Linker Threonine-phosphorylated Smad2/3 Is a Biomarker of Colorectal Neoplastic Stem-like Cells that Correlates With Carcinogenesis.
Biomarkers, Tumor
/ metabolism
Carcinogenesis
/ metabolism
Colorectal Neoplasms
/ metabolism
Humans
Intestinal Mucosa
/ metabolism
Neoplastic Stem Cells
/ metabolism
Phosphorylation
Smad2 Protein
/ metabolism
Smad3 Protein
/ metabolism
Threonine
/ metabolism
Tumor Suppressor Protein p53
/ metabolism
Phosphorylation
Smad protein
cancer stem cell
carcinogenesis
colorectal neoplasm
Journal
Anticancer research
ISSN: 1791-7530
Titre abrégé: Anticancer Res
Pays: Greece
ID NLM: 8102988
Informations de publication
Date de publication:
Oct 2021
Oct 2021
Historique:
received:
31
07
2021
revised:
21
08
2021
accepted:
23
08
2021
entrez:
1
10
2021
pubmed:
2
10
2021
medline:
9
10
2021
Statut:
ppublish
Résumé
This study analysed threonine-phosphorylated Smad2/3 (pSmad2/3L-Thr) expression and investigated whether pSmad2/3L-Thr is related to the transition from human colorectal adenoma (CRA) to carcinoma (CRC). Immunofluorescent staining was performed forβ-catenin, p53, CDK4, Ki67, Sox9, aldehyde dehydrogenase (ALDH) 1, and pSmad2/3L-Thr. We analysed specimens of diffuse p53-positive CRCs arising from p53-negative CRAs. Percentage of p53, nuclear β-catenin, Ki67, CDK4, and pSmad2/3L-Thr-positive cells at the site of CRCs was significantly higher than that at the site of CRAs. At the site of normal colorectal mucosae, few epithelial cells were stained positively for pSmad2/3L-Thr. At the site of CRCs, pSmad2/3L-Thr-positive cells showed co-localization with p53, nuclear β-catenin, and ALDH1. At any site, pSmad2/3L-Thr-positive cells showed co-localization with CDK4. pSmad2/3L-Thr correlates with human CRC carcinogenesis, and pSmad2/3L-Thr-positive cells show human colorectal stem cell-like and cancer stem cell characteristics.
Sections du résumé
BACKGROUND/AIM
OBJECTIVE
This study analysed threonine-phosphorylated Smad2/3 (pSmad2/3L-Thr) expression and investigated whether pSmad2/3L-Thr is related to the transition from human colorectal adenoma (CRA) to carcinoma (CRC).
MATERIALS AND METHODS
METHODS
Immunofluorescent staining was performed forβ-catenin, p53, CDK4, Ki67, Sox9, aldehyde dehydrogenase (ALDH) 1, and pSmad2/3L-Thr.
RESULTS
RESULTS
We analysed specimens of diffuse p53-positive CRCs arising from p53-negative CRAs. Percentage of p53, nuclear β-catenin, Ki67, CDK4, and pSmad2/3L-Thr-positive cells at the site of CRCs was significantly higher than that at the site of CRAs. At the site of normal colorectal mucosae, few epithelial cells were stained positively for pSmad2/3L-Thr. At the site of CRCs, pSmad2/3L-Thr-positive cells showed co-localization with p53, nuclear β-catenin, and ALDH1. At any site, pSmad2/3L-Thr-positive cells showed co-localization with CDK4.
CONCLUSION
CONCLUSIONS
pSmad2/3L-Thr correlates with human CRC carcinogenesis, and pSmad2/3L-Thr-positive cells show human colorectal stem cell-like and cancer stem cell characteristics.
Identifiants
pubmed: 34593428
pii: 41/10/4789
doi: 10.21873/anticanres.15294
doi:
Substances chimiques
Biomarkers, Tumor
0
SMAD2 protein, human
0
SMAD3 protein, human
0
Smad2 Protein
0
Smad3 Protein
0
TP53 protein, human
0
Tumor Suppressor Protein p53
0
Threonine
2ZD004190S
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
4789-4799Informations de copyright
Copyright © 2021 International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.