Changes in porcine nutrient transport physiology in response to Ascaris suum infection.
Alanine transport
Ascariasis
Ascariosis
Dipeptide transport
Electrophysiology
Glucose transport
Small intestine
Ussing chamber
Journal
Parasites & vectors
ISSN: 1756-3305
Titre abrégé: Parasit Vectors
Pays: England
ID NLM: 101462774
Informations de publication
Date de publication:
14 Oct 2021
14 Oct 2021
Historique:
received:
24
06
2021
accepted:
20
09
2021
entrez:
15
10
2021
pubmed:
16
10
2021
medline:
8
1
2022
Statut:
epublish
Résumé
The roundworm Ascaris suum is one of the parasites with the greatest economic impact on pig farming. In this context, lower weight gain is hypothesized to be due to decreased nutrient absorption. This study aims at characterizing the effects of A. suum infection on intestinal nutrient transport processes and potential molecular mechanisms. Three groups of six piglets each were infected orally (10,000 embryonated A. suum eggs) in a single dose ("single infection"). Another three groups were infected orally (1000 embryonated eggs) for 10 consecutive days ("trickle infection"). Animals were necropsied 21, 35 and 49 days post-infection (dpi). Three groups served as respective controls. The Ussing chamber technique was applied for the functional characterization of small intestinal tissues [short-circuit currents (I Peptide- and alanine-induced changes in I In contrast to the current hypothesis, these results indicate that the nutrient deprivation induced by A. suum cannot be explained by transcriptional or expression changes alone and requires further studies.
Sections du résumé
BACKGROUND
BACKGROUND
The roundworm Ascaris suum is one of the parasites with the greatest economic impact on pig farming. In this context, lower weight gain is hypothesized to be due to decreased nutrient absorption. This study aims at characterizing the effects of A. suum infection on intestinal nutrient transport processes and potential molecular mechanisms.
METHODS
METHODS
Three groups of six piglets each were infected orally (10,000 embryonated A. suum eggs) in a single dose ("single infection"). Another three groups were infected orally (1000 embryonated eggs) for 10 consecutive days ("trickle infection"). Animals were necropsied 21, 35 and 49 days post-infection (dpi). Three groups served as respective controls. The Ussing chamber technique was applied for the functional characterization of small intestinal tissues [short-circuit currents (I
RESULTS
RESULTS
Peptide- and alanine-induced changes in I
CONCLUSIONS
CONCLUSIONS
In contrast to the current hypothesis, these results indicate that the nutrient deprivation induced by A. suum cannot be explained by transcriptional or expression changes alone and requires further studies.
Identifiants
pubmed: 34649607
doi: 10.1186/s13071-021-05029-1
pii: 10.1186/s13071-021-05029-1
pmc: PMC8515719
doi:
Substances chimiques
Cytokines
0
Peptides
0
Glucose
IY9XDZ35W2
Alanine
OF5P57N2ZX
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
533Subventions
Organisme : Deutsche Forschungsgemeinschaft
ID : BR 780/23-1
Organisme : Deutsche Forschungsgemeinschaft
ID : STR 1171/14-1
Informations de copyright
© 2021. The Author(s).
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