Replication and single-cycle delivery of SARS-CoV-2 replicons.
Animals
Antibodies, Neutralizing
/ immunology
Antibodies, Viral
/ immunology
Antiviral Agents
/ pharmacology
Cell Line
Humans
Interferons
/ pharmacology
Microbial Sensitivity Tests
Mutation
Plasmids
RNA, Viral
/ genetics
Replicon
/ genetics
Reverse Genetics
SARS-CoV-2
/ drug effects
Saccharomyces cerevisiae
/ genetics
Spike Glycoprotein, Coronavirus
/ genetics
Viral Nonstructural Proteins
/ genetics
Viral Pseudotyping
Virion
/ genetics
Virus Replication
Journal
Science (New York, N.Y.)
ISSN: 1095-9203
Titre abrégé: Science
Pays: United States
ID NLM: 0404511
Informations de publication
Date de publication:
26 Nov 2021
26 Nov 2021
Historique:
pubmed:
15
10
2021
medline:
15
12
2021
entrez:
14
10
2021
Statut:
ppublish
Résumé
Molecular virology tools are critical for basic studies of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) and for developing new therapeutics. Experimental systems that do not rely on viruses capable of spread are needed for potential use in lower-containment settings. In this work, we use a yeast-based reverse genetics system to develop spike-deleted SARS-CoV-2 self-replicating RNAs. These noninfectious self-replicating RNAs, or replicons, can be trans-complemented with viral glycoproteins to generate replicon delivery particles for single-cycle delivery into a range of cell types. This SARS-CoV-2 replicon system represents a convenient and versatile platform for antiviral drug screening, neutralization assays, host factor validation, and viral variant characterization.
Identifiants
pubmed: 34648371
doi: 10.1126/science.abj8430
pmc: PMC9007107
mid: NIHMS1788959
doi:
Substances chimiques
Antibodies, Neutralizing
0
Antibodies, Viral
0
Antiviral Agents
0
NSP1 protein, SARS-CoV-2
0
RNA, Viral
0
Spike Glycoprotein, Coronavirus
0
Viral Nonstructural Proteins
0
spike protein, SARS-CoV-2
0
Interferons
9008-11-1
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
1099-1106Subventions
Organisme : NIAID NIH HHS
ID : R01 AI150275
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI116943
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA016087
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI091707
Pays : United States
Organisme : NCI NIH HHS
ID : U01 CA213359
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA190261
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI124690
Pays : United States
Organisme : NIAID NIH HHS
ID : P01 AI138938
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA008748
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA213448
Pays : United States
Organisme : NIAID NIH HHS
ID : U19 AI111825
Pays : United States
Organisme : NIAID NIH HHS
ID : R03 AI141855
Pays : United States
Organisme : NIGMS NIH HHS
ID : P41 GM109824
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI143295
Pays : United States
Organisme : NIAID NIH HHS
ID : R21 AI142010
Pays : United States
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