Mycolactone Purification from M. ulcerans Cultures and HPLC-Based Approaches for Mycolactone Quantification in Biological Samples.

Chromatography, High Pressure Liquid Humans Macrolides Mycobacterium ulcerans Polyketide Synthases
Folch’s extraction LC-MS/MS Mycobacterium ulcerans Mycolactone TLC

Journal

Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Titre abrégé: Methods Mol Biol
Pays: United States
ID NLM: 9214969

Informations de publication

Date de publication:
2022
Historique:
entrez: 13 10 2021
pubmed: 14 10 2021
medline: 8 1 2022
Statut: ppublish

Résumé

Mycolactones are a family of polyketide synthase products made by the human pathogen Mycobacterium ulcerans that were recently identified as novel inhibitors of the host membrane translocation complex (Sec61). Here, we provide protocols for the purification of mycolactones from bacterial cultures, and for their quantitative assessment in biological samples.

Identifiants

DOI: 10.1007/978-1-0716-1779-3_13 PMID: 34643908
pubmed: 34643908
doi: 10.1007/978-1-0716-1779-3_13
doi:

Substances chimiques

Macrolides 0
mycolactone 0
Polyketide Synthases 79956-01-7

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

117-130

Informations de copyright

© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

Références

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Auteurs

Aline Rifflet (A)

Biology and Genetics of Bacterial Cell Wall, Institut Pasteur, Paris, France.
INSERM, Equipe Avenir, Paris, France.

Caroline Demangel (C)

Immunobiology of Infection Unit, Institut Pasteur, INSERM U1221, Paris, France.

Laure Guenin-Macé (L)

Immunobiology of Infection Unit, Institut Pasteur, INSERM U1221, Paris, France. laure.guenin-mace@pasteur.fr.

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Classifications MeSH

questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Chromatographie › Chromatographie en phase liquide › Chromatographie en phase liquide à haute performance › Mycolactone Purification from M. ulcerans...
questionsmedicales.fr › Eucaryotes › Animaux › Chordés › Vertébrés › Mammifères › Eutheria › Primates › Haplorhini › Catarrhini › Hominidae › Humains › Mycolactone Purification from M. ulcerans...
questionsmedicales.fr › Composés polycycliques › Composés macrocycliques › Polycétides › Macrolides › Mycolactone Purification from M. ulcerans...
questionsmedicales.fr › Bactéries › Bactéries à Gram positif › Bâtonnets à Gram positif › Bâtonnets asporogènes à Gram positif › Bâtonnets asporogènes réguliers à Gram positif › Mycobacteriaceae › Mycobacterium › Mycobactéries non tuberculeuses › Mycobacterium ulcerans › Mycolactone Purification from M. ulcerans...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Complexes multienzymatiques › Polyketide synthases › Mycolactone Purification from M. ulcerans...