Parasitism by endoparasitoid wasps alters the internal but not the external microbiome in host caterpillars.
Cotesia glomerata
Microbial community
Parasitism
Pieris brassicae
Trophic interactions
Wolbachia
Journal
Animal microbiome
ISSN: 2524-4671
Titre abrégé: Anim Microbiome
Pays: England
ID NLM: 101759457
Informations de publication
Date de publication:
15 Oct 2021
15 Oct 2021
Historique:
received:
18
02
2021
accepted:
01
10
2021
entrez:
16
10
2021
pubmed:
17
10
2021
medline:
17
10
2021
Statut:
epublish
Résumé
The microbiome of many insects consists of a diverse community of microorganisms that can play critical roles in the functioning and overall health of their hosts. Although the microbial communities of insects have been studied thoroughly over the past decade, little is still known about how biotic interactions affect the microbial community structure in and on the bodies of insects. In insects that are attacked by parasites or parasitoids, it can be expected that the microbiome of the host insect is affected by the presence of these parasitic organisms that develop in close association with their host. In this study, we used high-throughput amplicon sequencing targeting both bacteria and fungi to test the hypothesis that parasitism by the endoparasitoid Cotesia glomerata affected the microbiome of its host Pieris brassicae. Healthy and parasitized caterpillars were collected from both natural populations and a laboratory culture. Significant differences in bacterial community structure were found between field-collected caterpillars and laboratory-reared caterpillars, and between the external and the internal microbiome of the caterpillars. Parasitism significantly altered the internal microbiome of caterpillars, but not the external microbiome. The internal microbiome of all parasitized caterpillars and of the parasitoid larvae in the caterpillar hosts was dominated by a Wolbachia strain, which was completely absent in healthy caterpillars, suggesting that the strain was transferred to the caterpillars during oviposition by the parasitoids. We conclude that biotic interactions such as parasitism have pronounced effects on the microbiome of an insect host and possibly affect interactions with higher-order insects.
Sections du résumé
BACKGROUND
BACKGROUND
The microbiome of many insects consists of a diverse community of microorganisms that can play critical roles in the functioning and overall health of their hosts. Although the microbial communities of insects have been studied thoroughly over the past decade, little is still known about how biotic interactions affect the microbial community structure in and on the bodies of insects. In insects that are attacked by parasites or parasitoids, it can be expected that the microbiome of the host insect is affected by the presence of these parasitic organisms that develop in close association with their host. In this study, we used high-throughput amplicon sequencing targeting both bacteria and fungi to test the hypothesis that parasitism by the endoparasitoid Cotesia glomerata affected the microbiome of its host Pieris brassicae. Healthy and parasitized caterpillars were collected from both natural populations and a laboratory culture.
RESULTS
RESULTS
Significant differences in bacterial community structure were found between field-collected caterpillars and laboratory-reared caterpillars, and between the external and the internal microbiome of the caterpillars. Parasitism significantly altered the internal microbiome of caterpillars, but not the external microbiome. The internal microbiome of all parasitized caterpillars and of the parasitoid larvae in the caterpillar hosts was dominated by a Wolbachia strain, which was completely absent in healthy caterpillars, suggesting that the strain was transferred to the caterpillars during oviposition by the parasitoids.
CONCLUSION
CONCLUSIONS
We conclude that biotic interactions such as parasitism have pronounced effects on the microbiome of an insect host and possibly affect interactions with higher-order insects.
Identifiants
pubmed: 34654483
doi: 10.1186/s42523-021-00135-y
pii: 10.1186/s42523-021-00135-y
pmc: PMC8520287
doi:
Types de publication
Journal Article
Langues
eng
Pagination
73Subventions
Organisme : Fonds Wetenschappelijk Onderzoek
ID : FWO
Organisme : Fonds Wetenschappelijk Onderzoek
ID : G.0961.19N
Organisme : Nederlandse Organisatie voor Wetenschappelijk Onderzoek
ID : ALWOP.343
Organisme : Nederlandse Organisatie voor Wetenschappelijk Onderzoek
ID : ALWOP.368
Informations de copyright
© 2021. The Author(s).
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