Binding of de novo synthesized radiolabeled juvenile hormone (JH III) by JH receptors from the Cuban subterranean termite Prorhinotermes simplex and the German cockroach Blattella germanica.
Cockroach
Hormone receptor
Juvenile hormone
Ligand binding
Methoprene-tolerant
Termite
Journal
Insect biochemistry and molecular biology
ISSN: 1879-0240
Titre abrégé: Insect Biochem Mol Biol
Pays: England
ID NLM: 9207282
Informations de publication
Date de publication:
12 2021
12 2021
Historique:
received:
01
10
2021
revised:
11
10
2021
accepted:
11
10
2021
pubmed:
18
10
2021
medline:
16
12
2021
entrez:
17
10
2021
Statut:
ppublish
Résumé
Juvenile hormone (JH) controls insect reproduction and development through an intracellular receptor complex comprising two bHLH-PAS proteins, the JH-binding Methoprene-tolerant (Met) and its partner Taiman (Tai). Many hemimetabolous insects including cockroaches strictly depend on JH for stimulation of vitellogenesis. In termites, the eusocial hemimetabolans, JH also regulates the development of caste polyphenism. Studies addressing the agonist ligand binding to recombinant JH receptors currently include three species belonging to two holometabolous insect orders, but none that would represent any of the hemimetabolous orders. Here, we examined JH receptors in two representatives of Blattodea, the cockroach Blattella germanica and the termite Prorhinotermes simplex. To test the JH-binding capacity of Met proteins from these species, we performed chemical synthesis and tritium labeling of the natural blattodean JH homolog, JH III. Our improved protocol increased the yield and specific activity of [10-
Identifiants
pubmed: 34656795
pii: S0965-1748(21)00154-5
doi: 10.1016/j.ibmb.2021.103671
pii:
doi:
Substances chimiques
Insect Proteins
0
Sesquiterpenes
0
juvenile hormone III
B74U6BJ6J5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
103671Informations de copyright
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