SALL1 Modulates CBX4 Stability, Nuclear Bodies, and Regulation of Target Genes.
CBX4
SALL1
SUMO
nuclear bodies
ubiquitin
Journal
Frontiers in cell and developmental biology
ISSN: 2296-634X
Titre abrégé: Front Cell Dev Biol
Pays: Switzerland
ID NLM: 101630250
Informations de publication
Date de publication:
2021
2021
Historique:
received:
27
05
2021
accepted:
30
08
2021
entrez:
8
10
2021
pubmed:
9
10
2021
medline:
9
10
2021
Statut:
epublish
Résumé
Development is orchestrated through a complex interplay of multiple transcription factors. The comprehension of this interplay will help us to understand developmental processes. Here we analyze the relationship between two key transcription factors: CBX4, a member of the Polycomb Repressive Complex 1 (PRC1), and SALL1, a member of the Spalt-like family with important roles in embryogenesis and limb development. Both proteins localize to nuclear bodies and are modified by the small ubiquitin-like modifier (SUMO). Our results show that CBX4 and SALL1 interact in the nucleoplasm and that increased SALL1 expression reduces ubiquitination of CBX4, enhancing its stability. This is accompanied by an increase in the number and size of CBX4-containing Polycomb bodies, and by a greater repression of CBX4 target genes. Thus, our findings uncover a new way of SALL1-mediated regulation of Polycomb bodies through modulation of CBX4 stability, with consequences in the regulation of its target genes, which could have an impact in cell differentiation and development.
Identifiants
pubmed: 34621739
doi: 10.3389/fcell.2021.715868
pmc: PMC8490708
doi:
Types de publication
Journal Article
Langues
eng
Pagination
715868Informations de copyright
Copyright © 2021 Giordano, Pirone, Muratore, Landaluze, Pérez, Lang, Garde-Lapido, Gonzalez-Lopez, Barroso-Gomila, Vertegaal, Aransay, Rodriguez, Rodriguez, Sutherland and Barrio.
Déclaration de conflit d'intérêts
VL is employed by the company Viralgen Vector Core. The remaining authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
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