Pilot investigation on the dose-dependent impact of irradiation on primary human alveolar osteoblasts in vitro.
Biomarkers
Calcification, Physiologic
/ drug effects
Cell Differentiation
/ radiation effects
Cell Proliferation
/ radiation effects
Cells, Cultured
Dose-Response Relationship, Radiation
Fluorescent Antibody Technique
Humans
Osteoblasts
/ cytology
Pilot Projects
Protein Biosynthesis
Radiation, Ionizing
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
06 10 2021
06 10 2021
Historique:
received:
28
06
2021
accepted:
20
09
2021
entrez:
7
10
2021
pubmed:
8
10
2021
medline:
31
12
2021
Statut:
epublish
Résumé
Radiotherapy of head and neck squamous cell carcinoma can lead to long-term complications like osteoradionecrosis, resulting in severe impairment of the jawbone. Current standard procedures require a 6-month wait after irradiation before dental reconstruction can begin. A comprehensive characterization of the irradiation-induced molecular and functional changes in bone cells could allow the development of novel strategies for an earlier successful dental reconstruction in patients treated by radiotherapy. The impact of ionizing radiation on the bone-forming alveolar osteoblasts remains however elusive, as previous studies have relied on animal-based models and fetal or animal-derived cell lines. This study presents the first in vitro data obtained from primary human alveolar osteoblasts. Primary human alveolar osteoblasts were isolated from healthy donors and expanded. After X-ray irradiation with 2, 6 and 10 Gy, cells were cultivated under osteogenic conditions and analyzed regarding their proliferation, mineralization, and expression of marker genes and proteins. Proliferation of osteoblasts decreased in a dose-dependent manner. While cells recovered from irradiation with 2 Gy, application of 6 and 10 Gy doses not only led to a permanent impairment of proliferation, but also resulted in altered cell morphology and a disturbed structure of the extracellular matrix as demonstrated by immunostaining of collagen I and fibronectin. Following irradiation with any of the examined doses, a decrease of marker gene expression levels was observed for most of the investigated genes, revealing interindividual differences. Primary human alveolar osteoblasts presented a considerably changed phenotype after irradiation, depending on the dose administered. Mechanisms for these findings need to be further investigated. This could facilitate improved patient care by re-evaluating current standard procedures and investigating faster and safer reconstruction concepts, thus improving quality of life and social integrity.
Identifiants
pubmed: 34615948
doi: 10.1038/s41598-021-99323-8
pii: 10.1038/s41598-021-99323-8
pmc: PMC8494843
doi:
Substances chimiques
Biomarkers
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
19833Informations de copyright
© 2021. The Author(s).
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