The Regional Specificity of Mucosa-Associated Microbiota in Patients with Distal Colorectal Cancer.

Colorectal Neoplasms / pathology Fusobacterium nucleatum / genetics Humans Microbiota Mucous Membrane / pathology RNA, Ribosomal, 16S / genetics Tumor Microenvironment
Colorectal cancer Fusobacterium Intestinal microbiota Peptostreptococcus Streptococcus

Journal

Digestion
ISSN: 1421-9867
Titre abrégé: Digestion
Pays: Switzerland
ID NLM: 0150472

Informations de publication

Date de publication:
2022
Historique:
received: 03 06 2021
accepted: 06 09 2021
pubmed: 8 10 2021
medline: 3 3 2022
entrez: 7 10 2021
Statut: ppublish

Résumé

Recent studies have demonstrated that the populations of several microbes are significantly increased in fecal samples from patients with colorectal cancer (CRC), suggesting their involvement in the development of CRC. The aim of this study was to identify microbes which are increased in distal CRCs and to identify the specific location of microbes increased in mucosal tissue around the tumor. Tissue specimens were collected from surgical resections of 28 distal CRCs. Five samples were collected from each specimen (location A: tumor, B: adjacent normal mucosa, C: normal mucosa 1 cm proximal to the tumor, D: normal mucosa 3 cm proximally, and E: normal mucosa 6 cm proximally). The microbiota in the sample were analyzed using 16S rRNA gene amplicon sequencing and the relative abundance (RA) of microbiota compared among the 5 locations. At the genus level, the RA of Fusobacterium and Streptococcus at location A was the highest among the 5 locations, significantly different from that in location E. The dominant species of each genus was Fusobacterium nucleatum and Streptococcus anginosus. The RAs of these species gradually decreased from locations B to E with a statistically significant difference in F. nucleatum. The genus Peptostreptococcus also showed a similar trend, and the RA of Peptostreptococcus stomatis in location A was significantly associated with depth of tumor invasion and tumor size. Although the clinical relevance is not clear yet, these results suggest that F. nucleatum, S. anginosus, and P. stomatis can spread to the adjacent normal tissues and may change the surrounding microenvironment to support the progression of CRC.

Sections du résumé

BACKGROUND/AIMS OBJECTIVE
Recent studies have demonstrated that the populations of several microbes are significantly increased in fecal samples from patients with colorectal cancer (CRC), suggesting their involvement in the development of CRC. The aim of this study was to identify microbes which are increased in distal CRCs and to identify the specific location of microbes increased in mucosal tissue around the tumor.
METHODS METHODS
Tissue specimens were collected from surgical resections of 28 distal CRCs. Five samples were collected from each specimen (location A: tumor, B: adjacent normal mucosa, C: normal mucosa 1 cm proximal to the tumor, D: normal mucosa 3 cm proximally, and E: normal mucosa 6 cm proximally). The microbiota in the sample were analyzed using 16S rRNA gene amplicon sequencing and the relative abundance (RA) of microbiota compared among the 5 locations.
RESULTS RESULTS
At the genus level, the RA of Fusobacterium and Streptococcus at location A was the highest among the 5 locations, significantly different from that in location E. The dominant species of each genus was Fusobacterium nucleatum and Streptococcus anginosus. The RAs of these species gradually decreased from locations B to E with a statistically significant difference in F. nucleatum. The genus Peptostreptococcus also showed a similar trend, and the RA of Peptostreptococcus stomatis in location A was significantly associated with depth of tumor invasion and tumor size.
CONCLUSION CONCLUSIONS
Although the clinical relevance is not clear yet, these results suggest that F. nucleatum, S. anginosus, and P. stomatis can spread to the adjacent normal tissues and may change the surrounding microenvironment to support the progression of CRC.

Identifiants

DOI: 10.1159/000519487 PMID: 34619680
pubmed: 34619680
pii: 000519487
doi: 10.1159/000519487
doi:

Substances chimiques

RNA, Ribosomal, 16S 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

141-149

Informations de copyright

© 2021 S. Karger AG, Basel.

Auteurs

Yoshihiko Kono (Y)

Department of Surgery, Jichi Medical University, Shimotsuke, Japan.

Ryo Inoue (R)

Laboratory of Animal Science, Department of Applied Biological Sciences, Faculty of Agriculture, Setsunan University, Osaka, Japan.

Takumi Teratani (T)

Center for Development of Advanced Technology, Jichi Medical University, Shimotsuke, Japan.

Mineyuki Tojo (M)

Department of Surgery, Jichi Medical University, Shimotsuke, Japan.

Yuko Kumagai (Y)

Department of Surgery, Jichi Medical University, Shimotsuke, Japan.

So Morishima (S)

Department of Agriculture and Life Science, Kyoto Prefectural University, Kyoto, Japan.

Koji Koinuma (K)

Department of Surgery, Jichi Medical University, Shimotsuke, Japan.

Alan Kawarai Lefor (AK)

Department of Surgery, Jichi Medical University, Shimotsuke, Japan.

Joji Kitayama (J)

Department of Surgery, Jichi Medical University, Shimotsuke, Japan.

Naohiro Sata (N)

Department of Surgery, Jichi Medical University, Shimotsuke, Japan.

Hisanaga Horie (H)

Department of Surgery, Jichi Medical University, Shimotsuke, Japan.
Department of Operating Room Management, Jichi Medical University Hospital, Shimotsuke, Japan.

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Classifications MeSH

questionsmedicales.fr › Maladies de l'appareil digestif › Maladies gastro-intestinales › Maladies intestinales › Maladies du rectum › Tumeurs colorectales › The Regional Specificity of Mucosa-Associated...
questionsmedicales.fr › Bactéries › Bactéries à Gram négatif › Bactéries anaérobies à Gram négatif › Bâtonnets à Gram négatif anaérobies droits, incurvés et spiralés › Fusobacterium › Fusobacterium nucleatum › The Regional Specificity of Mucosa-Associated...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › The Regional Specificity of Mucosa-Associated...
questionsmedicales.fr › Environnement et santé publique › Environnement › Écosystème › Biodiversité › Biote › Microbiote › The Regional Specificity of Mucosa-Associated...
questionsmedicales.fr › Tissus › Membranes › Muqueuse › The Regional Specificity of Mucosa-Associated...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN ribosomique › ARN ribosomique 16S › The Regional Specificity of Mucosa-Associated...
questionsmedicales.fr › Phénomènes physiologiques cellulaires › Microenvironnement cellulaire › Microenvironnement tumoral › The Regional Specificity of Mucosa-Associated...