Malaria parasite species composition of Plasmodium infections among asymptomatic and symptomatic school-age children in rural and urban areas of Kinshasa, Democratic Republic of Congo.
Democratic Republic Congo
Malaria
Plasmodium
School-age children
Journal
Malaria journal
ISSN: 1475-2875
Titre abrégé: Malar J
Pays: England
ID NLM: 101139802
Informations de publication
Date de publication:
02 Oct 2021
02 Oct 2021
Historique:
accepted:
16
09
2021
entrez:
3
10
2021
pubmed:
4
10
2021
medline:
13
1
2022
Statut:
epublish
Résumé
Malaria remains a major public health concern in the Democratic Republic of Congo (DRC), and school-age children are relatively neglected in malaria prevalence surveys and may constitute a significant reservoir of transmission. This study aimed to understand the burden of malaria infections in school-age children in Kinshasa/DRC. A total of 634 (427 asymptomatic and 207 symptomatic) blood samples collected from school-age children aged 6 to 14 years were analysed by microscopy, RDT and Nested-PCR. The overall prevalence of Plasmodium spp. by microscopy, RDT and PCR was 33%, 42% and 62% among asymptomatic children and 59%, 64% and 95% in symptomatic children, respectively. The prevalence of Plasmodium falciparum, Plasmodium malariae and Plasmodium ovale spp. by PCR was 58%, 20% and 11% among asymptomatic and 93%, 13% and 16% in symptomatic children, respectively. Among P. ovale spp., P. ovale curtisi, P. ovale wallikeri and mixed P. ovale curtisi + P. ovale wallikeri accounted for 75%, 24% and 1% of infections, respectively. All Plasmodium species infections were significantly more prevalent in the rural area compared to the urban area in asymptomatic infections (p < 0.001). Living in a rural as opposed to an urban area was associated with a five-fold greater risk of asymptomatic malaria parasite carriage (p < 0.001). Amongst asymptomatic malaria parasite carriers, 43% and 16% of children harboured mixed Plasmodium with P. falciparum infections in the rural and the urban areas, respectively, whereas in symptomatic malaria infections, it was 22% and 26%, respectively. Few children carried single infections of P. malariae (2.2%) and P. ovale spp. (1.9%). School-age children are at significant risk from both asymptomatic and symptomatic malaria infections. Continuous systematic screening and treatment of school-age children in high-transmission settings is needed.
Sections du résumé
BACKGROUND
BACKGROUND
Malaria remains a major public health concern in the Democratic Republic of Congo (DRC), and school-age children are relatively neglected in malaria prevalence surveys and may constitute a significant reservoir of transmission. This study aimed to understand the burden of malaria infections in school-age children in Kinshasa/DRC.
METHODS
METHODS
A total of 634 (427 asymptomatic and 207 symptomatic) blood samples collected from school-age children aged 6 to 14 years were analysed by microscopy, RDT and Nested-PCR.
RESULTS
RESULTS
The overall prevalence of Plasmodium spp. by microscopy, RDT and PCR was 33%, 42% and 62% among asymptomatic children and 59%, 64% and 95% in symptomatic children, respectively. The prevalence of Plasmodium falciparum, Plasmodium malariae and Plasmodium ovale spp. by PCR was 58%, 20% and 11% among asymptomatic and 93%, 13% and 16% in symptomatic children, respectively. Among P. ovale spp., P. ovale curtisi, P. ovale wallikeri and mixed P. ovale curtisi + P. ovale wallikeri accounted for 75%, 24% and 1% of infections, respectively. All Plasmodium species infections were significantly more prevalent in the rural area compared to the urban area in asymptomatic infections (p < 0.001). Living in a rural as opposed to an urban area was associated with a five-fold greater risk of asymptomatic malaria parasite carriage (p < 0.001). Amongst asymptomatic malaria parasite carriers, 43% and 16% of children harboured mixed Plasmodium with P. falciparum infections in the rural and the urban areas, respectively, whereas in symptomatic malaria infections, it was 22% and 26%, respectively. Few children carried single infections of P. malariae (2.2%) and P. ovale spp. (1.9%).
CONCLUSION
CONCLUSIONS
School-age children are at significant risk from both asymptomatic and symptomatic malaria infections. Continuous systematic screening and treatment of school-age children in high-transmission settings is needed.
Identifiants
pubmed: 34600558
doi: 10.1186/s12936-021-03919-4
pii: 10.1186/s12936-021-03919-4
pmc: PMC8487491
doi:
Substances chimiques
DNA, Protozoan
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
389Informations de copyright
© 2021. The Author(s).
