Is muscle and protein loss relevant in long-term fasting in healthy men? A prospective trial on physiological adaptations.

Apelin Body composition Energy metabolism Lipids Long-term fasting Muscle function

Journal

Journal of cachexia, sarcopenia and muscle
ISSN: 2190-6009
Titre abrégé: J Cachexia Sarcopenia Muscle
Pays: Germany
ID NLM: 101552883

Informations de publication

Date de publication:
12 2021
Historique:
revised: 23 06 2021
received: 16 11 2020
accepted: 10 07 2021
pubmed: 21 10 2021
medline: 29 1 2022
entrez: 20 10 2021
Statut: ppublish

Résumé

Fasting is attracting an increasing interest as a potential strategy for managing diseases, including metabolic disorders and complementary cancer therapy. Despite concerns of clinicians regarding protein catabolism and muscle loss, evidence-based clinical data in response to long-term fasting in healthy humans are scarce. The objective of this study was to measure clinical constants, metabolic, and muscular response in healthy men during and after a 10 day fast combined with a physical activity programme. Sixteen men (44 ± 14 years; 26.2 ± 0.9 kg/m The 10 day fast decreased BW by 7% (-5.9 ± 0.2 kg, P < 0.001) and BMR by 12% (P < 0.01). Fat mass and lean soft tissues (LST) accounted for about 40% and 60% of weight loss, respectively, -2.3 ± 0.18 kg and -3.53 ± 0.13 kg, P < 0.001. LST loss was explained by the reduction in extracellular water (44%), muscle and liver glycogen and associated water (14%), and metabolic active lean tissue (42%). Plasma 3-methyl-histidine increased until Day 5 of fasting and then decreased, suggesting that protein sparing might follow early proteolysis. Daily steps count increased by 60% (P < 0.001) during the fasting period. Strength was maintained in non-weight-bearing muscles and increased in weight-bearing muscles (+33%, P < 0.001). Glycaemia, insulinemia, blood lipids, and blood pressure dropped during the fast (P < 0.05 for all), while non-esterified fatty acids and urinary beta-hydroxybutyrate increased (P < 0.01 for both). After a transient reduction, inflammatory cytokines returned to baseline at Day 10 of fasting, and LST were still lower than baseline values (-2.3% and -3.2%, respectively; P < 0.05 for both). A 10 day fast appears safe in healthy humans. Protein loss occurs in early fast but decreases as ketogenesis increases. Fasting combined with physical activity does not negatively impact muscle function. Future studies will need to confirm these first findings.

Sections du résumé

BACKGROUND
Fasting is attracting an increasing interest as a potential strategy for managing diseases, including metabolic disorders and complementary cancer therapy. Despite concerns of clinicians regarding protein catabolism and muscle loss, evidence-based clinical data in response to long-term fasting in healthy humans are scarce. The objective of this study was to measure clinical constants, metabolic, and muscular response in healthy men during and after a 10 day fast combined with a physical activity programme.
METHODS
Sixteen men (44 ± 14 years; 26.2 ± 0.9 kg/m
RESULTS
The 10 day fast decreased BW by 7% (-5.9 ± 0.2 kg, P < 0.001) and BMR by 12% (P < 0.01). Fat mass and lean soft tissues (LST) accounted for about 40% and 60% of weight loss, respectively, -2.3 ± 0.18 kg and -3.53 ± 0.13 kg, P < 0.001. LST loss was explained by the reduction in extracellular water (44%), muscle and liver glycogen and associated water (14%), and metabolic active lean tissue (42%). Plasma 3-methyl-histidine increased until Day 5 of fasting and then decreased, suggesting that protein sparing might follow early proteolysis. Daily steps count increased by 60% (P < 0.001) during the fasting period. Strength was maintained in non-weight-bearing muscles and increased in weight-bearing muscles (+33%, P < 0.001). Glycaemia, insulinemia, blood lipids, and blood pressure dropped during the fast (P < 0.05 for all), while non-esterified fatty acids and urinary beta-hydroxybutyrate increased (P < 0.01 for both). After a transient reduction, inflammatory cytokines returned to baseline at Day 10 of fasting, and LST were still lower than baseline values (-2.3% and -3.2%, respectively; P < 0.05 for both).
CONCLUSIONS
A 10 day fast appears safe in healthy humans. Protein loss occurs in early fast but decreases as ketogenesis increases. Fasting combined with physical activity does not negatively impact muscle function. Future studies will need to confirm these first findings.

Identifiants

pubmed: 34668663
doi: 10.1002/jcsm.12766
pmc: PMC8718030
doi:

Banques de données

DRKS
['DRKS00011165']

Types de publication

Clinical Trial Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1690-1703

Subventions

Organisme : NIDDK NIH HHS
ID : P30 DK048520
Pays : United States

Informations de copyright

© 2021 The Authors. Journal of Cachexia, Sarcopenia and Muscle published by John Wiley & Sons Ltd on behalf of Society on Sarcopenia, Cachexia and Wasting Disorders.

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Auteurs

Claire Laurens (C)

Institut Pluridisciplinaire Hubert Curien, Université de Strasbourg, CNRS, Strasbourg, France.
Centre National d'Etudes Spatiales, Paris, France.

Franziska Grundler (F)

Buchinger Wilhelmi Clinic, Überlingen, Germany.
Charité-Universitätsmedizin Berlin, corporate member of Freie Universität Berlin, Humboldt-Universität zu Berlin, and Berlin Institut of Health, Berlin, Germany.

Anthony Damiot (A)

Institut Pluridisciplinaire Hubert Curien, Université de Strasbourg, CNRS, Strasbourg, France.

Isabelle Chery (I)

Institut Pluridisciplinaire Hubert Curien, Université de Strasbourg, CNRS, Strasbourg, France.

Anne-Laure Le Maho (AL)

Clinique Générale d'Annecy, Annecy, France.

Alexandre Zahariev (A)

Institut Pluridisciplinaire Hubert Curien, Université de Strasbourg, CNRS, Strasbourg, France.

Yvon Le Maho (Y)

Institut Pluridisciplinaire Hubert Curien, Université de Strasbourg, CNRS, Strasbourg, France.
Centre Scientifique de Monaco, Monaco.

Audrey Bergouignan (A)

Institut Pluridisciplinaire Hubert Curien, Université de Strasbourg, CNRS, Strasbourg, France.
Division of Endocrinology, Metabolism and Diabetes, Anschutz Health & Wellness Center, University of Colorado, Anschutz Medical Campus, Aurora, CO, USA.

Guillemette Gauquelin-Koch (G)

Centre National d'Etudes Spatiales, Paris, France.

Chantal Simon (C)

CarMen Laboratory, INSERM 1060, INRA 1397, University of Lyon, Oullins, France.
Human Nutrition Research Centre of Rhône-Alpes, Hospices Civils de Lyon, Lyon, France.

Stéphane Blanc (S)

Institut Pluridisciplinaire Hubert Curien, Université de Strasbourg, CNRS, Strasbourg, France.

Françoise Wilhelmi de Toledo (F)

Buchinger Wilhelmi Clinic, Überlingen, Germany.

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