Organoids in image-based phenotypic chemical screens.
Journal
Experimental & molecular medicine
ISSN: 2092-6413
Titre abrégé: Exp Mol Med
Pays: United States
ID NLM: 9607880
Informations de publication
Date de publication:
10 2021
10 2021
Historique:
received:
16
11
2020
accepted:
04
05
2021
revised:
08
04
2021
pubmed:
20
10
2021
medline:
5
4
2022
entrez:
19
10
2021
Statut:
ppublish
Résumé
Image-based phenotypic screening relies on the extraction of multivariate information from cells cultured under a large variety of conditions. Technical advances in high-throughput microscopy enable screening in increasingly complex and biologically relevant model systems. To this end, organoids hold great potential for high-content screening because they recapitulate many aspects of parent tissues and can be derived from patient material. However, screening is substantially more difficult in organoids than in classical cell lines from both technical and analytical standpoints. In this review, we present an overview of studies employing organoids for screening applications. We discuss the promises and challenges of small-molecule treatments in organoids and give practical advice on designing, running, and analyzing high-content organoid-based phenotypic screens.
Identifiants
pubmed: 34663938
doi: 10.1038/s12276-021-00641-8
pii: 10.1038/s12276-021-00641-8
pmc: PMC8569209
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Review
Langues
eng
Sous-ensembles de citation
IM
Pagination
1495-1502Informations de copyright
© 2021. The Author(s).
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