Clinical and biochemical characteristics and outcomes of suspected COVID-19 hospitalized patients: RT-PCR swab positive and negative comparison.


Journal

Journal of infection and public health
ISSN: 1876-035X
Titre abrégé: J Infect Public Health
Pays: England
ID NLM: 101487384

Informations de publication

Date de publication:
Nov 2021
Historique:
received: 19 08 2021
revised: 16 09 2021
accepted: 19 09 2021
pubmed: 9 10 2021
medline: 11 11 2021
entrez: 8 10 2021
Statut: ppublish

Résumé

COVID-19 is diagnosed using RT-PCR assays of samples from nasal and oropharyngeal swabs. People with negative RT-PCR often presented with clinical manifestations of COVID-19. The data on such patients are lacking. The present study aims to characterize the patients who were suspected COVID-19 cases and tested negative in RT-PCR compared to patients who had been tested RT-PCR positive. This is a retrospective, observational study of adult suspected and confirmed patients of COVID-19 admitted to King Saud University Medical City, Riyadh, Saudi Arabia, from 1st March 2020 until 30th November 2020. Laboratory confirmation is done through nasal/pharyngeal swab specimens, tested positive in RT-PCR assay. Patients with initial negative RT-PCR test results were assessed again within 48-72 h to avoid false-negative results. Patient data were extracted from the electronic medical files of each included patient using a predesigned case report form. The study included 488 (80.93%) patients with RT-PCR swab results positive, and 115 (19.07%) patients who were negative. Respiratory rate and diastolic blood pressure were higher among the swab-positive cases. More number of swab-negative patients had comorbidities such as coronary heart disease, chronic kidney disease, and carcinoma. Fever, cough, and shortness of breath were reported higher among the swab-positive cases. ALT and AST, and LDH levels were found higher among RT-PCR-positive patients. Serum creatinine, blood urea nitrogen and troponin were more elevated in RT-PCR-negative patients. Antibiotics, anticoagulants, and corticosteroids were used more by swab-positive patients. Significantly higher number of RT-PCR-positive patients required proning, high-flow nasal cannula, non-invasive mechanical ventilation, and invasive mechanical ventilation. Acute cardiac ischemia and death were found to be similar among the patients. However, deaths occurred significantly earlier among the swab-positive cases when compared to the swab-negative group. Distinctive symptoms and markers of COVID-19 are more frequent among patients who had RT-PCR-positive results.

Sections du résumé

BACKGROUND BACKGROUND
COVID-19 is diagnosed using RT-PCR assays of samples from nasal and oropharyngeal swabs. People with negative RT-PCR often presented with clinical manifestations of COVID-19. The data on such patients are lacking. The present study aims to characterize the patients who were suspected COVID-19 cases and tested negative in RT-PCR compared to patients who had been tested RT-PCR positive.
METHODS METHODS
This is a retrospective, observational study of adult suspected and confirmed patients of COVID-19 admitted to King Saud University Medical City, Riyadh, Saudi Arabia, from 1st March 2020 until 30th November 2020. Laboratory confirmation is done through nasal/pharyngeal swab specimens, tested positive in RT-PCR assay. Patients with initial negative RT-PCR test results were assessed again within 48-72 h to avoid false-negative results. Patient data were extracted from the electronic medical files of each included patient using a predesigned case report form.
RESULTS RESULTS
The study included 488 (80.93%) patients with RT-PCR swab results positive, and 115 (19.07%) patients who were negative. Respiratory rate and diastolic blood pressure were higher among the swab-positive cases. More number of swab-negative patients had comorbidities such as coronary heart disease, chronic kidney disease, and carcinoma. Fever, cough, and shortness of breath were reported higher among the swab-positive cases. ALT and AST, and LDH levels were found higher among RT-PCR-positive patients. Serum creatinine, blood urea nitrogen and troponin were more elevated in RT-PCR-negative patients. Antibiotics, anticoagulants, and corticosteroids were used more by swab-positive patients. Significantly higher number of RT-PCR-positive patients required proning, high-flow nasal cannula, non-invasive mechanical ventilation, and invasive mechanical ventilation. Acute cardiac ischemia and death were found to be similar among the patients. However, deaths occurred significantly earlier among the swab-positive cases when compared to the swab-negative group.
CONCLUSION CONCLUSIONS
Distinctive symptoms and markers of COVID-19 are more frequent among patients who had RT-PCR-positive results.

