Effect of enterocins against methicillin-resistant animal-derived staphylococci.
Animals
Enterocins
Inhibition
MRSA
Journal
Veterinary research communications
ISSN: 1573-7446
Titre abrégé: Vet Res Commun
Pays: Switzerland
ID NLM: 8100520
Informations de publication
Date de publication:
Dec 2021
Dec 2021
Historique:
received:
07
05
2021
accepted:
25
09
2021
pubmed:
3
10
2021
medline:
15
12
2021
entrez:
2
10
2021
Statut:
ppublish
Résumé
The occurence and spread of animal-derived methicillin resistant staphylococci (MRS) worldwide is a current problem, especially due to their increasing incidence in food animals and their products, with possible contamination of food consumers and handlers. Staphylococci isolated from animals (n = 123) were identified with MALDI-TOF mass spectrometry and screened for methicillin/oxacillin/cefoxitin resistance (MR) using the disk diffusion method. Twenty-three phenotypically MRS strains were analysed using PBP2' Latex Agglutination Test Kit to confirm the phenotypic MR and PCR was performed for mecA gene detection; mecA gene positive strains were furtherly confirmed by means of sequencing. The susceptibility of MRS to 11 partially-purified enterocins (Ent) produced by E. faecium, E. durans and E. mundtii strains of animal, feed/food and environmental origin was checked using agar spot tests. Out of 23 MRS, PBP testing confirmed MR in 17 strains. Three Staphylococcus epidermidis and one S. vitulinus were mecA positive. The majority of MRS, including two mecA gene-positive strains S. epidermidis R44/1 and P3/Tr2a, were susceptible to the tested enterocins, mainly to Ent7420, EntA(P)/EK13, Ent412, Ent55 and Ent9296 (in the range 100 - 12,800 AU/mL). The most susceptible strains appeared to be the mecA gene-positive S. epidermidis SE R44/1 and SE P3/Tr2a strains, inhibited by eight enterocins out of 11 tested (100-200 AU/mL). Only four strains (including mecA gene positive S. epidermidis SE P3/Tr1 and S. vitulinus SV K12PL/1) were resistant to the tested antimicrobial substances. These results indicate that the enterocins used offer a promising option for prevention and treatment of bacterial infection caused by MRS in animals.
Identifiants
pubmed: 34599692
doi: 10.1007/s11259-021-09841-z
pii: 10.1007/s11259-021-09841-z
doi:
Substances chimiques
Anti-Bacterial Agents
0
Bacteriocins
0
Bridged-Ring Compounds
0
enterocin
59678-46-5
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
467-473Subventions
Organisme : Agentúra Ministerstva Školstva, Vedy, Výskumu a Športu SR
ID : VEGA 2/0005/21
Informations de copyright
© 2021. The Author(s), under exclusive licence to Springer Nature B.V.
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