T lymphocyte and monocyte subsets are dysregulated in type 1 diabetes patients with peripheral neuropathic pain.

CD27 Chronic pain Diabetic neuropathy FlowSOM Immunophenotyping MAPKAPK2 MK2 Mass cytometry SPADE Type 1 diabetes

Journal

Brain, behavior, & immunity - health
ISSN: 2666-3546
Titre abrégé: Brain Behav Immun Health
Pays: United States
ID NLM: 101759062

Informations de publication

Date de publication:
Aug 2021
Historique:
received: 05 04 2021
revised: 31 05 2021
accepted: 04 06 2021
entrez: 30 9 2021
pubmed: 1 10 2021
medline: 1 10 2021
Statut: epublish

Résumé

Diabetic neuropathic pain is a common and devastating complication of type 1 diabetes, but the mechanism by which it develops and persists is yet to be fully elucidated. This study utilised high-dimensional suspension mass cytometry in a pilot cohort to investigate differences in peripheral blood immunophenotypes between type 1 diabetes patients with (n ​= ​9) and without (n ​= ​9) peripheral neuropathic pain. The abundance and activation of several leukocyte subsets were investigated with unsupervised clustering approaches FlowSOM and SPADE, as well as by manual gating. Major findings included a proportional increase in CD4

Identifiants

pubmed: 34589782
doi: 10.1016/j.bbih.2021.100283
pii: S2666-3546(21)00086-7
pmc: PMC8474166
doi:

Types de publication

Journal Article

Langues

eng

Pagination

100283

Informations de copyright

© 2021 The Authors.

Déclaration de conflit d'intérêts

The authors declare no conflicts of interest.

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Auteurs

Jayden A O'Brien (JA)

School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Brain and Mind Centre, 94 Mallett St, Camperdown, NSW, 2050, Australia.

Helen M McGuire (HM)

Discipline of Pathology, Faculty of Medicine and Health, The University of Sydney, NSW, Australia.
Ramaciotti Facility for Human Systems Biology, Charles Perkins Centre, The University of Sydney, NSW, Australia.

Diana Shinko (D)

Ramaciotti Facility for Human Systems Biology, Charles Perkins Centre, The University of Sydney, NSW, Australia.
Sydney Cytometry, The University of Sydney, NSW, Australia.

Barbara Fazekas de St Groth (B)

Discipline of Pathology, Faculty of Medicine and Health, The University of Sydney, NSW, Australia.
Ramaciotti Facility for Human Systems Biology, Charles Perkins Centre, The University of Sydney, NSW, Australia.

Marc A Russo (MA)

Genesis Research Services, Broadmeadow, NSW, Australia.

Dominic Bailey (D)

Genesis Research Services, Broadmeadow, NSW, Australia.
Hunter Medical Research Institute, New Lambton Heights, NSW, Australia.

Danielle M Santarelli (DM)

Genesis Research Services, Broadmeadow, NSW, Australia.

Katie Wynne (K)

Department of Diabetes and Endocrinology, John Hunter Hospital, Newcastle, NSW, Australia.
School of Medicine and Public Health, University of Newcastle, NSW, Australia.

Paul J Austin (PJ)

School of Medical Sciences, Faculty of Medicine and Health, The University of Sydney, Brain and Mind Centre, 94 Mallett St, Camperdown, NSW, 2050, Australia.

Classifications MeSH