A laser-induced graphene electrochemical immunosensor for label-free CEA monitoring in serum.

The cost-effective construction of self-designed conductive graphene patterns is crucial to the fabrication of graphene-based electrochemical devices. Here, a label-free carcinoembryonic antigen (CEA) electrochemical immunosensor is developed based on the surface engineering of a laser-induced graphene (LIG)/Au electrode. The LIG electrode was produced with a smart and inexpensive 450 nm semiconductor laser through three electrode patterns under ambient conditions. Then the LIG/Au electrode was organized by conformal anchoring of Au nanoparticles (NPs) on the LIG work area using chloroauric acid as the precursor. Good electrochemical activity with improved conductivity of the LIG/Au electrode was obtained under optimized conditions of laser intensity, carving depth, and chlorogenic acid dosage, to name a few. The LIG/Au electrode was carbonylated based on Au-S∼COOH using 11-mercaptoundecanoic acid (MUA). The antibody was covalently bound on the work area to form a label-free immunosensor. The constructed immunosensor shows high sensitivity with a good response in the range of low concentrations from 0.01 to 100 ng mL