Experimental evolution supports the potential of neonicotinoid-pyrethroid combination for managing insecticide resistance in malaria vectors.
Animals
Anopheles
/ drug effects
Cytochrome P-450 Enzyme System
/ chemistry
Dose-Response Relationship, Drug
Drug Combinations
Insecticide Resistance
/ drug effects
Insecticides
/ chemistry
Malaria
/ transmission
Models, Molecular
Molecular Conformation
Mosquito Vectors
/ drug effects
Mutation
Neonicotinoids
/ chemistry
Parasitic Sensitivity Tests
Polymorphism, Genetic
Protein Binding
Pyrethrins
/ chemistry
Structure-Activity Relationship
Transcription, Genetic
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
30 09 2021
30 09 2021
Historique:
received:
29
06
2021
accepted:
20
09
2021
entrez:
1
10
2021
pubmed:
2
10
2021
medline:
29
12
2021
Statut:
epublish
Résumé
The introduction of neonicotinoids for managing insecticide resistance in mosquitoes is of high interest as they interact with a biochemical target not previously used in public health. In this concern, Bayer developed a combination of the neonicotinoid clothianidin and the pyrethroid deltamethrin (brand name Fludora Fusion) as a new vector control tool. Although this combination proved to be efficient against pyrethroid-resistant mosquitoes, its ability to prevent the selection of pyrethroid and neonicotinoid resistance alleles was not investigated. In this context, the objective of this work was to study the dynamics and the molecular mechanisms of resistance of An. gambiae to the separated or combined components of this combination. A field-derived An. gambiae line carrying resistance alleles to multiple insecticides at low frequencies was used as a starting for 33 successive generations of controlled selection. Resistance levels to each insecticide and target site mutation frequencies were monitored throughout the selection process. Cross resistance to other public health insecticides were also investigated. RNA-seq was used to compare gene transcription variations and polymorphisms across all lines. This study confirmed the potential of this insecticide combination to impair the selection of resistance as compared to its two separated components. Deltamethrin selection led to the rapid enrichment of the kdr L1014F target-site mutation. Clothianidin selection led to the over-transcription of multiple cytochrome P450s including some showing high homology with those conferring neonicotinoid resistance in other insects. A strong selection signature associated with clothianidin selection was also observed on a P450 gene cluster previously associated with resistance. Within this cluster, the gene CYP6M1 showed the highest selection signature together with a transcription profile supporting a role in clothianidin resistance. Modelling the impact of point mutations selected by clothianidin on CYP6M1 protein structure showed that selection retained a protein variant with a modified active site potentially enhancing clothianidin metabolism. In the context of the recent deployment of neonicotinoids for mosquito control and their frequent usage in agriculture, the present study highlights the benefit of combining them with other insecticides for preventing the selection of resistance and sustaining vector control activities.
Identifiants
pubmed: 34593941
doi: 10.1038/s41598-021-99061-x
pii: 10.1038/s41598-021-99061-x
pmc: PMC8484614
doi:
Substances chimiques
Drug Combinations
0
Insecticides
0
Neonicotinoids
0
Pyrethrins
0
Cytochrome P-450 Enzyme System
9035-51-2
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
19501Informations de copyright
© 2021. The Author(s).
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