The hydrophobic tail of a pH-sensitive cationic lipid influences siRNA transfection activity and toxicity in human NK cell lines.
Lipid nanoparticle
NK cell
NK-92
Non-viral
pH-sensitive cationic lipid
siRNA
Journal
International journal of pharmaceutics
ISSN: 1873-3476
Titre abrégé: Int J Pharm
Pays: Netherlands
ID NLM: 7804127
Informations de publication
Date de publication:
20 Nov 2021
20 Nov 2021
Historique:
received:
16
06
2021
revised:
23
09
2021
accepted:
24
09
2021
pubmed:
1
10
2021
medline:
10
11
2021
entrez:
30
9
2021
Statut:
ppublish
Résumé
The use of natural killer (NK) cells in cell therapy is an attractive next generation strategy for cancer immunotherapy. NK-92 cells (a human NK cell line) have been tested in clinical trial stages, making them an off-the-shelf medicine. Controlling gene expression in NK-92 cells by an artificial delivery system is an available for enhancing NK-92 cell therapy. We report here on the development of a siRNA-loaded lipid nanoparticle (LNP) composed of CL1H6 (CL1H6-LNP), an optimized, pH-sensitive cationic lipid, with efficient gene silencing and low cytotoxicity in NK-92 cells. The hydrophilic head group of the lipid molecule used in preparing these particles largely influences the pK
Identifiants
pubmed: 34592399
pii: S0378-5173(21)00946-7
doi: 10.1016/j.ijpharm.2021.121140
pii:
doi:
Substances chimiques
Lipids
0
RNA, Small Interfering
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
121140Informations de copyright
Copyright © 2021 Elsevier B.V. All rights reserved.