Recording Temporal Signals with Minutes Resolution Using Enzymatic DNA Synthesis.
Journal
Journal of the American Chemical Society
ISSN: 1520-5126
Titre abrégé: J Am Chem Soc
Pays: United States
ID NLM: 7503056
Informations de publication
Date de publication:
13 10 2021
13 10 2021
Historique:
pubmed:
1
10
2021
medline:
19
2
2022
entrez:
30
9
2021
Statut:
ppublish
Résumé
Employing DNA as a high-density data storage medium has paved the way for next-generation digital storage and biosensing technologies. However, the multipart architecture of current DNA-based recording techniques renders them inherently slow and incapable of recording fluctuating signals with subhour frequencies. To address this limitation, we developed a simplified system employing a single enzyme, terminal deoxynucleotidyl transferase (TdT), to transduce environmental signals into DNA. TdT adds nucleotides to the 3'-ends of single-stranded DNA (ssDNA) in a template-independent manner, selecting bases according to inherent preferences and environmental conditions. By characterizing TdT nucleotide selectivity under different conditions, we show that TdT can encode various physiologically relevant signals such as Co
Identifiants
pubmed: 34591459
doi: 10.1021/jacs.1c07331
pmc: PMC8982284
mid: NIHMS1765996
doi:
Substances chimiques
DNA Nucleotidylexotransferase
EC 2.7.7.31
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
16630-16640Subventions
Organisme : NIH HHS
ID : S10 OD026963
Pays : United States
Organisme : NIMH NIH HHS
ID : R01 MH103910
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA060553
Pays : United States
Organisme : NINDS NIH HHS
ID : UF1 NS107697
Pays : United States
Organisme : NIGMS NIH HHS
ID : T32 GM008449
Pays : United States
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