Method Development for a Short-Term 7-Day Toxicity Test with Unionid Mussels.
Interlaboratory variability
Sodium chloride
Species sensitivity
Test methods
Whole effluent toxicity
Journal
Environmental toxicology and chemistry
ISSN: 1552-8618
Titre abrégé: Environ Toxicol Chem
Pays: United States
ID NLM: 8308958
Informations de publication
Date de publication:
12 2021
12 2021
Historique:
revised:
14
06
2021
received:
05
05
2021
accepted:
28
09
2021
pubmed:
1
10
2021
medline:
24
3
2022
entrez:
30
9
2021
Statut:
ppublish
Résumé
The US Environmental Protection Agency's short-term freshwater effluent test methods include a fish (Pimephales promelas), a cladoceran (Ceriodaphnia dubia), and a green alga (Raphidocelis subcapitata). There is a recognized need for additional taxa to accompany the three standard species for effluent testing. An appropriate additional taxon is unionid mussels because mussels are widely distributed, live burrowed in sediment and filter particles from the water column for food, and exhibit high sensitivity to a variety of contaminants. Multiple studies were conducted to develop a relevant and robust short-term test method for mussels. We first evaluated the comparative sensitivity of two mussel species (Villosa constricta and Lampsilis siliquoidea) and two standard species (P. promelas and C. dubia) using two mock effluents prepared by mixing ammonia and five metals (cadmium, copper, nickel, lead, and zinc) or a field-collected effluent in 7-day exposures. Both mussel species were equally or more sensitive (more than two-fold) to effluents compared with the standard species. Next, we refined the mussel test method by first determining the best feeding rate of a commercial algal mixture for three age groups (1, 2, and 3 weeks old) of L. siliquoidea in a 7-day feeding experiment, and then used the derived optimal feeding rates to assess the sensitivity of the three ages of juveniles in a 7-day reference toxicant (sodium chloride [NaCl]) test. Juvenile mussels grew substantially (30%-52% length increase) when the 1- or 2-week-old mussels were fed 2 ml twice daily and the 3-week-old mussels were fed 3 ml twice daily. The 25% inhibition concentrations (IC25s) for NaCl were similar (314-520 mg Cl/L) among the three age groups, indicating that an age range of 1- to 3-week-old mussels can be used for a 7-day test. Finally, using the refined test method, we conducted an interlaboratory study among 13 laboratories to evaluate the performance of a 7-day NaCl test with L. siliquoidea. Eleven laboratories successfully completed the test, with more than 80% control survival and reliable growth data. The IC25s ranged from 296 to 1076 mg Cl/L, with a low (34%) coefficient of variation, indicating that the proposed method for L. siliquoidea has acceptable precision. Environ Toxicol Chem 2021;40:3392-3409. © 2021 SETAC.
Substances chimiques
Water Pollutants, Chemical
0
Types de publication
Journal Article
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
3392-3409Informations de copyright
© 2021 SETAC.
Références
ASTM International. (2020a). Standard guide for conducting laboratory toxicity tests with freshwater mussels (E2455-06 (2013)). In Annual book of ASTM standards. Volume 11.06.
ASTM International. (2020b). Standard test method for measuring the toxicity of sediment-associated contaminants with freshwater invertebrates (E1706-05 (2020). In Annual book of ASTM standards. Volume 11.06.
Augspurger, T. P., Wang, N., Kunz, J. L., & Ingersoll, C. G. (2014). Pollutant sensitivity of the endangered Tar River Spinymussel as assessed by single chemical and effluent toxicity tests: U.S. Fish and Wildlife Service, Ecological Services, Raleigh, NC. https://www.fws.gov/raleigh/pdfs/FinalP%5FSensi%5FTRS.pdf
Barnhart, M. C. (2006). Bucket of muckets: A compact recirculating system for rearing juvenile freshwater mussels. Aquaculture, 254, 227-233.
Besser, J. M., Brumbaugh, W. G., Kemble, N. E., Ivey, C. D., Kunz, J. L., Ingersoll, C. G., & Rudel, D. (2011). Toxicity of nickel-spiked freshwater sediments to benthic invertebrates-Spiking methodology, species sensitivity, and nickel bioavailability (U.S. Geological Survey Scientific Investigations Report 2011-5225). U.S. Geological Survey.
Besser, J. M., Ingersoll, C. G., Brumbaugh, W. G., Kemble, N. E., May, T. W., Wang, N., MacDonald, D. D., & Roberts, A. D. (2015). Toxicity of sediments from lead-zinc mining areas to juvenile freshwater mussels (Lampsilis siliquoidea) compared to standard test organisms. Environmental Toxicology and Chemistry, 34, 626-639.
