A Novel Multifunctional Arabinofuranosidase/Endoxylanase/β-Xylosidase GH43 Enzyme from Paenibacillus curdlanolyticus B-6 and Its Synergistic Action To Produce Arabinose and Xylose from Cereal Arabinoxylan.

Paenibacillus curdlanolyticus carbohydrate-binding module cereal arabinoxylan cereal biomass glycoside hydrolase family 43 multifunctional xylanolytic enzyme α-l-arabinofuranosidase/endoxylanase/β-xylosidase activities

Journal

Applied and environmental microbiology
ISSN: 1098-5336
Titre abrégé: Appl Environ Microbiol
Pays: United States
ID NLM: 7605801

Informations de publication

Date de publication:
24 11 2021
Historique:
pubmed: 7 10 2021
medline: 29 12 2021
entrez: 6 10 2021
Statut: ppublish

Résumé

PcAxy43B is a modular protein comprising a catalytic domain of glycoside hydrolase family 43 (GH43), a family 6 carbohydrate-binding module (CBM6), and a family 36 carbohydrate-binding module (CBM36) and found to be a novel multifunctional xylanolytic enzyme from Paenibacillus curdlanolyticus B-6. This enzyme exhibited α-l-arabinofuranosidase, endoxylanase, and β-d-xylosidase activities. The α-l-arabinofuranosidase activity of PcAxy43B revealed a new property of GH43, via the release of both long-chain cereal arabinoxylan and short-chain arabinoxylooligosaccharide (AXOS), as well as release from both the C(O)

Identifiants

pubmed: 34613758
doi: 10.1128/AEM.01730-21
pmc: PMC8612256
doi:

Substances chimiques

Bacterial Proteins 0
Multifunctional Enzymes 0
Xylans 0
arabinoxylan 9040-27-1
Xylose A1TA934AKO
Arabinose B40ROO395Z
Glycoside Hydrolases EC 3.2.1.-
Xylosidases EC 3.2.1.-
exo-1,4-beta-D-xylosidase EC 3.2.1.37
Endo-1,4-beta Xylanases EC 3.2.1.8

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

e0173021

Subventions

Organisme : Thailand Research Fund
ID : PHD/0106/2553

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Auteurs

Puangpen Limsakul (P)

School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkok, Thailand.

Paripok Phitsuwan (P)

School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkok, Thailand.

Rattiya Waeonukul (R)

School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkok, Thailand.
Excellent Center of Enzyme Technology and Microbial Utilization, Pilot Plant Development and Training Institute, King Mongkut's University of Technology Thonburi, Bangkok, Thailand.

Patthra Pason (P)

School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkok, Thailand.
Excellent Center of Enzyme Technology and Microbial Utilization, Pilot Plant Development and Training Institute, King Mongkut's University of Technology Thonburi, Bangkok, Thailand.

Chakrit Tachaapaikoon (C)

School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkok, Thailand.
Excellent Center of Enzyme Technology and Microbial Utilization, Pilot Plant Development and Training Institute, King Mongkut's University of Technology Thonburi, Bangkok, Thailand.

Kanokwan Poomputsa (K)

School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkok, Thailand.

Akihiko Kosugi (A)

Biological Resources and Post-harvest Division, Japan International Research Center for Agricultural Sciencesgrid.452611.5, Ibaraki, Japan.

Khanok Ratanakhanokchai (K)

School of Bioresources and Technology, King Mongkut's University of Technology Thonburi, Bangkok, Thailand.
Excellent Center of Enzyme Technology and Microbial Utilization, Pilot Plant Development and Training Institute, King Mongkut's University of Technology Thonburi, Bangkok, Thailand.

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Classifications MeSH