol. 2016 Aug;39:38-46
pubmed: 27128880
Br J Plast Surg. 2002 Apr;55(3):235-40
pubmed: 12041978
Biomaterials. 2010 Mar;31(7):1691-8
pubmed: 19962755
Trends Endocrinol Metab. 2014 Jan;25(1):42-52
pubmed: 24140022
J Control Release. 2010 Apr 19;143(2):168-74
pubmed: 20035811
J Vet Intern Med. 2014 Mar-Apr;28(2):646-55
pubmed: 24417498
ASAIO J. 2013 Nov-Dec;59(6):651-9
pubmed: 24172271
J R Soc Interface. 2012 Feb 7;9(67):202-21
pubmed: 22090283
Neurol Res. 2004 Mar;26(2):151-60
pubmed: 15072634
Neurosurg Focus. 2009 Feb;26(2):E5
pubmed: 19435445
Front Cell Neurosci. 2019 Jun 28;13:288
pubmed: 31316355
J Vis Exp. 2018 Jul 3;(137):
pubmed: 30035773
PLoS One. 2009 Nov 13;4(11):e7754
pubmed: 19915661
Hand Clin. 2017 Aug;33(3):545-550
pubmed: 28673630
Int J Mol Sci. 2016 Dec 14;17(12):
pubmed: 27983642
J Surg Res. 2003 Oct;114(2):133-9
pubmed: 14559438
Arch Neurol Psychiatry. 1948 Mar;59(3):322-36
pubmed: 18874264
J Biomater Sci Polym Ed. 2004;15(12):1521-31
pubmed: 15696797
J Neurosci Methods. 2003 Jul 15;127(1):43-7
pubmed: 12865147
Exp Neurol. 2010 May;223(1):86-101
pubmed: 19769967
Behav Brain Res. 1990 Dec 7;41(1):49-59
pubmed: 2073355
J Neurosci Methods. 2009 Apr 30;179(1):51-7
pubmed: 19428511
Eur J Neurosci. 2016 Feb;43(3):271-86
pubmed: 26228942
J Tissue Eng Regen Med. 2019 Sep;13(9):1581-1594
pubmed: 31185133
Ann Anat. 2000 Jan;182(1):23-34
pubmed: 10668555
Biomaterials. 2001 May;22(10):1077-84
pubmed: 11352088
J Comp Neurol. 2000 Nov 20;427(3):333-9
pubmed: 11054696
Biomaterials. 2009 Jan;30(2):217-25
pubmed: 18849069
Exp Neurol. 2017 Jan;287(Pt 3):348-357
pubmed: 27094121
Biomaterials. 2010 May;31(14):3967-75
pubmed: 20153524
Injury. 2012 May;43(5):553-72
pubmed: 21269624
Brain Res. 1983 Jan 17;259(1):41-56
pubmed: 6824935
Biomaterials. 2012 Jan;33(1):59-71
pubmed: 22005069
PLoS One. 2013;8(2):e57157
pubmed: 23468923
Scand J Trauma Resusc Emerg Med. 2018 Sep 10;26(1):76
pubmed: 30201025
Scand J Plast Reconstr Surg Hand Surg. 2005;39(3):129-37
pubmed: 16019744
J Biomed Mater Res A. 2017 May;105(5):1383-1392
pubmed: 28152586