Identifiants

pubmed: 34624717
pii: S1876-0341(21)00275-6
doi: 10.1016/j.jiph.2021.09.014
pmc: PMC8457915
pii:
doi:

Types de publication

Journal Article Observational Study

Langues

eng

Sous-ensembles de citation

IM

Pagination

1623-1629

Informations de copyright

Copyright © 2021. Published by Elsevier Ltd.

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Auteurs

Assim A Alfadda (AA)

Department of Internal Medicine, College of Medicine, and King Khalid University Hospital, King Saud University Medical City, King Saud University, Riyadh, Saudi Arabia; Strategic Center for Diabetes Research, College of Medicine, King Saud University, Riyadh, Saudi Arabia; Obesity Research Center, College of Medicine, King Saud University, Riyadh, Saudi Arabia. Electronic address: aalfadda@ksu.edu.sa.

Mohammad AlKhowaiter (M)

Department of Internal Medicine, College of Medicine, and King Khalid University Hospital, King Saud University Medical City, King Saud University, Riyadh, Saudi Arabia.

Naif Alotaibi (N)

Communicable Diseases Department, King Khalid University Hospital, King Saud University Medical City, King Saud University, Riyadh, Saudi Arabia.

Khalid Alayed (K)

Department of Internal Medicine, College of Medicine, and King Khalid University Hospital, King Saud University Medical City, King Saud University, Riyadh, Saudi Arabia.

Musa Alzahrani (M)

Department of Internal Medicine, College of Medicine, and King Khalid University Hospital, King Saud University Medical City, King Saud University, Riyadh, Saudi Arabia.

Khalifa Binkhamis (K)

Department of Pathology, College of Medicine, King Saud University, Riyadh, Saudi Arabia; King Saud University Medical City, King Saud University, Riyadh, Saudi Arabia.

Khalid Siddiqui (K)

Strategic Center for Diabetes Research, College of Medicine, King Saud University, Riyadh, Saudi Arabia.

Amira Youssef (A)

Strategic Center for Diabetes Research, College of Medicine, King Saud University, Riyadh, Saudi Arabia.

Haifa Altalhi (H)

Infection Control Department, King Khalid University Hospital, King Saud University Medical City, King Saud University, Riyadh, Saudi Arabia.

Ibrahim Almaghlouth (I)

Rheumatology Unit, Department of Internal Medicine, King Khalid University Hospital, King Saud University Medical City, King Saud University, Riyadh, Saudi Arabia.

Mohammed Alarifi (M)

Intensive Care Department, King Khalid University Hospital, King Saud University Medical City, King Saud University, Riyadh, Saudi Arabia.

Saleh Albanyan (S)

Department of Internal Medicine, College of Medicine, and King Khalid University Hospital, King Saud University Medical City, King Saud University, Riyadh, Saudi Arabia.

Mohammed Faraj Alosaimi (MF)

Immunology unit, Department of Pediatrics, College of Medicine, and King Khalid University Hospital, King Saud University Medical City, King Saud University, Riyadh, Saudi Arabia.

Rana Hasanato (R)

Department of Pathology, King Khalid University Hospital, King Saud University Medical City, King Saud University, Riyadh, Saudi Arabia.

Arthur Isnani (A)

Obesity Research Center, College of Medicine, King Saud University, Riyadh, Saudi Arabia.

Hafedh Dekhil (H)

Obesity Research Center, College of Medicine, King Saud University, Riyadh, Saudi Arabia.

Mohamed Rafiullah (M)

Strategic Center for Diabetes Research, College of Medicine, King Saud University, Riyadh, Saudi Arabia.

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Classifications MeSH