RNF20 affects porcine adipocyte differentiation via regulation of mitotic clonal expansion.
C/EBPβ
RNF20
mitotic clonal expansion
porcine preadipocyte differentiation
Journal
Cell proliferation
ISSN: 1365-2184
Titre abrégé: Cell Prolif
Pays: England
ID NLM: 9105195
Informations de publication
Date de publication:
Dec 2021
Dec 2021
Historique:
revised:
30
08
2021
received:
04
08
2021
accepted:
07
09
2021
pubmed:
15
10
2021
medline:
24
12
2021
entrez:
14
10
2021
Statut:
ppublish
Résumé
RNF20 is recognized as a main E3 ligase for monoubiquitination of histone H2B at lysine 120 (H2Bub). The critical role of RNF20 and H2Bub in various molecular events, such as DNA replication, RNA transcription, and DNA damage response, has been widely investigated and documented. However, its role in porcine adipogenesis remains unknown. In this study, we aimed to clarify the effect of RNF20 on porcine preadipocyte differentiation. Backfat tissues from fat-type pigs (Bama and Meishan) and lean-type pigs (Yorkshire and Landrace) were collected to detect the expression level of RNF20. Preadipocytes were isolated from Bama piglets and induced to differentiation. Small interfering RNAs were applied to deplete RNF20. Oil Red O staining, quantitative real-time PCR, RNA-seq, Western blot analysis, and EdU assays were performed to study the regulatory mechanism of RNF20 during adipogenesis. We found that the expression levels of RNF20 and H2Bub were significantly higher in backfat tissues from fat-type pigs than in those from lean-type pigs. Consistently, the significantly induced expression of RNF20 and H2Bub was also observed in porcine differentiated adipocytes. In addition, knockdown of RNF20 greatly inhibited porcine adipogenesis, as evidenced by dramatically decreased lipid droplet formation and lower expression levels of adipogenic transcription masters in RNF20 knockdown cells. Mechanistically, the depletion of RNF20 decreases the cell proliferation and the level of p-C/EBPβ via the Ras-Raf-MEK1/2-ERK1/2 cascade pathway at the mitotic clonal expansion phase and therefore suppresses cell differentiation. Our results demonstrate that RNF20 is required for porcine preadipocyte differentiation.
Identifiants
pubmed: 34647359
doi: 10.1111/cpr.13131
pmc: PMC8666272
doi:
Substances chimiques
Histones
0
Ubiquitin-Protein Ligases
EC 2.3.2.27
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e13131Subventions
Organisme : Fundamental Research Funds for Central Nonprofit Scientific Institution
ID : 2021-YWF-ZYSQ-03
Organisme : National Natural Science Foundation of China
ID : 31925036
Organisme : National Natural Science Foundation of China
ID : 32025034
Organisme : National Key R&D Program of China
ID : 2020YFA0509503
Informations de copyright
© 2021 The Authors. Cell Proliferation Published by John Wiley & Sons Ltd.
Références
J Biosci Bioeng. 2014 May;117(5):624-31
pubmed: 24331979
Expert Opin Ther Targets. 2012 Jan;16(1):103-19
pubmed: 22239440
Acta Biochim Biophys Sin (Shanghai). 2014 Feb;46(2):136-40
pubmed: 24374663
Mol Cell Biol. 2012 Mar;32(6):1044-55
pubmed: 22252316
J Mol Cell Biol. 2017 Oct 1;9(5):364-375
pubmed: 28486585
J Biol Chem. 2015 Jan 9;290(2):755-61
pubmed: 25451943
Nat Chem Biol. 2011 Feb;7(2):113-9
pubmed: 21196936
J Mol Cell Biol. 2020 Feb 20;12(2):113-124
pubmed: 31152661
Mol Cell. 2003 Jan;11(1):261-6
pubmed: 12535538
Cell Prolif. 2021 Dec;54(12):e13131
pubmed: 34647359
Genes Brain Behav. 2006;5 Suppl 2:61-72
pubmed: 16681801
Biochim Biophys Acta. 2014 Aug;1839(8):694-701
pubmed: 24412854
Protein Cell. 2021 Jun;12(6):475-492
pubmed: 32797353
Mol Cell. 2012 Jan 13;45(1):132-9
pubmed: 22244335
Animals (Basel). 2020 May 20;10(5):
pubmed: 32443664
Diabetes. 2020 Jan;69(1):20-34
pubmed: 31604693
Hepatology. 2014 Sep;60(3):844-57
pubmed: 24425205
Mol Cell. 2013 Mar 28;49(6):1019-20
pubmed: 23541037
Mol Cell. 2012 Jun 8;46(5):705-13
pubmed: 22681891
Annu Rev Biochem. 1995;64:345-73
pubmed: 7574486
Cell Death Differ. 2018 Feb;25(2):294-306
pubmed: 28984873
Genome Res. 2012 Jun;22(6):1026-35
pubmed: 22421545
Nat Rev Mol Cell Biol. 2006 Dec;7(12):885-96
pubmed: 17139329
Annu Rev Nutr. 1994;14:99-129
pubmed: 7946535
Radiat Res. 2010 Nov;174(5):558-65
pubmed: 20738173
Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):850-5
pubmed: 12525691
Physiol Rev. 1998 Jul;78(3):783-809
pubmed: 9674695
Stem Cells. 2009 Sep;27(9):2092-102
pubmed: 19544411
EMBO Rep. 2009 Aug;10(8):894-900
pubmed: 19575011
Mol Cell. 2012 Dec 14;48(5):734-46
pubmed: 23103252
Adv Cancer Res. 1998;74:49-139
pubmed: 9561267
Proc Natl Acad Sci U S A. 2003 Jan 7;100(1):44-9
pubmed: 12502791
Mol Cell. 2011 Mar 4;41(5):515-28
pubmed: 21362548
Animals (Basel). 2020 Mar 03;10(3):
pubmed: 32138208
Nucleic Acids Res. 2016 Nov 16;44(20):9681-9697
pubmed: 27431324
Genes Dev. 1999 Sep 1;13(17):2231-41
pubmed: 10485846
Biochem J. 2002 Jun 15;364(Pt 3):739-46
pubmed: 12049638
Mol Cell. 2003 Jan;11(1):267-74
pubmed: 12535539