Notch Intracellular Domain Plasmid Delivery via Poly(Lactic-Co-Glycolic Acid) Nanoparticles to Upregulate Notch Pathway Molecules.
Notch signaling
PLGA
gene delivery
nanoparticles
non-viral transfection
Journal
Frontiers in cardiovascular medicine
ISSN: 2297-055X
Titre abrégé: Front Cardiovasc Med
Pays: Switzerland
ID NLM: 101653388
Informations de publication
Date de publication:
2021
2021
Historique:
received:
10
05
2021
accepted:
20
08
2021
entrez:
15
10
2021
pubmed:
16
10
2021
medline:
16
10
2021
Statut:
epublish
Résumé
Notch signaling is a highly conserved signaling system that is required for embryonic development and regeneration of organs. When the signal is lost, maldevelopment occurs and leads to a lethal state. Delivering exogenous genetic materials encoding Notch into cells can reestablish downstream signaling and rescue cellular functions. In this study, we utilized the negatively charged and FDA approved polymer poly(lactic-co-glycolic acid) to encapsulate Notch Intracellular Domain-containing plasmid in nanoparticles. We show that primary human umbilical vein endothelial cells (HUVECs) readily uptake the nanoparticles with and without specific antibody targets. We demonstrated that our nanoparticles are non-toxic, stable over time, and compatible with blood. We further demonstrated that HUVECs could be successfully transfected with these nanoparticles in static and dynamic environments. Lastly, we elucidated that these nanoparticles could upregulate the downstream genes of Notch signaling, indicating that the payload was viable and successfully altered the genetic downstream effects.
Identifiants
pubmed: 34651022
doi: 10.3389/fcvm.2021.707897
pmc: PMC8507495
doi:
Types de publication
Journal Article
Langues
eng
Pagination
707897Commentaires et corrections
Type : ErratumIn
Informations de copyright
Copyright © 2021 Messerschmidt, Chintapula, Kuriakose, Laboy, Truong, Kydd, Jaworski, Pan, Sadek, Nguyen and Lee.
Déclaration de conflit d'intérêts
The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.
Références
Oncotarget. 2017 May 22;8(40):67189-67202
pubmed: 28978026
Sci Rep. 2017 Oct 16;7(1):13249
pubmed: 29038584
FEBS J. 2016 Dec;283(23):4223-4237
pubmed: 27260948
Polymers (Basel). 2011 Sep 1;3(3):1377-1397
pubmed: 22577513
Cell. 2009 Apr 17;137(2):216-33
pubmed: 19379690
Int J Pharm. 2019 Apr 5;560:306-314
pubmed: 30797073
Nano Lett. 2017 Mar 8;17(3):1326-1335
pubmed: 28273716
J Am Coll Cardiol. 2009 Feb 24;53(8):709-17
pubmed: 19232905
Dev Neurobiol. 2020 Mar;80(3-4):85-97
pubmed: 31587468
Iran J Pharm Res. 2019 Winter;18(1):125-141
pubmed: 31089350
ACS Omega. 2020 Jun 09;5(24):14730-14740
pubmed: 32596610
J Pharm Pharm Sci. 2004 Jun 30;7(2):205-14
pubmed: 15367377
J Microencapsul. 2020 May;37(3):283-295
pubmed: 32079451
Dev Cell. 2012 Feb 14;22(2):244-54
pubmed: 22340493
Drug Deliv Transl Res. 2020 Jun;10(3):582-593
pubmed: 31919746
Adv Drug Deliv Rev. 2003 Feb 24;55(3):329-47
pubmed: 12628320
Top Curr Chem (Cham). 2017 Apr;375(2):35
pubmed: 28290155
Pharmaceutics. 2020 Jul 03;12(7):
pubmed: 32635142
Science. 2015 Jan 23;347(6220):1260419
pubmed: 25613900
J Microencapsul. 2015;32(7):699-704
pubmed: 26004370
Proc Natl Acad Sci U S A. 2017 Apr 25;114(17):4376-4381
pubmed: 28396439
Nanoscale Res Lett. 2011 Jul 12;6:447
pubmed: 21749688
J Am Chem Soc. 2011 Apr 13;133(14):5508-15
pubmed: 21428437
J Biomed Mater Res A. 2011 Dec 15;99(4):607-17
pubmed: 21953904
