Inhibition of Severe Acute Respiratory Syndrome Coronavirus 2 Replication by Hypertonic Saline Solution in Lung and Kidney Epithelial Cells.
Journal
ACS pharmacology & translational science
ISSN: 2575-9108
Titre abrégé: ACS Pharmacol Transl Sci
Pays: United States
ID NLM: 101721411
Informations de publication
Date de publication:
08 Oct 2021
08 Oct 2021
Historique:
received:
15
03
2021
entrez:
15
10
2021
pubmed:
16
10
2021
medline:
16
10
2021
Statut:
epublish
Résumé
An unprecedented global health crisis has been caused by a new virus called severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We performed experiments to test if a hypertonic saline solution was capable of inhibiting virus replication. Our data show that 1.2% NaCl inhibited virus replication by 90%, achieving 100% of inhibition at 1.5% in the nonhuman primate kidney cell line Vero, and 1.1% of NaCl was sufficient to inhibit the virus replication by 88% in human epithelial lung cell line Calu-3. Furthermore, our results indicate that the inhibition is due to an intracellular mechanism and not to the dissociation of the spike SARS-CoV-2 protein and its human receptor. NaCl depolarizes the plasma membrane causing a low energy state (high ADP/ATP concentration ratio) without impairing mitochondrial function, supposedly associated with the inhibition of the SARS-CoV-2 life cycle. Membrane depolarization and intracellular energy deprivation are possible mechanisms by which the hypertonic saline solution efficiently prevents virus replication
Identifiants
pubmed: 34651104
doi: 10.1021/acsptsci.1c00080
pmc: PMC8442612
doi:
Types de publication
Journal Article
Langues
eng
Pagination
1514-1527Informations de copyright
© 2021 American Chemical Society.
Déclaration de conflit d'intérêts
The authors declare no competing financial interest.
Références
Eur J Pharmacol. 2021 Feb 5;892:173782
pubmed: 33279521
Cochrane Database Syst Rev. 2017 Dec 21;12:CD006458
pubmed: 29265171
Annu Rev Virol. 2016 Sep 29;3(1):237-261
pubmed: 27578435
Sci Transl Med. 2015 Nov 4;7(312):312ra177
pubmed: 26537257
Ann Transl Med. 2016 Oct;4(Suppl 1):S11
pubmed: 27867979
PLoS One. 2015 Jun 04;10(6):e0130107
pubmed: 26042603
J Infect. 2020 Jun;80(6):607-613
pubmed: 32283152
Drug Discov Today. 2007 Sep;12(17-18):777-85
pubmed: 17826691
J Appl Physiol (1985). 2002 Nov;93(5):1852-9
pubmed: 12381774
Mol Psychiatry. 2021 Jun;26(6):2633-2650
pubmed: 32350390
J Virol. 2004 Jun;78(11):5642-50
pubmed: 15140961
Science. 2020 Mar 13;367(6483):1260-1263
pubmed: 32075877
J Virol. 2009 Apr;83(8):3778-87
pubmed: 19193784
Sci Rep. 2019 Jan 31;9(1):1015
pubmed: 30705369
Annu Rev Pharmacol Toxicol. 2020 Jan 6;60:175-194
pubmed: 31561739
Front Med Technol. 2021 Jan 13;2:614652
pubmed: 35047894
Biochim Biophys Acta. 2015 Feb;1847(2):171-181
pubmed: 25449966
Euro Surveill. 2020 Jan;25(3):
pubmed: 31992387
J Biol Chem. 2007 Mar 2;282(9):6153-60
pubmed: 17199078
N Engl J Med. 2020 Feb 20;382(8):727-733
pubmed: 31978945
Proc Natl Acad Sci U S A. 2000 Aug 29;97(18):10282-7
pubmed: 10920189
Cell Res. 2020 Mar;30(3):269-271
pubmed: 32020029
Am Fam Physician. 2009 Nov 15;80(10):1117-9
pubmed: 19904896
J Physiol. 2009 Jul 1;587(Pt 13):3055
pubmed: 19567739
Viruses. 2020 Jan 03;12(1):
pubmed: 31947719
Int J Immunopathol Pharmacol. 2020 Jan-Dec;34:2058738420941757
pubmed: 32799596
Nat Genet. 1996 Mar;12(3):325-8
pubmed: 8589728
J Pharmacol Sci. 2014;125(3):312-9
pubmed: 24989838
Proc Natl Acad Sci U S A. 2004 Nov 2;101(44):15748-53
pubmed: 15496474
N Engl J Med. 2003 May 15;348(20):1967-76
pubmed: 12690091
Antiviral Res. 2008 Nov;80(2):124-34
pubmed: 18585796
N Engl J Med. 2021 May 13;384(19):1866-1868
pubmed: 33761203
Am J Physiol Cell Physiol. 2005 Feb;288(2):C403-15
pubmed: 15456696
Nat Microbiol. 2020 Apr;5(4):562-569
pubmed: 32094589
Cytometry. 1994 Sep 1;17(1):59-65
pubmed: 7528124
Acta Physiol Scand. 2000 Oct;170(2):99-109
pubmed: 11114947
Clin Microbiol Infect. 2011 Dec;17(12):1829-33
pubmed: 20636429
Mem Inst Oswaldo Cruz. 2020 Oct 23;115:e200342
pubmed: 33111751
Sci Signal. 2011 Feb 01;4(158):ra7
pubmed: 21285412
Cell Res. 2008 Feb;18(2):290-301
pubmed: 18227861
N Engl J Med. 2020 Mar 19;382(12):1177-1179
pubmed: 32074444
Pflugers Arch. 2015 Mar;467(3):531-42
pubmed: 25482671
J Glob Health. 2020 Jun;10(1):010332
pubmed: 32395245
Respir Res. 2020 Jul 8;21(1):175
pubmed: 32641036
J Biol Chem. 2009 Mar 13;284(11):7294-306
pubmed: 19131335
J Gen Virol. 2017 Mar;98(3):345-351
pubmed: 28113044
Am J Physiol Lung Cell Mol Physiol. 2009 Mar;296(3):L372-83
pubmed: 19112100
Signal Transduct Target Ther. 2020 Dec 4;5(1):283
pubmed: 33277466
Nature. 2020 Sep;585(7826):588-590
pubmed: 32698190
JAMA Otolaryngol Head Neck Surg. 2020 Sep 1;146(9):787-788
pubmed: 32722777
Nat Rev Dis Primers. 2016 Oct 20;2:16080
pubmed: 27775730
Cell. 2020 Apr 16;181(2):271-280.e8
pubmed: 32142651
J Phys Chem Lett. 2020 Dec 17;11(24):10446-10453
pubmed: 33269932
N Engl J Med. 2006 Jan 19;354(3):229-40
pubmed: 16421364
Annu Rev Physiol. 1999;61:627-61
pubmed: 10099704
J Virol. 2003 Aug;77(16):8801-11
pubmed: 12885899
Lancet. 2021 Apr 3;397(10281):1263-1264
pubmed: 33765410
Nature. 2020 May;581(7807):215-220
pubmed: 32225176
ACS Cent Sci. 2020 Nov 25;6(11):2046-2052
pubmed: 33269329
J Biol Chem. 2018 Jan 19;293(3):1030-1039
pubmed: 29203528
Sci Rep. 2018 Sep 11;8(1):13630
pubmed: 30206371
Lancet Microbe. 2020 May;1(1):e14-e23
pubmed: 32835326
Virol Sin. 2020 Jun;35(3):311-320
pubmed: 32602046