Feasibility of a surveillance programme based on gargle samples and pool testing to prevent SARS-CoV-2 outbreaks in schools.
Adolescent
COVID-19
/ diagnosis
COVID-19 Nucleic Acid Testing
/ statistics & numerical data
Child
Disease Outbreaks
/ prevention & control
Epidemiological Monitoring
Feasibility Studies
Germany
/ epidemiology
Humans
Pandemics
Saliva
/ virology
Schools
/ statistics & numerical data
Students
/ statistics & numerical data
Surveys and Questionnaires
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
30 09 2021
30 09 2021
Historique:
received:
27
05
2021
accepted:
15
09
2021
entrez:
1
10
2021
pubmed:
2
10
2021
medline:
21
10
2021
Statut:
epublish
Résumé
School closures have a negative impact on physical and mental well-being, and education, of children and adolescents. A surveillance programme to detect asymptomatic SARS-CoV-2 infection could allow schools to remain open, while protecting the vulnerable. We assessed the feasibility of a programme employing gargle samples and pool testing of individually extracted RNA using rRT-qPCR in a primary and a secondary school in Germany, based on programme logistics and acceptance. Twice a week, five participants per class were selected to provide samples, using an algorithm weighted by a risk-based priority score to increase likelihood of case detection. The positive response rate was 54.8% (550 of 1003 pupils). Logistics evaluation revealed the rate-limiting steps: completing the regular pre-test questionnaire and handing in the samples. Acceptance questionnaire responses indicated strong support for research into developing a surveillance programme and a positive evaluation of gargle tests. Participation was voluntary. As not all pupils participated, individual reminders could lead to participant identification. School-wide implementation of the programme for infection monitoring purposes would enable reminders to be given to all school pupils to address these steps, without compromising participant anonymity. Such a programme would provide a feasible means to monitor asymptomatic respiratory tract infection in schools.
Identifiants
pubmed: 34593887
doi: 10.1038/s41598-021-98849-1
pii: 10.1038/s41598-021-98849-1
pmc: PMC8484445
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
19521Informations de copyright
© 2021. The Author(s).
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