Pharmaceutics. 2020 Nov 29;12(12):
pubmed: 33260446
Mol Ther. 2016 Feb;24(1):66-75
pubmed: 26471463
Biomacromolecules. 2017 Dec 11;18(12):4307-4315
pubmed: 29141136
J Mol Cell Cardiol. 2008 Oct;45(4):495-504
pubmed: 18410944
Bioelectricity. 2020 Jun 1;2(2):150-157
pubmed: 32856017
Math Biosci Eng. 2021 Mar 11;18(3):2401-2424
pubmed: 33892552
Biochem Biophys Res Commun. 2003 May 2;304(2):399-404
pubmed: 12711329
Nanomedicine. 2018 Oct;14(7):2095-2102
pubmed: 29969727
Antioxid Redox Signal. 2018 May 1;28(13):1209-1223
pubmed: 29037123
Iran J Pharm Res. 2019 Winter;18(1):156-167
pubmed: 31089352
AAPS PharmSciTech. 2020 Jan 29;21(3):85
pubmed: 31997020
Polymers (Basel). 2019 Feb 12;11(2):
pubmed: 30960288
Development. 2018 Sep 21;145(18):
pubmed: 30126902
BMC Evol Biol. 2009 Oct 13;9:249
pubmed: 19825158
Drug Dev Ind Pharm. 2020 Apr;46(4):682-695
pubmed: 32281428
Int J Pharm. 2017 May 30;524(1-2):77-90
pubmed: 28359811
Mucosal Immunol. 2018 Jul;11(4):1079-1091
pubmed: 29467447
J Control Release. 2007 Jul 16;120(1-2):18-26
pubmed: 17509712
Am J Physiol Heart Circ Physiol. 2010 Jun;298(6):H1959-65
pubmed: 20228260
ACS Nano. 2014 May 27;8(5):5141-53
pubmed: 24766032
Cold Spring Harb Symp Quant Biol. 2009;74:81-90
pubmed: 19903747
Mech Dev. 2006 Aug;123(8):626-40
pubmed: 16843648
J Microencapsul. 2018 Mar;35(2):204-217
pubmed: 29542378
Proc Natl Acad Sci U S A. 2003 Sep 30;100 Suppl 1:11889-95
pubmed: 12909711
Bioconjug Chem. 2019 Feb 20;30(2):338-349
pubmed: 30383373
Artif Cells Nanomed Biotechnol. 2018 Dec;46(8):1781-1791
pubmed: 29081256
Differentiation. 2012 Jul;84(1):4-16
pubmed: 22704690
Eur J Pharm Biopharm. 2011 Jan;77(1):26-35
pubmed: 21093589
Adv Drug Deliv Rev. 2007 Mar 30;59(2-3):134-40
pubmed: 17451840
Stem Cells. 2007 Jan;25(1):203-10
pubmed: 16973835
Biomaterials. 2019 Mar;195:23-37
pubmed: 30610991
Nat Med. 2013 Feb;19(2):193-201
pubmed: 23314057
Nucleic Acids Res. 2001 Sep 15;29(18):3882-91
pubmed: 11557821
JCI Insight. 2018 Jul 12;3(13):
pubmed: 29997298
Front Immunol. 2021 Feb 05;11:609324
pubmed: 33613531
J Phys Chem B. 2018 Jul 19;122(28):7080-7090
pubmed: 29927603
Crit Rev Ther Drug Carrier Syst. 2016;33(2):159-93
pubmed: 27651101
Nat Rev Genet. 2008 Jan;9(1):49-61
pubmed: 18071321
J Clin Invest. 2016 May 2;126(5):1679-90
pubmed: 27018592
Nat Biotechnol. 2013 Oct;31(10):898-907
pubmed: 24013197
Hum Vaccin Immunother. 2016 Mar 3;12(3):806-28
pubmed: 26513024
Eur J Pharm Biopharm. 2020 May;150:131-142
pubmed: 32151727
Tissue Cell. 1974;6(3):447-62
pubmed: 4432234
Int J Nanomedicine. 2013;8:3071-90
pubmed: 23990720
J Control Release. 2007 May 14;119(1):77-85
pubmed: 17349712
Biopolymers. 2007 Apr 5-15;85(5-6):379-91
pubmed: 17266128
Biomacromolecules. 2011 Apr 11;12(4):1006-14
pubmed: 21323308
Nanomedicine. 2019 Apr;17:297-307
pubmed: 30794963
Adv Pharm Bull. 2018 Mar;8(1):77-84
pubmed: 29670842
Pharmaceut Med. 2011 Oct 1;25(5):293-306
pubmed: 22200988
Am J Med Genet C Semin Med Genet. 2013 Aug;163C(3):157-68
pubmed: 23720419
J Nanobiotechnology. 2018 Jan 29;16(1):8
pubmed: 29378591
Am J Physiol. 1991 Oct;261(4 Suppl):60-5
pubmed: 1928455
Acta Biomater. 2014 Jun;10(6):2643-52
pubmed: 24512977
Blood. 2012 Apr 12;119(15):3585-94
pubmed: 22368273
Nat Commun. 2016 Jun 30;7:12088
pubmed: 27357444
Eur J Pharm Biopharm. 2018 Apr;125:38-50
pubmed: 29325770
J Control Release. 2006 Aug 10;114(1):100-9
pubmed: 16831482
Int J Oncol. 2008 Mar;32(3):643-51
pubmed: 18292942