Ionophore application for artificial oocyte activation and its potential effect on morphokinetics: a sibling oocyte study.
Adult
Birth Rate
Blastocyst
/ drug effects
Calcimycin
/ pharmacology
Embryo Transfer
/ methods
Embryonic Development
/ drug effects
Female
Fertilization in Vitro
/ methods
Humans
Ionophores
/ pharmacology
Live Birth
Male
Oocytes
/ drug effects
Pregnancy
Pregnancy Rate
Prospective Studies
Retrospective Studies
Sperm Injections, Intracytoplasmic
/ methods
Artificial oocyte activation
Calcimycin
Ionophore
Morphokinetics
Time-lapse imaging
Journal
Journal of assisted reproduction and genetics
ISSN: 1573-7330
Titre abrégé: J Assist Reprod Genet
Pays: Netherlands
ID NLM: 9206495
Informations de publication
Date de publication:
Dec 2021
Dec 2021
Historique:
received:
21
07
2021
accepted:
01
10
2021
pubmed:
14
10
2021
medline:
18
3
2022
entrez:
13
10
2021
Statut:
ppublish
Résumé
To evaluate whether ionophore application at the oocyte stage changes the morphokinetics of the associated embryos in cases of artificial oocyte activation. In a prospective sibling oocyte approach, 78 ICSI patients with suspected fertilization problems had half of their MII-oocytes treated with a ready-to-use ionophore (calcimycin) immediately following ICSI (study group). Untreated ICSI eggs served as the control group. Primary analyses focused on morphokinetic behavior and the presence of irregular cleavages. The rates of fertilization, utilization, pregnancy, and live birth rate were also evaluated. Ionophore-treated oocytes showed a significantly earlier formation of pronuclei (t2PNa) and a better synchronized third cell cycle (s3) (P < .05). The rate of irregular cleavage was unaffected (P > .05). Ionophore treatment significantly improved the overall rates of fertilization (P < .01) and blastocyst utilization (P < .05). Ionophore application does not negatively affect cleavage timing nor is it associated with irregular cleavage.
Identifiants
pubmed: 34642877
doi: 10.1007/s10815-021-02338-3
pii: 10.1007/s10815-021-02338-3
pmc: PMC8666403
doi:
Substances chimiques
Ionophores
0
Calcimycin
37H9VM9WZL
Types de publication
Journal Article
Observational Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
3125-3133Informations de copyright
© 2021. The Author(s).
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