Références
Clin Microbiol Rev. 2005 Jul;18(3):570-81
pubmed: 16020691
Malar J. 2014 Nov 18;13:433
pubmed: 25404207
Clin Infect Dis. 2011 May;52(9):1100-7
pubmed: 21467015
Antimicrob Agents Chemother. 2012 Oct;56(10):5271-7
pubmed: 22850519
Am J Trop Med Hyg. 1971 Nov;20(6):825-7
pubmed: 5131690
Malar J. 2016 Jan 13;15:27
pubmed: 26762532
Parasitol Int. 2018 Feb;67(1):29-33
pubmed: 28263883
PLoS Negl Trop Dis. 2019 May 1;13(5):e0007290
pubmed: 31042707
Malar J. 2012 Jan 31;11:29
pubmed: 22289302
Malar J. 2004 Jun 17;3:18
pubmed: 15202944
Soc Sci Med. 2010 Jul;71(2):324-334
pubmed: 20413198
Front Immunol. 2020 Jan 22;10:3072
pubmed: 32038623
Rev Inst Med Trop Sao Paulo. 2011 Jan-Feb;53(1):55-9
pubmed: 21412621
Sci Rep. 2021 Mar 19;11(1):6409
pubmed: 33742015
Malar J. 2008 Jan 23;7:17
pubmed: 18215251
Trans R Soc Trop Med Hyg. 2000 Sep-Oct;94(5):472-6
pubmed: 11132369
Am J Trop Med Hyg. 2002 May;66(5):492-502
pubmed: 12201582
J Clin Microbiol. 2011 Aug;49(8):2946-53
pubmed: 21653767
BMC Public Health. 2009 Oct 01;9:369
pubmed: 19796380
Parasitol Today. 1998 Oct;14(10):428-34
pubmed: 17040835
Elife. 2018 Jul 25;7:
pubmed: 30044224
Am J Trop Med Hyg. 1954 Jul;3(4):628-37
pubmed: 13180821
Trans R Soc Trop Med Hyg. 1955 Mar;49(2):158-67
pubmed: 14373852
Clin Infect Dis. 2005 Nov 15;41(10):1544-5
pubmed: 16231275
Int J Parasitol Drugs Drug Resist. 2013 Jan 19;3:45-50
pubmed: 24533292
Lancet. 2008 Jul 12;372(9633):127-38
pubmed: 18620950
Am J Trop Med Hyg. 2017 Sep;97(3):753-757
pubmed: 28990911
Malar J. 2016 Feb 11;15:81
pubmed: 26864461
Clin Infect Dis. 2019 Nov 27;69(12):2119-2126
pubmed: 31066448
Am J Trop Med Hyg. 2004 Aug;71(2 Suppl):97-102
pubmed: 15331825
Malar J. 2017 Oct 3;16(1):398
pubmed: 28974215
Int J Parasitol. 2012 Jun;42(7):693-9
pubmed: 22633951
Malar J. 2013 Sep 28;12:346
pubmed: 24073668
Parasitol Int. 2015 Jun;64(3):304-8
pubmed: 25256904
PLoS Negl Trop Dis. 2015 Dec 31;9(12):e0004195
pubmed: 26720002
Parasitol Today. 1987 Jul;3(7):206-14
pubmed: 15462957
Malar J. 2010 Jan 04;9:2
pubmed: 20044942
Lancet. 1994 Apr 30;343(8905):1095
pubmed: 7909108
Semin Nephrol. 2003 Jan;23(1):21-33
pubmed: 12563598
J Med Entomol. 2005 Sep;42(5):777-9
pubmed: 16363160
Clin Microbiol Infect. 2011 Mar;17(3):469-75
pubmed: 20298268
Malar J. 2017 Mar 11;16(1):112
pubmed: 28284211
Soc Sci Med. 2010 Jul;71(2):314-323
pubmed: 20471149
Lancet Infect Dis. 2006 Dec;6(12):780-93
pubmed: 17123898
Proc Natl Acad Sci U S A. 2010 Mar 30;107(13):5967-71
pubmed: 20231434
Lancet. 1991 Oct 5;338(8771):896
pubmed: 1681259
Open Forum Infect Dis. 2014 Jun 30;1(1):ofu034
pubmed: 25734104
Am J Trop Med Hyg. 2013 Jun;88(6):1102-1108
pubmed: 23589533
Trans R Soc Trop Med Hyg. 1996 Jan-Feb;90(1):15-9
pubmed: 8730301
Lancet. 1997 Jun 7;349(9066):1650-4
pubmed: 9186382
Acta Trop. 2010 Dec;116(3):167-72
pubmed: 20727338
Am J Trop Med Hyg. 2018 Nov;99(5):1128-1133
pubmed: 30203741
Malar J. 2013 Nov 04;12:389
pubmed: 24180319
J Infect Dis. 2010 May 15;201(10):1544-50
pubmed: 20380562
J Infect. 1991 Nov;23(3):343-4
pubmed: 1753151
Clin Microbiol Rev. 2007 Oct;20(4):579-92
pubmed: 17934075
Elife. 2018 Aug 21;7:
pubmed: 30129437
J Infect Dis. 2009 Nov 1;200(9):1465-9
pubmed: 19803728
Presse Med. 2005 Mar 12;34(5):371-2
pubmed: 15859572
J Infect Dis. 2009 Jul 1;200(1):66-74
pubmed: 19476434
Malar J. 2014 May 03;13:170
pubmed: 24886496
Mol Biochem Parasitol. 1993 Apr;58(2):283-92
pubmed: 8479452
Med Vet Entomol. 1993 Apr;7(2):127-37
pubmed: 8481529
Malar J. 2010 Dec 20;9:366
pubmed: 21171998
J Travel Med. 2011 Jul-Aug;18(4):288-91
pubmed: 21722243
Trop Med Int Health. 2000 Jan;5(1):3-8
pubmed: 10672199
Immunol Today. 1991 Mar;12(3):A68-71
pubmed: 2069680
PLoS Negl Trop Dis. 2015 Jan 15;9(1):e0003469
pubmed: 25590587
Am J Trop Med Hyg. 2006 Oct;75(4):588-96
pubmed: 17038678
Int J Parasitol. 2011 May;41(6):677-83
pubmed: 21315074
Parasitology. 2014 Dec;141(14):1880-90
pubmed: 24837880
Trans R Soc Trop Med Hyg. 1987;81(3):478-86
pubmed: 3318021
Malar J. 2018 Sep 20;17(1):334
pubmed: 30236117
Malar J. 2016 Jul 08;15:350
pubmed: 27392905
Int J Parasitol. 2021 Aug 11;:
pubmed: 34390743
Sci Rep. 2019 Jul 5;9(1):9784
pubmed: 31278305
PLoS One. 2008 Jul 23;3(7):e2775
pubmed: 18648666
Malar J. 2013 Dec 05;12:439
pubmed: 24304475