Distinct B cell subsets in Peyer's patches convey probiotic effects by Limosilactobacillus reuteri.
Gut microbiome
Inflammatory bowel disease
Innate-like B lymphocytes
PD-1 dependent
Probiotics
R2LC
Journal
Microbiome
ISSN: 2049-2618
Titre abrégé: Microbiome
Pays: England
ID NLM: 101615147
Informations de publication
Date de publication:
03 10 2021
03 10 2021
Historique:
received:
28
01
2021
accepted:
01
07
2021
entrez:
4
10
2021
pubmed:
5
10
2021
medline:
25
2
2023
Statut:
epublish
Résumé
Intestinal Peyer's patches (PPs) form unique niches for bacteria-immune cell interactions that direct host immunity and shape the microbiome. Here we investigate how peroral administration of probiotic bacterium Limosilactobacillus reuteri R2LC affects B lymphocytes and IgA induction in the PPs, as well as the downstream consequences on intestinal microbiota and susceptibility to inflammation. The B cells of PPs were separated by size to circumvent activation-dependent cell identification biases due to dynamic expression of markers, which resulted in two phenotypically, transcriptionally, and spatially distinct subsets: small IgD The Peyer's patches sense, enhance and transmit probiotic signals by increasing the numbers and effector functions of distinct B cell subsets, resulting in increased IgA production, altered intestinal microbiota, and protection against inflammation. Video abstract.
Sections du résumé
BACKGROUND
Intestinal Peyer's patches (PPs) form unique niches for bacteria-immune cell interactions that direct host immunity and shape the microbiome. Here we investigate how peroral administration of probiotic bacterium Limosilactobacillus reuteri R2LC affects B lymphocytes and IgA induction in the PPs, as well as the downstream consequences on intestinal microbiota and susceptibility to inflammation.
RESULTS
The B cells of PPs were separated by size to circumvent activation-dependent cell identification biases due to dynamic expression of markers, which resulted in two phenotypically, transcriptionally, and spatially distinct subsets: small IgD
CONCLUSIONS
The Peyer's patches sense, enhance and transmit probiotic signals by increasing the numbers and effector functions of distinct B cell subsets, resulting in increased IgA production, altered intestinal microbiota, and protection against inflammation. Video abstract.
Identifiants
pubmed: 34602091
doi: 10.1186/s40168-021-01128-4
pii: 10.1186/s40168-021-01128-4
pmc: PMC8487498
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Video-Audio Media
Langues
eng
Sous-ensembles de citation
IM
Pagination
198Informations de copyright
© 2021. The Author(s).
Références
Science. 2016 May 13;352(6287):aaf4822
pubmed: 27174992
J Bacteriol. 2018 Jun 11;200(13):
pubmed: 29686137
Science. 2005 Sep 9;309(5741):1735-9
pubmed: 16151014
Nat Rev Drug Discov. 2010 Nov;9(11):883-97
pubmed: 21031003
Immunity. 2000 Oct;13(4):443-51
pubmed: 11070163
Immunity. 2015 Sep 15;43(3):527-40
pubmed: 26362264
Nat Immunol. 2017 Mar;18(3):303-312
pubmed: 28114292
Mucosal Immunol. 2017 Jul;10(4):957-970
pubmed: 27848952
J Exp Med. 2017 Jan;214(1):107-123
pubmed: 27994068
ISME J. 2010 Mar;4(3):377-87
pubmed: 19924154
Annu Rev Immunol. 2018 Apr 26;36:359-381
pubmed: 29400985
Nat Immunol. 2015 Jun;16(6):663-73
pubmed: 25894659
Elife. 2016 Aug 03;5:
pubmed: 27487469
Mucosal Immunol. 2017 Jan;10(1):162-171
pubmed: 27049060
Nature. 2013 Jan 31;493(7434):684-8
pubmed: 23263181
Nature. 2016 Sep 8;537(7619):234-238
pubmed: 27501247
Cell Rep. 2020 Feb 4;30(5):1530-1541.e4
pubmed: 32023467
ACS Synth Biol. 2014 Feb 21;3(2):97-106
pubmed: 24932563
Int J Syst Evol Microbiol. 2020 Apr;70(4):2782-2858
pubmed: 32293557
Front Immunol. 2019 Jun 04;10:1235
pubmed: 31214189
Cancer Cell. 2018 Apr 9;33(4):570-580
pubmed: 29634945
Acta Physiol (Oxf). 2016 Aug;217(4):300-10
pubmed: 27096537
Science. 2017 Aug 25;357(6353):806-810
pubmed: 28775213
Immunity. 2015 Sep 15;43(3):541-53
pubmed: 26320660
Mucosal Immunol. 2017 Nov;10(6):1361-1374
pubmed: 28745325
Curr Opin Immunol. 2018 Oct;54:137-144
pubmed: 30205357
Science. 2017 Oct 20;358(6361):
pubmed: 28971969
Nat Methods. 2013 Dec;10(12):1200-2
pubmed: 24076764
Immunity. 2016 Sep 20;45(3):471-482
pubmed: 27653600
Nat Immunol. 2019 Apr;20(4):482-492
pubmed: 30833793
J Immunol. 2004 Sep 1;173(5):3305-9
pubmed: 15322193
Immunol Rev. 2016 May;271(1):230-45
pubmed: 27088918
Immunity. 2016 Jul 19;45(1):131-44
pubmed: 27421702
Science. 2012 Apr 27;336(6080):485-9
pubmed: 22539724
PLoS One. 2015 Feb 03;10(2):e0116955
pubmed: 25647581
J Immunol Methods. 1983 Sep 30;63(1):45-56
pubmed: 6604756
J Bacteriol. 1995 Dec;177(24):7011-8
pubmed: 8522504
Immunity. 2018 Apr 17;48(4):702-715.e4
pubmed: 29669250
Proc Natl Acad Sci U S A. 2011 Mar 15;108 Suppl 1:4645-52
pubmed: 20615995
Nature. 2015 May 7;521(7550):90-93
pubmed: 25686606
Front Microbiol. 2018 Apr 19;9:757
pubmed: 29725324
Science. 2002 Apr 12;296(5566):346-9
pubmed: 11923495
J Leukoc Biol. 2009 May;85(5):744-50
pubmed: 19168593
Front Immunol. 2019 Mar 07;10:385
pubmed: 30899262
Antonie Van Leeuwenhoek. 1992 Apr;61(3):167-73
pubmed: 1519914
Microbiol Resour Announc. 2019 Apr 4;8(14):
pubmed: 30948464
Nat Commun. 2019 Jun 3;10(1):2423
pubmed: 31160559
Blood. 2013 Oct 10;122(15):2591-9
pubmed: 23823318