Biochemical Analysis of Leukocytes after In Vitro and In Vivo Activation with Bacterial and Fungal Pathogens Using Raman Spectroscopy.
Candida albicans
Klebsiella pneumoniae
PBMC
Raman microspectroscopy
Staphylococcus aureus
infection model
leukocytes
lymphocyte
monocyte
neutrophil
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
28 Sep 2021
28 Sep 2021
Historique:
received:
29
07
2021
revised:
14
09
2021
accepted:
23
09
2021
entrez:
13
10
2021
pubmed:
14
10
2021
medline:
27
10
2021
Statut:
epublish
Résumé
Biochemical information from activated leukocytes provide valuable diagnostic information. In this study, Raman spectroscopy was applied as a label-free analytical technique to characterize the activation pattern of leukocyte subpopulations in an in vitro infection model. Neutrophils, monocytes, and lymphocytes were isolated from healthy volunteers and stimulated with heat-inactivated clinical isolates of
Identifiants
pubmed: 34638822
pii: ijms221910481
doi: 10.3390/ijms221910481
pmc: PMC8508974
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : European Commission
ID : Raman4Clinics (BM1401)
Organisme : Seventh Framework Programme
ID : HemoSpec (CN 611682)
Organisme : Bundesministerium für Bildung und Forschung
ID : Centre for Sepsis Control and Care (FKZ 01EO1502).
Organisme : Leibniz-Gemeinschaft
ID : InfectoOptics (W8/2018)
Organisme : European Regional Development Fund
ID : ThIMEDOP (Thüringer Innovationszentrum für Medizintechnik-Lösungen, FKZ IZN 2018 0002)
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