Cytotoxin antibody-based colourimetric sensor for field-level differential detection of elapid among big four snake venom.
Journal
PLoS neglected tropical diseases
ISSN: 1935-2735
Titre abrégé: PLoS Negl Trop Dis
Pays: United States
ID NLM: 101291488
Informations de publication
Date de publication:
10 2021
10 2021
Historique:
received:
24
07
2021
accepted:
27
09
2021
revised:
21
10
2021
pubmed:
12
10
2021
medline:
17
12
2021
entrez:
11
10
2021
Statut:
epublish
Résumé
Development of a rapid, on-site detection tool for snakebite is highly sought after, owing to its clinically and forensically relevant medicolegal significance. Polyvalent antivenom therapy in the management of such envenomation cases is finite due to its poor venom neutralization capabilities as well as diagnostic ramifications manifested as untoward immunological reactions. For precise molecular diagnosis of elapid venoms of the big four snakes, we have developed a lateral flow kit using a monoclonal antibody (AB1; IgG1 - κ chain; Kd: 31 nM) generated against recombinant cytotoxin-7 (rCTX-7; 7.7 kDa) protein of the elapid venom. The monoclonal antibody specifically detected the venoms of Naja naja (p < 0.0001) and Bungarus caeruleus (p<0.0001), without showing any immunoreactivity against the viperidae snakes in big four venomous snakes. The kit developed attained the limit of quantitation of 170 pg/μL and 2.1 ng/μL in spiked buffer samples and 28.7 ng/μL and 110 ng/μL in spiked serum samples for detection of N. naja and B. caeruleus venoms, respectively. This kit holds enormous potential in identification of elapid venom of the big four snakes for effective prognosis of an envenomation; as per the existing medical guidelines.
Identifiants
pubmed: 34634067
doi: 10.1371/journal.pntd.0009841
pii: PNTD-D-21-01114
pmc: PMC8530336
doi:
Substances chimiques
Antibodies, Monoclonal
0
Cytotoxins
0
Elapid Venoms
0
Immunotoxins
0
Snake Venoms
0
Types de publication
Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0009841Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
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