Bringolf, R. B., Cope, W. G., Barnhart, M. C., Mosher, S., Lazaro, P. R., & Shea, D. (2007). Acute and chronic toxicity of pesticide formulations (atrazine, chlorpyrifos and permethrin) to glochidia and juveniles of Lampsilis siliquoidea. Environmental Toxicology and Chemistry, 26, 2086-2093.
Cope, W. G., Bringolf, R. B., Buchwalter, D. B., Newton, T. J., Ingersoll, C. G., Wang, N., Augspurger, T., Dwyer, F. J., Barnhart, M. C., Neves, R. J., & Hammer, E. (2008). Differential exposure, duration, and sensitivity of unionoidea bivalve life stages to environmental contaminants. Journal of the North American Benthological Society, 27(2).
Cummings, K. S., & Graf, D. L. (2010). Mollusca: Bivalvia. In J. H. Thorp & A. P. Covich (Eds.), Ecology and classification of North American freshwater invertebrates (3rd ed., pp. 309-384). Academic Press.
Eaton, A. D., Clesceri, L. S., Rice, E. W., & Greenberg, A. E. (2005). Standard methods for the examination of water and wastewater, 21st ed. American Public Health Association, Water Environment Federation, American Water Works Association.
Erickson, R. J. (2015). Toxicity Relationship Analysis Program (TRAP). Ver 1.30a. EPA/600/C-11/002. U.S. Environmental Protection Agency.
Gillis, P. L. (2011). Assessing the toxicity of sodium chloride to the glochidia of freshwater mussels: Implications for salinization of surface waters. Environmental Pollution, 159, 1702-1708.
Gillis, P. L., McGeer, J. C., Wilkie, W. P., Mackie, G. L., & Ackerman, J. D. (2010). The effect of natural dissolved organic carbon on the sensitivity of larval freshwater mussels (glochidia) to acute copper exposure. Environmental Toxicology and Chemistry, 29, 2519-2528.
Gillis, P. L., Mitchell, R. J., Schwalb, A. N., McNichols, K. A., Mackie, G. L., Wood, C. M., & Ackerman, J. D. (2008). Sensitivity of the glochidia of freshwater mussels to copper: Assessing the effect of water hardness and dissolved organic carbon on the sensitivity of endangered species. Aquatic Toxicology, 88, 137-145.
Gillis, P. L., Salerno, J., McKay, V., Bennett, C. J., Lemon, K. L. K., Rochfort, Q. J., & Prosser, R. S. (2021). Salt-laden winter runoff and freshwater mussels; Assessing the effect on early life stages in the laboratory and wild mussel populations in receiving waters. Archives of Environmental Contamination and Toxicology. Advance online publication. https://doi.org/10.1007/s00244-020-00791-2
Haag, W. R. (2012). North American freshwater mussels: Natural history, ecology, and conservation. Cambridge University Press.
Hach. (2020). User manual. Retrieved May 6, 2020, from: https://www.hach.com/asset-get.download.jsa?id=7648008627
Hanna. (2017). Iris visible spectrophotometer H1801. Retrieved May 6, 2020, from: https://www.hannainst.com/hubfs/product-manuals/MAN801%5F10%5F17.pdf%3FhsLang=en
Kolts, J. M., Brooks, M. L., Cantrell, B. D., Boese, C. J., Bell, B. R., & Meyer, J. S. (2008). Dissolved fraction of standard laboratory cladoceran food alters toxicity of waterborne silver to Ceriodaphnia dubia. Environmental Toxicology and Chemistry, 27, 1426-1434.
Kunz, J. L., Brunson, E. L., Barnhart, M. C., Glidewell, E. A., Wang, N., & Ingersoll, C. G. (2020). Pulsed flow-through auto-feeding beaker systems for the laboratory culture of juvenile freshwater mussels. Aquaculture, 520, 734959.
Kunz, J. L., Wang, N., Martinez, D., Dunn, S., Cleveland, D., & Steevens, J. A. (2021). The sensitivity of a unionid mussel (Lampsilis siliquoidea) to a permitted effluent and elevated potassium in the effluent. Environmental Toxicology and Chemistry, (this issue). https://doi.org/10.1002/etc.5221
Lopes-Lima, M., Sousa, R., Geist, J., Aldridge, D. C., Araujo, R., Bergengren, J., Bespalaya, Y., Bodis, E., Burlakova, L., Van Damme, D., Douda, K., Froufe, E., Georgiev, D., Gumpinger, C., Karatayev, A., Kebapci, U., Killeen, I., Lajtner, J., Larsen, B. M., … Zogaris, S. (2017). Conservation status of freshwater mussels in Europe: State of the art and future challenges. Biological Reviews, 92, 572-607.
Lydeard, C., Cowie, R. H., Ponder, W. F., Bogan, A. E., Bouchet, P., Clark, S. A., Cummings, K. S., Frest, T. J., Gargominy, O., Herbert, D. G., Hershler, R., Perez, K. E., Roth, B., Seddon, M., Strong, E. E., & Thompson, F. G. (2004). The global decline of nonmarine mollusks. BioScience, 54, 321-330.
