Phospholipase C-zeta levels are not correlated with fertilisation rates in infertile couples.
flow cytometry
human spermatozoa
male infertility
phospholipase C-zeta
total fertilisation failure
Journal
Andrologia
ISSN: 1439-0272
Titre abrégé: Andrologia
Pays: Germany
ID NLM: 0423506
Informations de publication
Date de publication:
Feb 2022
Feb 2022
Historique:
revised:
20
09
2021
received:
28
05
2021
accepted:
22
09
2021
pubmed:
16
10
2021
medline:
12
1
2022
entrez:
15
10
2021
Statut:
ppublish
Résumé
In mammals, 'oocyte activation' is triggered by certain proteins, one of which is phospholipase C-zeta. Recent evidence suggests that low expression of phospholipase C-zeta might be associated with male infertility, while a limited number of studies claimed the opposite. This study was designed to test whether quantity of phospholipase C-zeta and in vitro fertilisation rates are correlated or not, assessed by flow cytometry. Semen samples from 43 infertile couples were analysed for the percentage and mean fluorescent intensity (MFI) of phospholipase C-zeta protein. Results were confirmed by immunofluorescent labelling. Patients with a fertilisation rate of 40% or lower were involved in the low fertilisation group, while the high fertilization group consisted of patients with a fertilisation rate of 60% and higher. Quantitative analyses by flow cytometry showed no significant difference among the low fertilisation and high fertilisation groups when phospholipase C-zeta ratio or MFI was considered. No correlation was found between pregnancy rates and phospholipase C-zeta quantity. None of the total fertilisation failure cases were lack of phospholipase C-zeta. In fact, fertilisation was possible even when phospholipase C-zeta levels were very low. Thus, we concluded that phospholipase C-zeta quantity cannot be considered as a diagnostic tool for male infertility.
Substances chimiques
Type C Phospholipases
EC 3.1.4.-
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e14269Subventions
Organisme : TUBİTAK
ID : 213S019
Informations de copyright
© 2021 Wiley-VCH GmbH.
Références
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