An experimental study on long term outcomes after magnetic esophageal compression anastomosis in piglets.


Journal

Journal of pediatric surgery
ISSN: 1531-5037
Titre abrégé: J Pediatr Surg
Pays: United States
ID NLM: 0052631

Informations de publication

Date de publication:
Jan 2022
Historique:
received: 23 08 2021
accepted: 08 09 2021
pubmed: 18 10 2021
medline: 2 2 2022
entrez: 17 10 2021
Statut: ppublish

Résumé

Previous studies have shown that a patent, watertight esophageal anastomosis can be accomplished safely using specially-shaped magnets in piglets. However, it is unclear whether such a magnetic esophageal compression anastomosis (MECA) remains patent in the long-term. The purpose of this study was to evaluate the long-term outcome of MECA in an experimental pig model over an observation period of 2 months. Ten piglets underwent creation of an MECA with custom-made 8 mm magnets and a U-shaped esophageal bypass loop to allow peroral nutrition at eight weeks of life. Two weeks later, the bypass loop was closed surgically, requiring the pigs to swallow via the newly created magnetic compression anastomosis. The pigs were fed soft chow for 2 months. They were monitored for weight gain and signs of dysphagia. At the endpoint of two months, esophagoscopy and contrast esophagography was performed. After removal of the esophagus, the tissues were macroscopiocally and histologically assessed. Six piglets survived until the endpoint. In two pigs, closure of the bypass loop failed, these demonstrated mean weight gain of 792 gs/day [95% Confidence interval 575 to 1009 gs/day]. Weight gain in four pigs that exclusively fed via the magnetic anastomosis averaged 577 gs/day [95% confidence interval 434 to 719 gs/day (p = 0.18)]. There were no signs of dysphagia. All magnets passed with the stool within 16 days. After 2 months, a well-formed magnetic compression anastomosis was visible and easily negotiated with a 6.5 mm endoscope. Esophogram and macroscopic findings confirmed patentency of the esophageal anastomoses. Histopathology showed a circular anastomosis lined with contiguous epithelium. MECA creates a long-term functional and patent anastomosis in pigs. This concept may facilitate minimally-invasive esophageal atresia repair by obviating a technically challenging and time-consuming hand-sewn anastomosis.

Sections du résumé

BACKGROUND/PURPOSE OBJECTIVE
Previous studies have shown that a patent, watertight esophageal anastomosis can be accomplished safely using specially-shaped magnets in piglets. However, it is unclear whether such a magnetic esophageal compression anastomosis (MECA) remains patent in the long-term. The purpose of this study was to evaluate the long-term outcome of MECA in an experimental pig model over an observation period of 2 months.
METHODS METHODS
Ten piglets underwent creation of an MECA with custom-made 8 mm magnets and a U-shaped esophageal bypass loop to allow peroral nutrition at eight weeks of life. Two weeks later, the bypass loop was closed surgically, requiring the pigs to swallow via the newly created magnetic compression anastomosis. The pigs were fed soft chow for 2 months. They were monitored for weight gain and signs of dysphagia. At the endpoint of two months, esophagoscopy and contrast esophagography was performed. After removal of the esophagus, the tissues were macroscopiocally and histologically assessed.
RESULTS RESULTS
Six piglets survived until the endpoint. In two pigs, closure of the bypass loop failed, these demonstrated mean weight gain of 792 gs/day [95% Confidence interval 575 to 1009 gs/day]. Weight gain in four pigs that exclusively fed via the magnetic anastomosis averaged 577 gs/day [95% confidence interval 434 to 719 gs/day (p = 0.18)]. There were no signs of dysphagia. All magnets passed with the stool within 16 days. After 2 months, a well-formed magnetic compression anastomosis was visible and easily negotiated with a 6.5 mm endoscope. Esophogram and macroscopic findings confirmed patentency of the esophageal anastomoses. Histopathology showed a circular anastomosis lined with contiguous epithelium.
CONCLUSION CONCLUSIONS
MECA creates a long-term functional and patent anastomosis in pigs. This concept may facilitate minimally-invasive esophageal atresia repair by obviating a technically challenging and time-consuming hand-sewn anastomosis.

Identifiants

pubmed: 34656308
pii: S0022-3468(21)00646-1
doi: 10.1016/j.jpedsurg.2021.09.032
pii:
doi:

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

34-40

Informations de copyright

Copyright © 2021 Elsevier Inc. All rights reserved.

Auteurs

Alexander Sterlin (A)

Department of Pediatric Surgery, Johannes Gutenberg University Mainz, Mainz, Germany.

Lauren Evans (L)

Department of Pediatric Surgery, University of California at San Francisco, San Francisco, California, United States.

Sara Mahler (S)

Department of Pediatric Surgery, Johannes Gutenberg University Mainz, Mainz, Germany.

Andreas Lindner (A)

Department of Pediatric Surgery, Johannes Gutenberg University Mainz, Mainz, Germany.

Jana Dickmann (J)

Department of Veterinary Medicine, Johannes Gutenberg University Mainz, Mainz, Germany.

Axel Heimann (A)

Department of Veterinary Medicine, Johannes Gutenberg University Mainz, Mainz, Germany.

Mohammad Sahlabadi (M)

Department of Pediatric Surgery, University of California at San Francisco, San Francisco, California, United States.

Vamsi Aribindi (V)

Department of Pediatric Surgery, University of California at San Francisco, San Francisco, California, United States.

Michael R Harrison (MR)

Department of Pediatric Surgery, University of California at San Francisco, San Francisco, California, United States; Magnamosis, Inc., San Francisco, California, United States.

Oliver J Muensterer (OJ)

Department of Pediatric Surgery, Johannes Gutenberg University Mainz, Mainz, Germany; Department of Pediatric Surgery, Dr. von Hauner Children's Hospital, Ludwig-Maximilians-University of Munich, Lindwurmstrasse 4, Munich 80337, Germany. Electronic address: oliver.muensterer@med.uni-muenchen.de.

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Classifications MeSH