SARS-CoV-2-Reactive Mucosal B Cells in the Upper Respiratory Tract of Uninfected Individuals.
Adenoids
/ immunology
Antibodies, Viral
/ metabolism
B-Lymphocyte Subsets
/ immunology
B-Lymphocytes
/ immunology
COVID-19
/ immunology
Child
HEK293 Cells
Humans
Immunologic Memory
Lymphocyte Activation
Mucous Membrane
/ immunology
Receptors, Antigen, B-Cell
/ genetics
Respiratory System
/ immunology
SARS-CoV-2
/ physiology
Single-Cell Analysis
Spike Glycoprotein, Coronavirus
/ immunology
Transcriptome
Journal
Journal of immunology (Baltimore, Md. : 1950)
ISSN: 1550-6606
Titre abrégé: J Immunol
Pays: United States
ID NLM: 2985117R
Informations de publication
Date de publication:
15 11 2021
15 11 2021
Historique:
received:
22
06
2021
accepted:
02
09
2021
pubmed:
6
10
2021
medline:
16
11
2021
entrez:
5
10
2021
Statut:
ppublish
Résumé
SARS-CoV-2 is a respiratory pathogen that can cause severe disease in at-risk populations but results in asymptomatic infections or a mild course of disease in the majority of cases. We report the identification of SARS-CoV-2-reactive B cells in human tonsillar tissue obtained from children who were negative for coronavirus disease 2019 prior to the pandemic and the generation of mAbs recognizing the SARS-CoV-2 Spike protein from these B cells. These Abs showed reduced binding to Spike proteins of SARS-CoV-2 variants and did not recognize Spike proteins of endemic coronaviruses, but subsets reacted with commensal microbiota and exhibited SARS-CoV-2-neutralizing potential. Our study demonstrates pre-existing SARS-CoV-2-reactive Abs in various B cell populations in the upper respiratory tract lymphoid tissue that may lead to the rapid engagement of the pathogen and contribute to prevent manifestations of symptomatic or severe disease.
Identifiants
pubmed: 34607939
pii: jimmunol.2100606
doi: 10.4049/jimmunol.2100606
doi:
Substances chimiques
Antibodies, Viral
0
Receptors, Antigen, B-Cell
0
Spike Glycoprotein, Coronavirus
0
spike protein, SARS-CoV-2
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2581-2588Informations de copyright
Copyright © 2021 by The American Association of Immunologists, Inc.