Miao, J., Barnhart, M. C., Brunson, E. L., Hardesty, D. K., Ingersoll, C. G., & Wang, N. (2010). An evaluation of the influence of substrate on the response of juvenile freshwater mussels (Fatmucket, Lampsilis siliquoidea) in acute water exposure to ammonia. Environmental Toxicology and Chemistry, 29, 2112-2116.
NatureServe. (2020). NatureServe Explorer. Retrieved May 15, 2020, from: http://explorer.natureserve.org/servlet/NatureServe?searchName=Lampsilis+siliquoidea
Newton, T. J., & Bartsch, M. R. (2007). Lethal and sublethal effects of ammonia to juvenile Lampsilis mussels (Unionidae) in sediment and water-only exposures. Environmental Toxicology and Chemistry, 26, 2057-2065.
Raimondo, S., Lilavois, C., Lee, L., Augspurger, T., Wang, N., Ingersoll, C., Bauer, C., Hammer, E., & Barron, M. (2016). Assessing variability in chemical acute toxicity of unionid mussels: Influence of intra- and inter-laboratory testing, life stage, and species. Environmental Toxicology and Chemistry, 35, 750-758.
Salerno, J., Gillis, P., Khan, H., Burton, E., Deeth, L., Bennett, C., Sibley, P., & Prosser, R. (2020). Sensitivity of larval and juvenile freshwater mussels (unionidae) to ammonia, chloride, copper, potassium, and selected binary chemical mixtures. Environmental Pollution, 256, 113398.
Strayer, D. L., Downing, J. A., Haag, W. R., King, T. L., Layzer, J. B., Newton, T. J., & Nichols, S. J. (2004). Changing perspectives on pearly mussels, North America's most imperiled animals. BioScience, 54, 429-439.
U.S. Environmental Protection Agency. (1996). 1995 updates: Water quality criteria documents for the protection of aquatic life in ambient water. EPA/820-B-96-001. Office of Water.
U.S. Environmental Protection Agency. (1999). 1999 update of ambient water quality criteria for ammonia. EPA/822-R-99-014. Office of Water.
U.S. Environmental Protection Agency. (2001). Final report: Interlaboratory variability study of EPA short-term chronic and acute whole effluent toxicity test methods. Vol. 1. Vol. 2. EPA 821-B-01-004 and EPA 821-B-01-005. Office of Water, Office of Science and Technology.
U.S. Environmental Protection Agency. (2002). Short-term methods for estimating the chronic toxicity of effluents and receiving waters to freshwater organisms (4th ed.). EPA-821-R-02-013. Office of Water.
U.S. Environmental Protection Agency. (2007). Aquatic life ambient freshwater quality criteria-Copper, 2007 revision. EPA-822-R-07-001. Office of Water.
U.S. Environmental Protection Agency. (2012). National recommended water quality criteria tables. Retrieved May 17, 2012, from: https://www.epa.gov/wqc/national-recommended-water-quality-criteria-aquatic-life-criteria-table
U.S. Environmental Protection Agency. (2013). Aquatic life ambient water quality criteria for ammonia-Freshwater. EPA/822-R-13-001. Office of Water.
U.S. Environmental Protection Agency. (2014). Method 6020B: Inductively coupled plasma-mass spectrometry. Office of Water.
U.S. Environmental Protection Agency. (2020). Whole Effluent Toxicity (WET) NPDES Spreadsheet, Ver 2.0. https://www.epa.gov/npdes/whole-effluent-toxicity-wet-npdes-spreadsheet
U. S. National Institutes of Health. (2018). Image J. Retrieved May 15, 2018, from: https://imagej.nih.gov/nih-image/
Wang, N., Augspurger, T., Barnhart, M. C., Bidwell, J. R., Cope, W. G., Dwyer, F. J., Geis, S., Greer, I. E., Ingersoll, C. G., Kane, C. M., May, T. W., Neves, R. J., Newton, T. J., Roberts, A. D., & Whites, D. W. (2007c). Intra- and inter-laboratory variability in acute toxicity tests with glochidia and juveniles of freshwater mussels (Unionidae). Environmental Toxicology and Chemistry, 26, 2029-2035.
Wang, N., Consbrock, R., Ingersoll, C. G., & Barnhart, M. C. (2011a). Evaluation of influence of sediment on the sensitivity of a unionid mussel (Lampsilis siliquoidea) to ammonia in 28-day water exposures. Environmental Toxicology and Chemistry, 30, 2270-2276.
