Dysregulation of mitochondrial sirtuin genes is associated with human male infertility.


Journal

Andrologia
ISSN: 1439-0272
Titre abrégé: Andrologia
Pays: Germany
ID NLM: 0423506

Informations de publication

Date de publication:
Feb 2022
Historique:
revised: 22 08 2021
received: 17 06 2021
accepted: 29 09 2021
pubmed: 20 10 2021
medline: 12 1 2022
entrez: 19 10 2021
Statut: ppublish

Résumé

Mitochondrial sirtuins (SIRT3, SIRT4, SIRT5) are post-translational modifiers that regulate energy production, body homeostasis and mitochondrial activities via different substrates in response to environmental stressors. The present study aimed at assessing the expression of SIRT3, SIRT4, and SIRT5 in the semen of infertile men. Expression analysis was performed using q-RT PCR. All mitochondrial sirtuin genes were significantly down-regulated in the semen of infertile men compared to fertile men. Mitochondrial sirtuin genes expression levels were correlated with mitochondrial HSP90 expression. HSP90 expression was positively correlated with SIRT3, SIRT4 and SIRT5 expression in the semen of fertile men, while a negative correlation was observed between HSP90 in the semen of infertile men and mitochondrial sirtuin genes in the semen of fertile men. These data suggest that dysregulation of mitochondrial sirtuin genes causes mitochondrial dysfunction due to stress, which appears to be associated with human male infertility by compromising functional and structural sperm integrity.

Identifiants

pubmed: 34664305
doi: 10.1111/and.14274
doi:

Substances chimiques

Mitochondrial Proteins 0
SIRT3 protein, human EC 3.5.1.-
SIRT4 protein, human EC 3.5.1.-
SIRT5 protein, human EC 3.5.1.-
Sirtuin 3 EC 3.5.1.-
Sirtuins EC 3.5.1.-

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

e14274

Subventions

Organisme : Higher Education Commission of Pakistan
ID : NRPU 3729

Informations de copyright

© 2021 Wiley-VCH GmbH.

Références

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Auteurs

Jaafar Haris Bello (JH)

Department of Biosciences, Functional Genomics and Proteomics Lab, COMSATS University Islamabad, Islamabad, Pakistan.

Muhammad Jadoon Khan (MJ)

Department of Biosciences, Functional Genomics and Proteomics Lab, COMSATS University Islamabad, Islamabad, Pakistan.

Saira Amir (S)

Department of Biosciences, Functional Genomics and Proteomics Lab, COMSATS University Islamabad, Islamabad, Pakistan.

Hoor Gulalai Kakakhel (HG)

Department of Biosciences, Functional Genomics and Proteomics Lab, COMSATS University Islamabad, Islamabad, Pakistan.

Faheem Tahir (F)

Department of Chemical Pathology and Endocrinology, Public Health Laboratories Division, National Institute of Health, Islamabad, Pakistan.

Sikandar Sultan (S)

Department of Chemical Pathology and Endocrinology, Public Health Laboratories Division, National Institute of Health, Islamabad, Pakistan.

Syed Qasim Raza (SQ)

Institute of Biochemistry and Biotechnology, University of Veterinary and Animal Sciences, Lahore, Pakistan.

Charalampos Mamoulakis (C)

Department of Urology, Medical School, University General Hospital of Heraklion, University of Crete, Heraklion, Greece.

Athanasios Zachariou (A)

Department of Urology, Ioannina University School of Medicine, Ioannina, Greece.

Aristidis Tsatsakis (A)

Department of Forensic Sciences and Toxicology, Faculty of Medicine, University of Crete, Heraklion, Greece.

Nikolaos Sofikitis (N)

Department of Urology, Ioannina University School of Medicine, Ioannina, Greece.

Syed Tahir Abbas Shah (STA)

Department of Biosciences, Functional Genomics and Proteomics Lab, COMSATS University Islamabad, Islamabad, Pakistan.

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