Isolation of Myofibres and Culture of Muscle Stem Cells from Adult Zebrafish.
Adult
MuSC
Muscle fibre
Myofibre
Myonucleus
Pax7
Satellite cell
Skeletal muscle
Stem cell
Zebrafish
Journal
Bio-protocol
ISSN: 2331-8325
Titre abrégé: Bio Protoc
Pays: United States
ID NLM: 101635102
Informations de publication
Date de publication:
05 Sep 2021
05 Sep 2021
Historique:
received:
19
03
2021
revised:
24
05
2021
accepted:
01
06
2021
entrez:
4
10
2021
pubmed:
5
10
2021
medline:
5
10
2021
Statut:
epublish
Résumé
Skeletal muscles generate force throughout life and require maintenance and repair to ensure efficiency. The population of resident muscle stem cells (MuSCs), termed satellite cells, dwells beneath the basal lamina of adult myofibres and contributes to both muscle growth and regeneration. Upon exposure to activating signals, MuSCs proliferate to generate myoblasts that differentiate and fuse to grow or regenerate myofibres. This myogenic progression resembles aspects of muscle formation and development during embryogenesis. Therefore, the study of MuSCs and their associated myofibres permits the exploration of muscle stem cell biology, including the cellular and molecular mechanisms underlying muscle formation, maintenance and repair. As most aspects of MuSC biology have been described in rodents, their relevance to other species, including humans, is unclear and would benefit from comparison to an alternative vertebrate system. Here, we describe a procedure for the isolation and immunolabelling or culture of adult zebrafish myofibres that allows examination of both myofibre characteristics and MuSC biology
Identifiants
pubmed: 34604454
doi: 10.21769/BioProtoc.4149
pii: e4149
pmc: PMC8443456
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e4149Subventions
Organisme : Medical Research Council
ID : MR/S002472/1
Pays : United Kingdom
Informations de copyright
Copyright © The Authors; exclusive licensee Bio-protocol LLC.
Déclaration de conflit d'intérêts
Competing interestsThe authors declare that they have no competing interests.
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