Wang, N., Dorman, R. A., Ingersoll, C. G., Hardest, D. K., Brumbaugh, W. G., Hammer, E. J., Bauer, C. R., & Mount, D. R. (2016). Acute and chronic toxicity of sodium sulfate to four freshwater organisms in water-only exposures. Environmental Toxicology and Chemistry, 35, 115-127.
Wang, N., Dorman, R. A., Ivey, C. D., Soucek, D. J., Dickinson, A., Kunz, B. K., Steevens, J. A., Hammer, E. J., & Bauer, C. R. (2020a). Acute and chronic toxicity of sodium nitrate and sodium sulfate to several freshwater organisms in water-only exposures. Environmental Toxicology and Chemistry, 39, 1071-1085.
Wang, N., Ingersoll, C. G., Greer, I. E., Hardesty, D. K., Ivey, C. D., Kunz, J. L., Brumbaugh, W. G., Dwyer, F. J., Roberts, A. D., Augspurger, T., Kane, C. M., Neves, R. J., & Barnhart, M. C. (2007b). Chronic toxicity of copper and ammonia to juvenile freshwater mussels (Unionidae). Environmental Toxicology and Chemistry, 26, 2048-2056.
Wang, N., Ingersoll, C. G., Hardesty, D. K., Ivey, C. D., Kunz, J. L., May, T. W., Dwyer, F. J., Roberts, A. D., Augspurger, T., Kane, C. M., Neves, R. J., & Barnhart, M. C. (2007a). Acute toxicity of copper, ammonia, and chlorine to glochidia and juveniles of freshwater mussels (Unionidae). Environmental Toxicology and Chemistry, 26, 2036-2047.
Wang, N., Ingersoll, C. G., Ivey, C. D., Hardesty, D. K., May, T. W., Augspurger, T., Roberts, A. D., van Genderen, E., & Barnhart, M. C. (2010). Sensitivity of early life stages of freshwater mussels (Unionidae) to acute and chronic toxicity of lead, cadmium, and zinc in water. Environmental Toxicology and Chemistry, 29, 2053-2063.
Wang, N., Ivey, C. D., Brunson, E. L., Cleveland, D., Ingersoll, C. G., Stubblefield, W. A., & Cardwell, A. S. (2018a). Acute and chronic toxicity of aluminum to a unionid mussel (Lampsilis siliquoidea) and an amphipod (Hyalella azteca) in water-only exposures. Environmental Toxicology and Chemistry, 37, 61-69.
Wang, N., Ivey, C. D., Dorman, R. A., Ingersoll, C. G., Steevens, J., Hammer, E. J., Bauer, C. R., & Mount, D. R. (2018b). Acute toxicity of sodium chloride and potassium chloride to a unionid mussel (Lampsilis siliquoidea) in water exposures. Environmental Toxicology and Chemistry, 37, 3041-3049.
Wang, N., Ivey, C. D., Ingersoll, C. G., Brumbaugh, W. G., Alvarez, D., Hammer, E. J., Bauer, C. R., Augspurger, T., Raimondo, S., & Barnhart, M. C. (2017). Acute sensitivity of a broad range of freshwater mussels to chemicals with different modes of toxic action. Environmental Toxicology and Chemistry, 36, 786-796.
Wang, N., & Kunz, J. L. (2021). Chemical and biological data from the study on method development for a short-term 7-d toxicity test with unionid mussels. US Geological Survey data release. https://doi.org/10.5066/P93O5K5G
Wang, N., Kunz, J. L., Cleveland, D., Steevens, J. A., & Cozzarelli, I. M. (2019). Biological effects of elevated major ions in surface water contaminated by a produced water from oil production. Archives of Environmental Contamination and Toxicology, 76, 670-677.
Wang, N., Kunz, J. L., Cleveland, D. M., Steevens, J. A., Hammer, E. J., Genderen, E. V., Ryan, A. C., & Schlekat, C. E. (2020b). Evaluation of acute and chronic toxicity of nickel and zinc to 2 sensitive freshwater benthic invertebrates using refined testing methods. Environmental Toxicology and Chemistry, 39, 2256-2268.
Wang, N., Kunz, J. L., Dorman, R. A., Ingersoll, C. G., Steevens, J., Hammer, E. J., & Bauer, C. R. (2018c). Evaluation of chronic toxicity of sodium chloride or potassium chloride to a unionid mussel (Lampsilis siliquoidea) in water exposures using standard and refined toxicity testing methods. Environmental Toxicology and Chemistry, 37, 3050-3062.
Wang, N., Mebane, C. A., Kunz, J. L., Ingersoll, C. G., Brumbaugh, W. G., Santore, R. C., Gorsuch, J. W., & Arnold, W. R. (2011b). Influence of dissolved organic carbon on toxicity of copper to a unionid mussel (Villosa iris) and a cladoceran (Ceriodaphnia dubia) in acute and chronic water exposures. Environmental Toxicology and Chemistry, 30, 2115-2125.