Kinetic Analysis of a Protein-protein Complex to Determine its Dissociation Constant (K
EC50
IC50
KD
Binding kinetics
Biolayer interferometry
PII signaling protein
Protein-protein interaction
Journal
Bio-protocol
ISSN: 2331-8325
Titre abrégé: Bio Protoc
Pays: United States
ID NLM: 101635102
Informations de publication
Date de publication:
05 Sep 2021
05 Sep 2021
Historique:
received:
04
03
2021
revised:
07
06
2021
accepted:
21
06
2021
entrez:
4
10
2021
pubmed:
5
10
2021
medline:
5
10
2021
Statut:
epublish
Résumé
Biolayer interferometry (BLI) is an emerging analytical tool that allows the study of protein complexes in real time to determine protein complex kinetic parameters. This article describes a protocol to determine the K
Identifiants
pubmed: 34604457
doi: 10.21769/BioProtoc.4152
pii: e4152
pmc: PMC8443453
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e4152Informations de copyright
Copyright © 2021 The Authors; exclusive licensee Bio-protocol LLC.
Déclaration de conflit d'intérêts
Competing interestsThe authors declare no competing interest.
Références
New Phytol. 2020 Aug;227(3):722-731
pubmed: 32077495
mBio. 2021 Mar 23;12(2):
pubmed: 33758091
J Vis Exp. 2014 Feb 18;(84):e51383
pubmed: 24638157
J Biol Chem. 2020 May 1;295(18):6165-6176
pubmed: 32179648
Proc Natl Acad Sci U S A. 2020 May 26;117(21):11727-11734
pubmed: 32376634
Mol Microbiol. 2020 Aug;114(2):292-307
pubmed: 32274833
Proc Natl Acad Sci U S A. 2021 Feb 9;118(6):
pubmed: 33526690
FEMS Microbiol Rev. 2020 Jan 1;44(1):33-53
pubmed: 31617886